Molecular mechanism of photosynthetic acclimation by sensing environmental carbon and nitrogen
| Project/Area Number |
16H04805
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Keywords | 二酸化炭素 / 緑藻クラミドモナス / 光合成 / CO2濃縮機構 / タンパク質リン酸化酵素 / リン酸化プロテオーム解析 / 栄養環境 / ピレノイド / 無機炭素濃縮 / クロロフィル / カルシウム / 窒素シグナル / タンパク質リン酸化 / 無機炭素濃縮機構 / 配偶子誘導 / チラコイド / 葉緑体 / シグナル / 無機炭素輸送体 / 緑藻 / カルシウムシグナル / 環境順化 / シグナル伝達 / 窒素飢餓 / レトログレードシグナル |
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| Outline of Final Research Achievements |
Photosynthetic organisms survive by controlling the photosynthesis and reproduction by detecting deficiency of inorganic nutrients. We found factors involved in this environmental acclimation from the green alga Chlamydomonas. 1) Induction of the CO2-concentrating mechanism (CCM) essential for the maintenance of photosynthesis requires translocation of the post-transcriptional calcium sensor protein CAS into the pyrenoid. 2) A starch sheath around the "pyrenoid" of CO2-fixing enzymes is required for CCM, separating the compartments for CO2-fixing and O2-oxygen evolution. 3) In nitrogen starvation, DYRK-type protein phosphatase TAR1 promotes ROS generation, induces degradation of photosynthesis-related proteins and gametogenesis, and leads to lipid accumulation. 4) Autophagy is required for starch accumulation and maintenance by photosynthesis.
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| Academic Significance and Societal Importance of the Research Achievements |
光合成は、全ての生物の生存を支える基本反応であるが、光合成生物は環境の変化に応じて自ら光合成反応を制御することで生存を図っている。特に光合成基質の二酸化炭素は水中では枯渇することになるので、光合成維持には無機炭素濃縮機構が光強度に応じて誘導される。また、無機栄養源の枯渇によっても光合成は制御を受ける。今回、新しい光合成の制御因子（CAS, TAR1, デンプン鞘、オートファジー）を明らかにしたことで、生命維持機構の理解に貢献し、食糧生産に資する植物分子育種に新しい技術の可能性を示した。
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Report
(4 results)
Research Products
(57 results)
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[Journal Article] The bZIP transcription factor regulates lipid remodeling and contributes to ER stress management in Chlamydomonas reinhardtii.2019
Author(s)
Yamaoka Y, Shin S, Choi BY, Kim H, Jang S, Kajikawa M, Yamano T, Kong F, Legeret B, Fukuzawa H, Li-Beisson Y, Lee Y.
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Journal Title
Plant Cell
Volume: 31
Issue: 5
Pages: 1127-1140
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Algal Protein Kinase, Triacylglycerol Accumulation Regulator 1, Modulates Cell Viability and Gametogenesis in Carbon/Nitrogen-Imbalanced Conditions2019
Author(s)
Shinkawa H, Kajikawa M, Nomura Y, Ogura M, Sawaragi Y, Yamano T, Nakagami H, Sugiyama N, Ishihama Y, Kanesaki Y, Yoshikawa H, Fukuzawa H
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Journal Title
Plant and Cell Physiology
Volume: 60
Issue: 4
Pages: 916-930
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Isolation and characterization of Chlamydomonas autophagy-related mutants in nutrient-deficient conditions.2018
Author(s)
Kajikawa, M., Yamauchi, M., Shinkawa, H., Tanaka, M., Hatano, K., Nishimura, Y., Kato, M., Fukuzawa, H.
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Journal Title
Plant Cell Physiology
Volume: 60
Issue: 1
Pages: 126-138
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Chloroplast-mediated regulation of CO2-concentrating mechanism by Ca2+-binding protein CAS in the green alga Chlamydomonas reinhardtii2016
Author(s)
L. Wang, T. Yamano, S. Takane, Y. Niikawa, C. Toyokawa, S. Ozawa, R. Tokutsu, Y. Takahashi, J. Minagawa, Y. Kanesaki, H. Yoshikawa, and H. Fukuzawa
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 113
Issue: 44
Pages: 12586-12591
DOI
NAID
Related Report
Peer Reviewed / Open Access
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