Studies on the differentiation of various cells, which are working in specialized metabolism of the plant.
| Project/Area Number |
16H04807
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Plant molecular biology/Plant physiology
|
|---|
| Research Institution | Kobe University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2016-04-01 – 2019-03-31
|
| Project Status |
Completed (Fiscal Year 2019)
|
| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
|
|---|
| Keywords | 二次代謝 / ニチニチソウ / 異形細胞 / モノテルペノイドインドールアルカロイド / メタボローム / テルペノイドインドールアルカロイド / 乳管細胞 |
|---|
| Outline of Final Research Achievements |
The secondary metabolism of plants is known to be a trans-cellular type in which a series of chemical reactions are not completed within a single cell, but proceed by the complex movement of intermediate metabolites between multiple cells. In this study, we aimed to elucidate the whole picture of metabolic differentiation of various cells in this metabolism: 1) we elucidated the details of the intercellular distribution of metabolites in stems and leaves, 2) gene expression was analyzed to clarify the metabolic differentiation of various cells, and 3) we found that cultured cells do not accumulate secondary metabolites, but express all related enzymatic genes.
|
| Academic Significance and Societal Importance of the Research Achievements |
固着生活を送る植物は、その場の環境に適応するために様々な生理機能を発達させており、その一つが二次代謝である。二次代謝産物は、受粉の媒介に必要な昆虫の誘引、植食動物の忌避など重要な役割を持つ。その生理活性は、人間にも効果を示すものがあり、本研究で扱うニチニチソウ(Catharanthus roseus)は、抗がん剤として利用される化合物を多数合成する。ニチニチソウ代謝産物の生合成経路は詳細に研究されているが、その代謝が植物体内でどのように制御されているかは、未だほとんど知られていない。それらを明らかにすることは、植物の代謝過程の解明としてばかりでなく、人間に有用な物質の産生にも寄与できる。
|
Report
(4 results)
Research Products
(37 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] The RopGEF KARAPPO Is Essential for the Initiation of Vegetative Reproduction in Marchantia polymorpha2019
Author(s)
Hiwatashi T., Goh H., Yasui Y., Koh L.Q., Takami H., Kajikawa M., Kirita H., Kanazawa T., Minamino N., Togawa T., Sato M., Wakazaki M., Yamaguchi K., Shigenobu S., Fukaki H., Mimura T., Toyooka K., Sawa S., Yamato K.T.,Ueda T., Urano D., Kohchi T., Ishizaki K.
-
Journal Title
Current Biology
Volume: 29
Issue: 20
Pages: 3525-3531
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
[Journal Article] GEMMA CUP-ASSOCIATED MYB1, an ortholog of axillary meristem regulators, is essential in vegetative reproduction in Marchantia polymorpha.2019
Author(s)
Yasui Y, Tsukamoto S, Sugaya T, Nishihama R, Wang Q, Kato H, Yamato KT, Fukaki H, Mimura T, Kubo H, Theres K, Kohchi T, Ishizaki K.
-
Journal Title
Curr Biol.
Volume: 29
Issue: 23
Pages: 3987-3995
DOI
NAID
Related Report
Peer Reviewed / Int'l Joint Research
-
[Journal Article] Molecular Components of Arabidopsis Intact Vacuoles Clarified with Metabolomic and Proteomic Analyses2018
Author(s)
Miwa Ohnishi, Aya Anegawa, Yuko Sugiyama, Kazuo Harada, Akira Oikawa, Yasumune Nakayama, Fumio Matsuda, Yukiko Nakamura, Ryosuke Sasaki, Chizuko Shichijo Patrick G. Hatcher, Hidehiro Fukaki, Shigehiko Kanaya, Koh Aoki, Mami Yamazaki, Eiichiro Fukusaki, Kazuki Saito, Tetsuro Mimura
-
Journal Title
Plant and Cell Physiology
Volume: 69
Pages: 1353-1362
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
[Journal Article] Cadmium-induced changes in vacuolar aspects of Arabidopsis thaliana.2017
Author(s)
Shanti S. Sharma, Kotaro Yamamoto, Kohei Hamaji, Miwa Ohnishi, Aya Anegawa, Shashi Sharma, Sveta Thakur, Vijay Kumar, Tomohiro Uemura, Akihiko Nakano, Tetsuro Mimura
-
Journal Title
Plant Physiol Biochem.
Volume: 114
Pages: 29-37
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
[Journal Article] Inositol hexakis phosphate is the seasonal phosphorus reservoir in the deciduous woody plant Populus alba L.2017
Author(s)
Kurita, Y., Baba, K., Ohnishi, M., Matsubara, R., Kosuge, K., Anegawa, A., Shichijo, C., Ishizaki, K., Kaneko, Y., Hayashi, M., Suzaki, T., Fukaki, H., Mimura, T.
-
Journal Title
Plant Cell Physiol.
Volume: 58
Issue: 9
Pages: 1477-1485
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
[Presentation] Changes in secondary metabolites related to plant development in Catharanthus roseus.2019
Author(s)
UZAKI Mai, K. YAMAMOTO Kotaro, TAKAHASHI Katsutoshi, OHNISHI Miwa, KURITA Yuko, SHICHIJO Chizuko, NAGANO Atsushi, ISHIZAKI Kimitsune, FUKAKI Hidehiro, MIMURA Tetsuro
Organizer
4th International Meeting on the Biosynthesis, Function and Synthetic Biology of Isoprenoids
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
