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Role of the ciecadian clock gene period in long-term memory

Research Project

Project/Area Number 16H04816
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Animal physiology/Animal behavior
Research InstitutionTokyo Metropolitan University

Principal Investigator

Sakai Takaomi  首都大学東京, 理学研究科, 准教授 (50322730)

Co-Investigator(Kenkyū-buntansha) 武尾 里美  首都大学東京, 理学研究科, 助教 (10642100)
上野 耕平  公益財団法人東京都医学総合研究所, 認知症・高次脳機能研究分野, 副参事研究員 (40332556)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2019: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Keywords長期記憶 / 時計遺伝子 / period / 概日リズムj / 記憶固定化 / 記憶維持 / ショウジョウバエ / Period / LNd / 神経科学 / 脳・神経 / 昆虫 / 行動学 / 生理学
Outline of Final Research Achievements

The circadian clock gene, period (per), was initially identified as a circadian clock gene in Drosophila. We have reported that per is also critically involved in long-term memory (LTM) formation in Drosophila. In this study, we focused on one of the neural clusters in clock neurons (LNd) and studied the role of Per in LTM. First, we established a novel gene expression system to perform the LNd-specific gene expression. Using this system, we identified that Per is required for LTM formation. In addition, we determined that induction of Per lacking two PAS domains (PerΔPAS) in a part of per-positive neurons including LNds impaired LTM, indicating that PerΔPAS also has the dominant-negative effect on LTM formation. PerΔPAS includes the cycle/clock (transcription factor of Per) interaction domain (CCID). Thus, the CCID may be involved in LTM formation.

Academic Significance and Societal Importance of the Research Achievements

我々は時計遺伝子perが長期記憶形成に必須であることを世界で初めて見出した。ハエ脳ではリズム中枢以外の多くのニューロンでperが発現しているにもかかわらず,概日リズム以外の役割が不明である。申請者の発見以降,マウスの相同遺伝子Per1も記憶形成に関与することが報告された。しかし,記憶におけるPERの分子機能は不明であった。本研究で得られた結果は、動物の記憶形成のメカニズムを解明する上で貴重な情報になるのみならず、時計遺伝子の概日リズム以外の機能の解明にも貢献できることに意義がある。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (33 results)

All 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (2 results) Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Open Access: 5 results,  Acknowledgement Compliant: 2 results) Presentation (20 results) (of which Int'l Joint Research: 7 results) Remarks (5 results)

  • [Int'l Joint Research] University of Iowa(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Univ. of Iowa(米国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Environmental Light Is Required for Maintenance of Long-Term Memory in Drosophila2020

    • Author(s)
      Show Inami, Shoma Sato, Shu Kondo, Hiromu Tanimoto, Toshihiro Kitamoto and Takaomi Sakai
    • Journal Title

      Journal of Neuroscience

      Volume: 40 Issue: 7 Pages: 1427-1439

    • DOI

      10.1523/jneurosci.1282-19.2019

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Light is required for proper female mate choice between winged and wingless males in <i>Drosophila</i>2018

    • Author(s)
      Watanabe Kazuki, Suzuki Yuki, Inami Show, Ohashi Hirono, Sakai Takaomi.
    • Journal Title

      Genes & Genetic Systems

      Volume: 93 Issue: 3 Pages: 119-123

    • DOI

      10.1266/ggs.18-00004

    • NAID

      40021700935

    • ISSN
      1341-7568, 1880-5779
    • Year and Date
      2018-06-01
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Synaptic depression induced by postsynaptic cAMP production in the Drosophila mushroom body calyx.2018

    • Author(s)
      Sato S, Ueno K, Saitoe M, Sakai T.
    • Journal Title

      J Physiol

      Volume: 印刷中 Issue: 12 Pages: 2447-2461

    • DOI

      10.1113/jp275799

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Leucokinin signaling regulates hunger-driven reduction of behavioral responses to noxious heat in Drosophila.2018

    • Author(s)
      Ohashi H, Sakai T
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 499 Issue: 2 Pages: 221-226

    • DOI

      10.1016/j.bbrc.2018.03.132

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Modulation of light-driven arousal by LIM-homeodomain transcription factor Apterous in large PDF-positive lateral neurons of the Drosophila brain2016

    • Author(s)
      Shimada N, Inami S, Sato S, Kitamoto S, Sakai T.
    • Journal Title

      Scientific Rep.

      Volume: 6 Issue: 1 Pages: 37255-37255

    • DOI

      10.1038/srep37255

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Mushroom body signaling is required for locomotor activity rhythms in Drosophila2016

    • Author(s)
      Mabuchi I, Shimada N, Sato S, Ienaga K, Inami S, Sakai T.
    • Journal Title

      Neuroscience Res.

      Volume: 111 Pages: 25-25

    • DOI

      10.1016/j.neures.2016.04.005

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Neural circuits required for courtship memory in Drosophila2019

    • Author(s)
      Sato S. and SakaiT.
    • Organizer
      NEUORO2019(第42回日本神経科学大会)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regulation of long-term memoryconsolidation by Cl- influx in Pdf neurons by Drosophila LIM homeodomaintranscription factor, Apterous.2019

    • Author(s)
      Inami S., Sato T.,Sato S., Shimada N., Sakai T.
    • Organizer
      NEUORO2019(第42回日本神経科学大会)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Establishment of virtual courtship learning in Drosophila.2019

    • Author(s)
      Sato T., Sato S.,Sakai T.
    • Organizer
      NEUORO2019(第42回日本神経科学大会)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] period-positive dorsal lateral neurons arerequired for maintenance of long-term memory in Drosophila.2019

    • Author(s)
      Yuki Suzuki, YutoKurata, Takaomi Sakai
    • Organizer
      NEUORO2019(第42回日本神経科学大会)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Photoreceptors involved in light-dependentmaintenance of long-term memory in Drosophila.2019

    • Author(s)
      Hashimoto R.,Inami S., Sakai T.
    • Organizer
      NEUORO2019(第42回日本神経科学大会)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 神経ペプチドPDFを介した光による長期記憶維持システム2019

    • Author(s)
      坂井貴臣
    • Organizer
      第42回日本分子生物学会年会 ワークショップ
    • Related Report
      2019 Annual Research Report
  • [Presentation] Postsynaptic cAMP production predominantly contributes to synaptic depressionin the Drosophila mushroom body calyx.2018

    • Author(s)
      Sato S., Ueno K., Sakai T.
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Regulation of long-term memory processes by Drosophila LIM homeodomaintranscription factor Apteorus in a cofactor-dependent and independent manner.2018

    • Author(s)
      Inami S., Shimada N., Sakai T.
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Innate escapebehavior induced by activation of dopaminergic neurons relevant to punishmentsystem in Drosophila larvae.2018

    • Author(s)
      Sato T., Honjo K., Sakai T.,Furukubo-Tokunaga K.
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Clock neurons involved in period dependent long-term memory in Drosophila.2018

    • Author(s)
      Suzuki Y., Ienaga K., Mabuchi I., Sakai T.
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Light-driven maintenance of long-term memory via the PDF neuropeptide signaling in Drosophila.2017

    • Author(s)
      Inami S., Shimada N., Asano T., Sakai T.
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] The neuropeptide signaling involved in starvation-dependent reduction of heat nociception in Drosophila.2017

    • Author(s)
      Ohashi H., Sakai T.
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Identification of protein domains of the circadian clock protein Period critically involved in Drosophila long-term memory.2017

    • Author(s)
      Mabuchi I., Suzuki Y, Ienaga K., Sakai T.
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Activity-dependent male courtship suppression induced by activation of olfactory projection neurons in Drosophila.2017

    • Author(s)
      Nemoto M., Sakai T.
    • Organizer
      第40回日本神経科学大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] A neuropeptide signaling relulates sarvation-dependent reduction of heat nociception in Drosophila2016

    • Author(s)
      Ohashi H, Sakai T.
    • Organizer
      Annual meeting of Sciety for Neuroscience (Neuroscience 2016)
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2016-11-12
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] cAMP production induced by AL-MB synaptic transmission reduces Ca2+ responses in Drosophila MB2016

    • Author(s)
      Sato S, Sakai T.
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      横浜,神奈川
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] Drosophila LIM homeodomain transcription factor Apteorus regulates acquisition and maintenance of long-term memory.2016

    • Author(s)
      Inami S., Shimada N., Asano T., Sakai T.
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      横浜,神奈川
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] The exploration of neuropeptide involved in starvation-dependent reduction of heat nociception in Drosophila.2016

    • Author(s)
      Ohashi H., Sakai T.
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      横浜,神奈川
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] The circadian clock gene, period, in the dorsal lateral neurons is critically involved in Drosophila long-term memory.2016

    • Author(s)
      Mabuchi I., Ienaga K., Sakai T.
    • Organizer
      第39回日本神経科学大会
    • Place of Presentation
      横浜,神奈川
    • Year and Date
      2016-07-20
    • Related Report
      2016 Annual Research Report
  • [Presentation] Brain is requires for starvation-dependent reduction of heat nociception in Drosophila adults.2016

    • Author(s)
      Ohashi H., Sakai T.
    • Organizer
      JSCPB主催 国際シンポジウム "Environmental Sensing and Animal Behavior"
    • Place of Presentation
      駒場,東京
    • Year and Date
      2016-06-10
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Remarks] 首都大学東京 教員紹

    • URL

      https://www.tmu.ac.jp/stafflist/data/sa/489.html

    • Related Report
      2019 Annual Research Report
  • [Remarks] プレスリリース 【首都大学東京】環境光による記憶維持機構の発見

    • URL

      https://kyodonewsprwire.jp/release/202001105579

    • Related Report
      2019 Annual Research Report
  • [Remarks] ・EurekAlert! News Release 13-Jan-2020

    • URL

      https://www.eurekalert.org/pub_releases/2020-01/sfn-ult010720.php

    • Related Report
      2019 Annual Research Report
  • [Remarks] EurekAlert! News Release 14-Mar-2020

    • URL

      https://www.eurekalert.org/pub_releases/2020-03/tmu-ctm031120.php

    • Related Report
      2019 Annual Research Report
  • [Remarks] 首都大学東京 教員紹介

    • URL

      https://www.tmu.ac.jp/stafflist/data/sa/489.html

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report

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Published: 2016-04-21   Modified: 2022-01-28  

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