| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2019: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2016: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
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| Outline of Final Research Achievements |
Amyloid fibrils typically propagate their own structures by a template-dependent growth mechanism, in which the termini of the fibrils serve as a propagation template. To elucidate the property of the template structure and the mechanism of its formation, we focused on “prefibrillar intermediates”, i.e., early aggregates that are assumed to be formed before demonstrating the propagation ability. We have identified a characteristic pathway of the formation of amyloid fibrils of human insulin B chain, in which prefibrillar intermediates populated significantly. We have analyzed the structural properties of the prefibrillar intermediates and tracked their formation in a time-dependent manner. We have also found the inhibition of the B-chain amyloid formation by fibrinogen, which interacted with the prefibrillar intermediates.
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