Elucidation of the molecular mechanisms underlying formation of the plant cuticle
| Project/Area Number |
16H04806
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Meiji University (2018-2020)
Osaka University (2016-2017) |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2020: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
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| Keywords | クチクラ / ABCトランスポーター / 細胞膜 / 輸送体 / 膜交通 / 小胞輸送 / シロイヌナズナ / 表皮分化 / ABC輸送体 / 植物分子 / オルガネラ・細胞壁 / 植物 / 細胞・組織 / トランスゴルジ網 / 細胞、組織 / 発生・分化 |
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| Outline of Final Research Achievements |
The plant cuticle covers aerial organs such as leaves, stem and floral organs, and plays essential roles in water retention, defense against pathogen, and prevention of organ fusion during their development. In the present research, we have utilized an Arabidopsis ABC transporter ABCG11, which is mainly expressed in epidermis and promote cuticle formation. According to the previous studies, ABCG11 localizes at the plasma membrane of the epidermal cells and export cuticle precursor. Therefore, it is plausible to speculate that localization of ABCG11 at the plasma membrane is a prerequisite for its functionality. In order to understand the mechanisms involved in intracellular trafficking of ABCG11, we have carried out screening for the sequence-based determinant for ABCG11 localization, and genetic screen for regulatory components involved in ABCG11 localization.
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| Academic Significance and Societal Importance of the Research Achievements |
シロイヌナズナのABCG11は、地上部の器官のクチクラ形成において、クチクラの主な構成要素であるクチンとワックスの両方の輸送に関わると考えられている極めて重要な輸送体である。ABCG11は細胞膜に局在することが適切に機能するために必要であると考えられるが、細胞膜にABCG11を分布されるためのしくみは十分に理解されていなかった。本研究ではABCG11の細胞膜局在に関与しうる制御因子の候補や、ABCG11の細胞膜局在に必要なアミノ酸配列を同定した。本研究で得られた変異型ABCG11や、制御因子などの研究材料を活用した研究を継続することで、ABCG11の局在制御機構の理解が深まると期待される。
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Report
(5 results)
Research Products
(11 results)
