| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Outline of Final Research Achievements |
DNA methylation is a chemical modification that acts as a "cellular memory" and plays an important role in cell differentiation and suppression of carcinogenesis. DNA methyltransferase 1 (DNMT1) is a DNA methylation enzyme that ensures accurate inheritance of DNA methylation patterns to daughter DNA during chromosome replication. The function of the E3 ubiquitin ligase UHRF1 is also essential for the localization of DNMT1 at DNA methylation sites, but the molecular mechanism is not yet clear. In this study, we identified PAF15 as a novel DNMT1 interacting protein. We then clarified that UHRF1-dependent ubiquitination of PAF 15 controls the localization of DNMT1 at the methylation sites and ensures faithful inheritance of DNA methylation.
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