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Controlling Microtubules: from cells to tissues

Research Project

Project/Area Number 16H04787
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionWaseda University

Principal Investigator

SATO MASAMITSU  早稲田大学, 理工学術院, 教授 (50447356)

Research Collaborator TOYA Mika  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2018: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2016: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywords細胞骨格 / 細胞周期 / 細胞分裂 / 微小管 / 細胞・組織 / 遺伝子 / 遺伝学 / 発生・分化 / 細胞生物学 / 分子生物学
Outline of Final Research Achievements

This study aimed to investigate how microtubule is assembled in cells: the analyses include those at molecular level, cell level and tissue level. We used fission yeast and human cultured cells to analyze how microtubules are formed, and we have been establishing systems for animal experiments. As a result, we found that Alp7/TACC accelerate microtubule nucleation in cooperation with the gamma-tubulin complex. In addition, our results indicate that there may be Alp7-independent microtubule nucleation system operating in fission yeast cells.

Academic Significance and Societal Importance of the Research Achievements

微小管は細胞の中で様々な機能を発揮することが知られている。細胞が分裂していない時期には、微小管は細胞質に網目状に存在して物質輸送のレールとして働く。これに対して分裂する際には、微小管は紡錘体をつくり、染色体を2つの細胞に分配する機能を担う。すなわち、正しい時期に紡錘体が正しく形成されないと、染色体の分配に異常が起き、がん化や細胞死の原因となる。そこで本研究では微小管が時期によってかたちを変化させる仕組みを調べた結果、Alp7/TACCタンパク質が時期により制御を受けることで、微小管が適切な時期に適切な場所で形成されることを解明した。現在は酵母に加えてヒトなどの高等生物でも研究を続けている。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (32 results)

All 2018 2017 2016 Other

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (27 results) (of which Int'l Joint Research: 14 results,  Invited: 1 results) Remarks (3 results)

  • [Journal Article] Module-based systematic construction of plasmids for episomal gene expression in fission yeast2017

    • Author(s)
      Kiriya Keita、Tsuyuzaki Hayato、Sato Masamitsu
    • Journal Title

      Gene

      Volume: 637 Pages: 14-24

    • DOI

      10.1016/j.gene.2017.09.030

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reconstructing regulatory pathways by systematically mapping protein localization interdependency networks2017

    • Author(s)
      Dodgson James、Chessel Anatole、Vaggi Federico、Giordan Marco、Yamamoto Miki、Arai Kunio、Madrid Marisa、Geymonat Marco、Abenza Juan Francisco、Cansado Jose、Sato Masamitsu、Csikasz-Nagy Attila、Carazo-Salas Rafael E
    • Journal Title

      bioRxiv

      Volume: - Pages: 116749-116749

    • DOI

      10.1101/116749

    • Related Report
      2016 Annual Research Report
    • Open Access / Int'l Joint Research
  • [Presentation] CDK-dependent nuclear accumulation of Alp7/TACC promotes the assembly of the radial array of microtubules in meiosis I.2018

    • Author(s)
      Yutaka Shirasugi, Masamitsu Sato
    • Organizer
      第70回細胞生物学会第51回日本発生生物学会合同大会
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional analysis of fission yeast CLASP in assembling pre-anaphase spindle2018

    • Author(s)
      Hirohisa Ebina, Liang Ji, Masamitsu Sato
    • Organizer
      第70回細胞生物学会第51回日本発生生物学会合同大会
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional linkage between the γ-tubulin ring complex and Alp7/TACC in microtubule nucleation2018

    • Author(s)
      Mana Katsuyama, Tomonari Sunaga, Masamitsu Sato
    • Organizer
      第70回細胞生物学会第51回日本発生生物学会合同大会
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Organization of microtubules in small intestinal crypt cells2018

    • Author(s)
      Yuto Mitsuhata, Mika Toya, Masatoshi Takeichi, Masamitsu Sato
    • Organizer
      第70回細胞生物学会第51回日本発生生物学会合同大会
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Mis6/CENP-IはセントロメアからノンコーディングRNAが転写される際にCENP-Aを維持する2018

    • Author(s)
      平井隼人、佐藤政充
    • Organizer
      第41回日本分子生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 機能性non-coding RNAの二次構造に基づく探索2018

    • Author(s)
      片山研太, 浜田道昭, 佐藤政充
    • Organizer
      第41回日本分子生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] An Unconventional Function of Dis1 as a Microtubule Depolymerizer2018

    • Author(s)
      Yuichi Murase, Takahiro Hamada, Masamitsu Sato
    • Organizer
      5th Core-to-Core International Symposium "3D Lab-Exchange Program"
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Functional analysis for the microtubule minus-end binding protein CAMSAP2 in spindle assembly2018

    • Author(s)
      Naoko Nishizawa, Mika Toya, Masatoshi Takeichi, Masamitsu Sato
    • Organizer
      5th Core-to-Core International Symposium "3D Lab-Exchange Program"
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] How Microtubule is Organized during the Cell Cycle in Fission Yeast2017

    • Author(s)
      佐藤政充,新井邦生,大石愛佳,須永智成
    • Organizer
      第69回日本細胞生物学会大会
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 細胞周期の時期に応じて微小管の形成部位が切り替わるメカニズムの解明2017

    • Author(s)
      大石愛佳、新井邦生、須永智成、佐藤政充
    • Organizer
      第69回日本細胞生物学会大会
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 分裂酵母CENP-Aがセントロメアに呼び込まれる機構の解明2017

    • Author(s)
      分裂酵母CENP-Aがセントロメアに呼び込まれる機構の解明
    • Organizer
      第2回クロマチン動構造ワークショップ
    • Related Report
      2017 Annual Research Report
  • [Presentation] 分裂酵母β-tubulin変異体nda3-TB101における微小管安定化メカニズムの解析2017

    • Author(s)
      白戸みな萌、桐谷啓太、佐藤政充
    • Organizer
      生命科学系学会合同年次大会 第40回日本分子生物学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] non-coding RNAの二次構造による網羅的分類2017

    • Author(s)
      片山研太、田頭将喜、浜田道昭、佐藤政充
    • Organizer
      生命科学系学会合同年次大会 第40回日本分子生物学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 減数第一分裂の開始時に 放射状の微小管が形成する分子機構の解明2017

    • Author(s)
      白杉豊、佐藤政充
    • Organizer
      第35回染色体ワークショップ
    • Related Report
      2017 Annual Research Report
  • [Presentation] 分裂酵母Mis6/CENP-Iはセントロメアにおける CENP-Aの維持に寄与する2017

    • Author(s)
      平井隼人、佐藤政充
    • Organizer
      第35回染色体ワークショップ
    • Related Report
      2017 Annual Research Report
  • [Presentation] Transcription Profiling from a Single pombe Cell2017

    • Author(s)
      佐藤政充
    • Organizer
      10th HiHA international Workshop Cell Cycle Control and beyond
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 分裂酵母CENP-Aの呼び込みは ヒストンH3のアセチル化修飾により阻害される2017

    • Author(s)
      平井隼人、佐藤政充
    • Organizer
      第34回染色体ワークショップ
    • Related Report
      2016 Annual Research Report
  • [Presentation] 分裂酵母CENP-Aがセントロメアに呼び込まれるメカニズムの解明2016

    • Author(s)
      平井隼人,佐藤政充
    • Organizer
      第1回クロマチン動構造ワークショップ
    • Related Report
      2016 Annual Research Report
  • [Presentation] 減数第一分裂において微小管を脱重合する機構の解明2016

    • Author(s)
      齋藤俊一、角井康貢、佐藤政充
    • Organizer
      第49回酵母遺伝学フォーラム研究報告会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 減数第一・第二分裂間のSPB複製機構の解明2016

    • Author(s)
      石井 陽菜、佐藤 政充
    • Organizer
      第49回酵母遺伝学フォーラム研究報告会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 減数分裂が温度感受性を示す原因の特定2016

    • Author(s)
      齋藤 航、佐藤政充
    • Organizer
      第49回酵母遺伝学フォーラム研究報告会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Interaction between Alp7/TACC and g-TuC regulators, Mto1 and Pcp1, is required for microtubule organization in fission yeast.2016

    • Author(s)
      Kunio Arai, Tomonari Sunaga, Naoyuki Okada, Masamitsu Sato
    • Organizer
      EMBO Microtubule Conference
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Dis1 and Dam1 depolymerize Microtubules during meiotic prophase in fission yeast.2016

    • Author(s)
      Shunichi Saito, Yasutaka Kakui and Masamitsu Sato
    • Organizer
      EMBO Microtubule Conference
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Microtubule nucleation is stimulated by Alp7/TACC in fission yeast2016

    • Author(s)
      Tomonari Sunaga, Kunio Arai, Naoyuki Okada and Masamitsu Sato
    • Organizer
      EMBO Microtubule Conference
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Two TOG proteins differently drive microtubule dynamics in fission yeast2016

    • Author(s)
      Shunichi Saito, Ryosuke Dairoku, Yasutaka Kakui and Masamitsu Sato
    • Organizer
      EMBO Microtubule Conference
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] How microtubule cytoskeleton is reorganized during the cell cycle2016

    • Author(s)
      Masamistu Sato
    • Organizer
      3D Lab Exchange Symposium
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 多剤耐性(MDR)抑圧株酵母を用いた減数第一分裂時の動原体の精製2016

    • Author(s)
      白杉豊、佐藤政充
    • Organizer
      第39回日本分子生物学会年会
    • Related Report
      2016 Annual Research Report
  • [Remarks] 佐藤研究室website

    • Related Report
      2018 Annual Research Report
  • [Remarks] 佐藤研究室 研究内容

    • URL

      http://www.sato.biomed.sci.waseda.ac.jp/intro.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 佐藤研究室 研究内容

    • URL

      http://www.sato.biomed.sci.waseda.ac.jp/intro.html

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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