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Studies on the differentiation of various cells, which are working in specialized metabolism of the plant.

Research Project

Project/Area Number 16H04807
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant molecular biology/Plant physiology
Research InstitutionKobe University

Principal Investigator

Mimura Tetsuro  神戸大学, 理学研究科, 教授 (20174120)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywords二次代謝 / ニチニチソウ / 異形細胞 / モノテルペノイドインドールアルカロイド / メタボローム / テルペノイドインドールアルカロイド / 乳管細胞
Outline of Final Research Achievements

The secondary metabolism of plants is known to be a trans-cellular type in which a series of chemical reactions are not completed within a single cell, but proceed by the complex movement of intermediate metabolites between multiple cells. In this study, we aimed to elucidate the whole picture of metabolic differentiation of various cells in this metabolism: 1) we elucidated the details of the intercellular distribution of metabolites in stems and leaves, 2) gene expression was analyzed to clarify the metabolic differentiation of various cells, and 3) we found that cultured cells do not accumulate secondary metabolites, but express all related enzymatic genes.

Academic Significance and Societal Importance of the Research Achievements

固着生活を送る植物は、その場の環境に適応するために様々な生理機能を発達させており、その一つが二次代謝である。二次代謝産物は、受粉の媒介に必要な昆虫の誘引、植食動物の忌避など重要な役割を持つ。その生理活性は、人間にも効果を示すものがあり、本研究で扱うニチニチソウ(Catharanthus roseus)は、抗がん剤として利用される化合物を多数合成する。ニチニチソウ代謝産物の生合成経路は詳細に研究されているが、その代謝が植物体内でどのように制御されているかは、未だほとんど知られていない。それらを明らかにすることは、植物の代謝過程の解明としてばかりでなく、人間に有用な物質の産生にも寄与できる。

Report

(4 results)
  • 2019 Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (37 results)

All 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (12 results) Journal Article (12 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 12 results,  Open Access: 7 results,  Acknowledgement Compliant: 1 results) Presentation (13 results) (of which Int'l Joint Research: 5 results,  Invited: 3 results)

  • [Int'l Joint Research] Max Planck Institute(ドイツ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Sikkim University(インド)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] John Innes Institute(英国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] John Innes Centre(英国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] HImachal Pradesh University(インド)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Bielefeld University(ドイツ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Zurich University(スイス)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] 中興大学(台湾)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] John Innes Centre(英国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] HImachal Pradesh University(インド)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Bielefeld University(ドイツ)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] 中興大学(台湾)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] The complexity of intercellular localisation of alkaloids revealed by single‐cell metabolomics2019

    • Author(s)
      Yamamoto Kotaro、Takahashi Katsutoshi、Caputi Lorenzo、Mizuno Hajime、Rodriguez‐Lopez Carlos E.、Iwasaki Tetsushi、Ishizaki Kimitsune、Fukaki Hidehiro、Ohnishi Miwa、Yamazaki Mami、Masujima Tsutomu、O'Connor Sarah E.、Mimura Tetsuro
    • Journal Title

      New Phytologist

      Volume: 224 Issue: 2 Pages: 848-859

    • DOI

      10.1111/nph.16138

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Lateral root initiation requires the sequential induction of transcription factors LBD16 and PUCHI in Arabidopsis thaliana2019

    • Author(s)
      Goh Tatsuaki、Toyokura Koichi、Yamaguchi Nobutoshi、Okamoto Yoshie、Uehara Takeo、Kaneko Shutaro、Takebayashi Yumiko、Kasahara Hiroyuki、Ikeyama Yoshifumi、Okushima Yoko、Nakajima Keiji、Mimura Tetsuro、Tasaka Masao、Fukaki Hidehiro
    • Journal Title

      New Phytologist

      Volume: 224 Issue: 2 Pages: 749-760

    • DOI

      10.1111/nph.16065

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The RopGEF KARAPPO Is Essential for the Initiation of Vegetative Reproduction in Marchantia polymorpha2019

    • Author(s)
      Hiwatashi T., Goh H., Yasui Y., Koh L.Q., Takami H., Kajikawa M., Kirita H., Kanazawa T., Minamino N., Togawa T., Sato M., Wakazaki M., Yamaguchi K., Shigenobu S., Fukaki H., Mimura T., Toyooka K., Sawa S., Yamato K.T.,Ueda T., Urano D., Kohchi T., Ishizaki K.
    • Journal Title

      Current Biology

      Volume: 29 Issue: 20 Pages: 3525-3531

    • DOI

      10.1016/j.cub.2019.08.071

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] GEMMA CUP-ASSOCIATED MYB1, an ortholog of axillary meristem regulators, is essential in vegetative reproduction in Marchantia polymorpha.2019

    • Author(s)
      Yasui Y, Tsukamoto S, Sugaya T, Nishihama R, Wang Q, Kato H, Yamato KT, Fukaki H, Mimura T, Kubo H, Theres K, Kohchi T, Ishizaki K.
    • Journal Title

      Curr Biol.

      Volume: 29 Issue: 23 Pages: 3987-3995

    • DOI

      10.1016/j.cub.2019.10.004

    • NAID

      120006773950

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Molecular Components of Arabidopsis Intact Vacuoles Clarified with Metabolomic and Proteomic Analyses2018

    • Author(s)
      Miwa Ohnishi, Aya Anegawa, Yuko Sugiyama, Kazuo Harada, Akira Oikawa, Yasumune Nakayama, Fumio Matsuda, Yukiko Nakamura, Ryosuke Sasaki, Chizuko Shichijo Patrick G. Hatcher, Hidehiro Fukaki, Shigehiko Kanaya, Koh Aoki, Mami Yamazaki, Eiichiro Fukusaki, Kazuki Saito, Tetsuro Mimura
    • Journal Title

      Plant and Cell Physiology

      Volume: 69 Pages: 1353-1362

    • DOI

      10.1093/pcp/pcy069

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Cadmium-induced changes in vacuolar aspects of Arabidopsis thaliana.2017

    • Author(s)
      Shanti S. Sharma, Kotaro Yamamoto, Kohei Hamaji, Miwa Ohnishi, Aya Anegawa, Shashi Sharma, Sveta Thakur, Vijay Kumar, Tomohiro Uemura, Akihiko Nakano, Tetsuro Mimura
    • Journal Title

      Plant Physiol Biochem.

      Volume: 114 Pages: 29-37

    • DOI

      10.1016/j.plaphy.2017.02.017

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Plastidial Folate Prevents Starch Biosynthesis Triggered by Sugar Influx into Non-Photosynthetic Plastids of Arabidopsis2017

    • Author(s)
      Hayashi Makoto、Tanaka Mina、Yamamoto Saki、Nakagawa Taro、Kanai Masatake、Anegawa Aya、Ohnishi Miwa、Mimura Tetsuro、Nishimura Mikio
    • Journal Title

      Plant and Cell Physiology

      Volume: 58 Issue: 8 Pages: 1328-1338

    • DOI

      10.1093/pcp/pcx076

    • NAID

      120006473630

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inositol hexakis phosphate is the seasonal phosphorus reservoir in the deciduous woody plant Populus alba L.2017

    • Author(s)
      Kurita, Y., Baba, K., Ohnishi, M., Matsubara, R., Kosuge, K., Anegawa, A., Shichijo, C., Ishizaki, K., Kaneko, Y., Hayashi, M., Suzaki, T., Fukaki, H., Mimura, T.
    • Journal Title

      Plant Cell Physiol.

      Volume: 58 Issue: 9 Pages: 1477-1485

    • DOI

      10.1093/pcp/pcx106

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analyzing the Vacuolar Membrane (Tonoplast) Proteome2017

    • Author(s)
      Ohnishi Miwa、Yoshida Katsuhisa、Mimura Tetsuro
    • Journal Title

      Plant Membrane Proteomics. Methods in Molecular Biology

      Volume: 1696 Pages: 107-116

    • DOI

      10.1007/978-1-4939-7411-5_7

    • ISBN
      9781493974092, 9781493974115
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 2)Cell-specific localization of alkaloids in Catharantus roseus stem tissue measured with Imaging MS and Single cell MS2016

    • Author(s)
      K.Yamamoto, K.Takahashi, H.Mizuno, A.Anegawa, K.Ishizaki, H.Fukaki, M.Ohnishi, M.Yamazaki, T.Masujima, T.Mimura
    • Journal Title

      Proceeding of the National Academy of Sciences of the United States of America

      Volume: 113 Issue: 14 Pages: 3891-3896

    • DOI

      10.1073/pnas.1521959113

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Identification of ice plant (Mesembryanthemum crystallinum L.) microRNAs using RNA-Seq and their putative roles in high salinity responses in seedlings.2016

    • Author(s)
      Chiang Chih-Pin, Yim Won Cheol, Sun Ying-Hsuan, Ohnishi Miwa, Mimura Tetsuro, Cushman John Chandler, Yen Hungchen Emilie
    • Journal Title

      Frontiers in Plant Science

      Volume: 7 Pages: 1143-1143

    • DOI

      10.3389/fpls.2016.01143

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Vacuolar compartmentalization as indispensable component of heavy metal detoxification in plants.2016

    • Author(s)
      Sharma Shanti S, Dietz Karl-Josef, Mimura Tetsuro
    • Journal Title

      Plant Cell & Envrionment

      Volume: 39 Issue: 5 Pages: 1112-1126

    • DOI

      10.1111/pce.12706

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Cell-specific analysis of localization of secondary metabolites in medicinal plant.2020

    • Author(s)
      MIMURA Tetsuro, YAMAMOTO Kotaro, UZAKI Mai, OHNISHI Miwa, MIZUNO Hajime, MASUJIMA Tsutomu, TAKAHASHI Katsutoshi
    • Organizer
      107TH INDIAN SCIENCE CONGRESS, 2020年1月, Bangalore, India.
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ニチニチソウの成長に伴う葉組織二次代謝変動の解析2020

    • Author(s)
      鵜崎真妃,山本浩太郎,栗田悠子,大西美輪,Carlos E. Rodriguez-Lopez,七條千津子,高橋勝利,永野惇,石崎公庸,深城英弘,Sarah E. O’Connor,三村徹郎
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Changes in secondary metabolites related to plant development in Catharanthus roseus.2019

    • Author(s)
      UZAKI Mai, K. YAMAMOTO Kotaro, TAKAHASHI Katsutoshi, OHNISHI Miwa, KURITA Yuko, SHICHIJO Chizuko, NAGANO Atsushi, ISHIZAKI Kimitsune, FUKAKI Hidehiro, MIMURA Tetsuro
    • Organizer
      4th International Meeting on the Biosynthesis, Function and Synthetic Biology of Isoprenoids
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ニチニチソウ乳管細胞・異形細胞の発生,分化,代謝変動の解析2019

    • Author(s)
      鵜崎 真妃,山本 浩太郎,高橋 勝利,大西 美輪,栗田 悠子,七條 千津子,永野 惇,石崎 公庸,深城 英弘,三村 徹郎
    • Organizer
      日本植物学会第83回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Analysis of lipids accumulated in laticifer and idioblast cells in Catharanthus roseus2018

    • Author(s)
      UZAKI Mai, YAMAMOTO Kotaro, TAKAHASHI Katsutoshi, OHNISHI Miwa, ISHIZAKI Kimitsune, FUKAKI Hidehiro, MIMURA Tetsuro
    • Organizer
      International Symposium on Plant Lipids
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ニチニチソウ乳管細胞および異形細胞の分化機構の解明2018

    • Author(s)
      鵜崎真妃、山本浩太郎、高橋勝利、大西美輪、石崎公庸、深城英弘、 三村徹
    • Organizer
      (一社)日本植物生理学会第59回年会(札幌)
    • Related Report
      2017 Annual Research Report
  • [Presentation] 駆け巡るリン2017

    • Author(s)
      三村 徹郎
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Idioblast and laticifer cells play important roles in alkaloid biosynthesis in Catharanthus roseus2017

    • Author(s)
      Kotaro Yamamoto, Katsutoshi Takahashi, Lorenzo Caputi, Hajime Mizuno, Miwa Ohnishi, Kimitsune Ishizaki, Mami Yamazaki, Hidehiro Fukaki, Sarah E O’Connor and Tetsuro Mimura
    • Organizer
      Taiwan Japan Plant Biology 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Idioblast and laticifer cells play important roles in alkaloid biosynthesis in Catharanthus roseus2017

    • Author(s)
      Kotaro Yamamoto, Katsutoshi Takahashi, Lorenzo Caputi, Hajime Mizuno, Miwa Ohnishi, Kimitsune Ishizaki, Mami Yamazaki, Hidehiro Fukaki, Sarah E O’Connor and Tetsuro Mimura
    • Organizer
      Bilateral Closure Symposium of GDRI Integrative Plant Biology Network
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular components of vacuoles and their possible functions in plant cells2016

    • Author(s)
      Tetsuro Mimura; Miwa Ohnishi; Aya Anegawa; Kotaro Yamamoto; Yuko Kurita; Katsuhisa Yoshida
    • Organizer
      第58回日本植物生理学会年会
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ニチニチソウ異形細胞・乳管細胞におけるTIA 代謝機構の解析2016

    • Author(s)
      山本 浩太郎; 大西 美輪; 高橋 勝利; 水野 初; 石崎 公庸; 山崎 真巳; 深城 英弘; 升島 努; 三村 徹郎
    • Organizer
      第58回日本植物生理学会年会
    • Related Report
      2016 Annual Research Report
  • [Presentation] なぜセントポーリアは急激な温度降下に敏感なのか?2016

    • Author(s)
      大西 美輪; 角浜 憲明; 石崎 公庸; 深城 英弘; 鈴木 祥弘; 三村 徹郎
    • Organizer
      第58回日本植物生理学会年会
    • Related Report
      2016 Annual Research Report
  • [Presentation] ニチニチソウ異形細胞におけるTIA 代謝分化機構の解析2016

    • Author(s)
      山本 浩太郎; 高橋 勝利; 水野 初; 大西 美輪; 江崎 剛史; 石崎 公庸; 深城 英弘; 山崎 真巳; 升島 努; 三村 徹郎
    • Organizer
      日本植物学会 第80回大会
    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2022-08-19  

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