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Elucidation of the novel adaptation mechanism for winter environment via vascular bundle in perennial plants

Research Project

Project/Area Number 16H04801
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionUniversity of Tsukuba

Principal Investigator

SATOH Shinobu  筑波大学, 生命環境系(副学長), 副学長 (70196236)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Keywordsポプラ / 導管液 / 冬季環境 / 耐性 / 根 / 落葉樹 / 短日 / microRNA / 篩管 / 葉 / 休眠 / 低温馴化 / 糖 / ショ糖 / ショ糖輸送体 / 低温
Outline of Final Research Achievements

A xylem sap protein (XSP24), which is abundant in the xylem sap of poplar in winter, belongs to the Cupin family, and its expression is induced by short-day (SD) and ABA application to root, and is considered to contribute to the removal of ROS at low temperatures in winter. Also, XSP25 belongs to the Basic Secretory Protein family, expression of which is induced in the central cylinder of the root in SD (not induced by ABA), and XSP25 protein accumulation is observed in buds, and may protect the cells from freeze & dry. In addition, several microRNAs expressed in SD leaves and contained in the known phloem sap microRNA database such as squash were identified as candidates for signaling factors to roots.

Academic Significance and Societal Importance of the Research Achievements

落葉性木本植物において秋の短日は、休眠芽形成と冬季環境への適応を誘導する。冬期導管液中のタンパク質(XSP25、XSP24)は根から地上部に送られて冬期の低温や乾燥に対する耐性をもたらすと考えられた。また無菌ポット栽培では、短日条件下で根の成長が低下し、根でXSP25、XSP24、ショ糖排出輸送体(SWEETs)の発現が誘導された。しかし短日において休眠芽形成を誘導するABAはXSPsとSWEETsの一部しか誘導しなかった。一方、短日の葉で発現し、篩管液に含まれる可能性のあるmicroRNA が複数同定された。この結果は、短日情報を葉から根へ伝達する新たな因子の発見につながることが期待される。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (15 results)

All 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (4 results) Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (5 results) Remarks (3 results)

  • [Int'l Joint Research] University of Ottawa(カナダ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Southern Cross University(オーストラリア)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] オタワ大学(カナダ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] タスマニア大学(オーストラリア)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Presence of a basic secretory protein in xylem sap and shoots of poplar in winter and its physicochemical activities against winter environmental conditions2019

    • Author(s)
      T. Aohara, J. Furukawa, K. Miura, S. Tsuda, J. S. Poisson, R. N. Ben, P. W. Wilson, S. Satoh
    • Journal Title

      J. Plant Research

      Volume: 132 Issue: 5 Pages: 655-665

    • DOI

      10.1007/s10265-019-01123-9

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] RAP2.6L and jasmonic acid–responsive genes are expressed upon Arabidopsis hypocotyl grafting but are not needed for cell proliferation related to healing2018

    • Author(s)
      Matsuoka K, Yanagi R, Yumoto E, Yokota T, Yamane H, Satoh S, Asahina M
    • Journal Title

      Plant Molecular Biology

      Volume: 96 Issue: 6 Pages: 531

    • DOI

      10.1007/s11103-018-0702-4

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of a xylem sap germin-like protein and its expression under short-day and non-freezing low-temperature conditions in poplar root2016

    • Author(s)
      Aohara T., Mizuno H., Kiyomichi D., Abe Y., Matsuki K., Sagawa K., Mori H., Iwai H., Furukawa J., Satoh S.
    • Journal Title

      Plant Biotechnology

      Volume: 33 Issue: 2 Pages: 123-127

    • DOI

      10.5511/plantbiotechnology.16.0411a

    • NAID

      130005164807

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] ポプラの短日による休眠移行におけるmicroRNAの根への長距離輸送と機能2020

    • Author(s)
      廣岡慎也、小野公代、古川純、小野道之、佐藤忍
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] シロイヌナズナ切断花茎におけるエクスパンシンの発現と機能の解析2018

    • Author(s)
      横川裕理、ハニエビダディ、小野公代、松岡啓太、朝比奈雅志、岩井宏暁、佐藤忍
    • Organizer
      日本植物生理学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ポプラの季節性成長における糖含量の変化とショ糖排出トランスポーターの組織化学的発現解析2017

    • Author(s)
      小野公代、鈴木陽佳,青原勉,小野道之、岩井宏暁,古川純、佐藤忍
    • Organizer
      日本植物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ポプラの根で生産された細胞外導管液タンパク質の耐凍性への関与2017

    • Author(s)
      青原勉、古川純、三浦謙治、佐藤忍
    • Organizer
      日本植物生理学会
    • Place of Presentation
      鹿児島大学(鹿児島県・鹿児島市)
    • Related Report
      2016 Annual Research Report
  • [Presentation] ポプラ根の成長と導管液中の糖に関連する遺伝子の発現に対する日長及び温度の効果2016

    • Author(s)
      鈴木陽佳、青原勉、古川純、佐藤忍
    • Organizer
      日本植物学会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県・宜野湾市)
    • Related Report
      2016 Annual Research Report
  • [Remarks] ここは佐藤忍研究室です

    • URL

      http://www.biol.tsukuba.ac.jp/~plphys/shinobuhomepage/SSindex.html

    • Related Report
      2019 Annual Research Report 2016 Annual Research Report
  • [Remarks]

    • URL

      http://www.biol.tsukuba.ac.jp/~plphys/shinobuhomepage/SSindex.html

    • Related Report
      2018 Annual Research Report
  • [Remarks] ここは 佐藤 忍 研究室です

    • URL

      http://www.biol.tsukuba.ac.jp/~plphys/shinobuhomepage/SSindex.html

    • Related Report
      2017 Annual Research Report

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Published: 2016-04-21   Modified: 2021-02-19  

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