Comparative analysis of cytoskeletal function in plant cell division and morphogenesis using new standard cell lines and image processing
| Project/Area Number |
16H04802
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
HASEBE Mitsuyasu 基礎生物学研究所, 発生遺伝学研究部門, 教授 (40237996)
KOUCHI Takayuki 京都大学, 生命科学研究科, 教授 (40202056)
KUTSUNA Natsumaro 東京大学, 大学院新領域創成科学研究科, 特任准教授 (70578559)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Keywords | 植物細胞分裂・形態形成 / 細胞骨格 / 画像定量解析 / standard cell line |
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| Outline of Final Research Achievements |
We have tried to newly establish standard cell lines (SCLs) suitable for various analysis needs of cell research in fern and moss plants. Further, we have also tried to develop new unique system for single-cell comparative quantitative analyses. The results are as follows. We established a fern SCL candidate and commissioned it to RIKEN BRC. Using this cell line, the practical protoplast preparation method was established, and the short report was published. The observations of the cell division apparatus by fluorescent antibody staining or GFP were performed. Also, we developed a new image analysis system and published the paper.
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| Academic Significance and Societal Importance of the Research Achievements |
新規開発のSCLと画像解析ツールのいずれについても波及効果は著しいと考えられる。今後、これらによる被子植物、シダ植物、コケ植物の細胞現象の比較解析により得られる成果が期待される一方で、本研究で開発されたシングルセル画像解析ツールは幅の広い目的に使用できるものが完成しており、他の研究分野への応用も大いに期待される。
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Differential Effects of Phosphatidylinositol 4-Kinase (PI4K) and 3-Kinase (PI3K) Inhibitors on Stomatal Responses to Environmental Signals.2017
Author(s)
Takahashi, S., Monda, K., Higaki, T., Negi, J., Hashimoto-Sugimoto, M., Hasezawa, S. and Iba, K.
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Journal Title
Front. Plant Sci.
Volume: 8
Pages: 677-677
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Accumulation of fluorescent proteins derived from a trans-Golgi cisternal membrane marker and paramural bodies in interdigitated apoplastic space in Arabidopsis leaf epidermis2016
Author(s)
Akita, K., Kobayashi, M., Sato, M., Kutsuna, N., Ueda, T., Toyooka, K., Nagata, N., Hasezawa, S., and Higaki, T.
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Journal Title
Protoplasma
Volume: in press
Issue: 1
Pages: 367-377
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Cytoskeleton dynamics control the first asymmetric cell division in Arabidopsis zygote.2016
Author(s)
Kimata, Y., Higaki, T., Kawashima, T., Kurihara, D., Sato, Y., Yamada, T., Hasezawa, S., Berger, F., Higashiyama, T., and Ueda, M.
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Journal Title
Proceedings of the National Academy of Sciences
Volume: 113
Issue: 49
Pages: 14157-14162
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Dominant and recessive mutations in the Raf-like kinase HT1 gene completely disrupt stomatal responses to CO2.2016
Author(s)
Hashimoto-Sugimoto, M., Negi, J., Monda, K., Higaki, T., Isogai, Y., Nakano, T., Hasezawa, S. and Iba, K.
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Journal Title
J.Exp. Bot.
Volume: 未定
Issue: 11
Pages: 3251-3261
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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