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Functional analysis on novel pheromone receptor gene ancV1R, which is shared among most vertebrates

Research Project

Project/Area Number 16H04820
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Evolutionary biology
Research InstitutionTokyo Institute of Technology

Principal Investigator

Nikaido Masato  東京工業大学, 生命理工学院, 准教授 (70432010)

Co-Investigator(Kenkyū-buntansha) 廣田 順二  東京工業大学, バイオ研究基盤支援総合センター, 准教授 (60405339)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
KeywordsV1R / pheromone receptor / vertebrates / evolution / 脊椎動物 / 進化 / フェロモン受容体 / 鋤鼻器官 / ancV1R / 偽遺伝子化 / ノックアウトマウス / フェロモン受容 / フェロモン
Outline of Final Research Achievements

We found a novel pheromone receptor gene belonging to the V1R family, named ancV1R, which is shared among most Osteichthyes (bony vertebrates) from the basal lineage of ray-finned fishes to mammals. Phylogenetic and syntenic analyses of ancV1R using 115 vertebrate genomes revealed that it represents an orthologous gene conserved for >400 million years of vertebrate evolution. Interestingly, the loss of ancV1R in some tetrapods is coincident with the degeneration of the vomeronasal organ (VNO) in higher primates, cetaceans, and some reptiles including birds and crocodilians. In addition, ancV1R is expressed in most mature vomeronasal sensory neurons (VSNs) in contrast with canonical V1Rs, which are sparsely expressed in a manner that is consistent with the “one neuron one receptor” rule. Our results imply that a previously undescribed V1R gene inherited from an ancient Silurian ancestor may have played an important functional role in the evolution of vertebrate VNO.

Academic Significance and Societal Importance of the Research Achievements

本研究では、ほぼ全ての脊椎動物がもつフェロモン受容体遺伝子ancV1Rを発見し、その進化解析をすることで機能を推定した。そもそもフェロモン受容体はそれぞれの種に特異的であり、種間の共通性は低いと考えられていたが、ancV1Rはその予想に反し、驚くべき共通性を保持していた。この研究はこれまでのフェロモン受容体に関するいくつかの常識を覆したため、学術的価値は高い。またancV1Rが、今後の畜産動物の効率的な繁殖管理方法の開発にもつながると期待され社会的な意義も大きいと考えている。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (27 results)

All 2019 2018 2017 2016 Other

All Journal Article (5 results) (of which Peer Reviewed: 3 results) Presentation (19 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results) Remarks (3 results)

  • [Journal Article] A single pheromone receptor gene conserved across 400 million years of vertebrate evolution2018

    • Author(s)
      Suzuki Hikoyu、Nishida Hidefumi、Kondo Hiro、Yoda Ryota、Iwata Tetsuo、Nakayama Kanako、Enomoto Takayuki、Wu Jiaqi、Moriya-Ito Keiko、Miyazaki Masao、Wakabayashi Yoshihiro、Kishida Takushi、Okabe Masataka、Suzuki Yutaka、Ito Takehiko、Hirota Junji、Nikaido Masato
    • Journal Title

      Molecular Biology and Evolution

      Volume: 35 Issue: 12 Pages: 2928-2939

    • DOI

      10.1093/molbev/msy186

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evolution of vomeronasal receptor 1 ( V1R ) genes in the common marmoset ( Callithrix jacchus )2018

    • Author(s)
      Moriya-Ito Keiko、Hayakawa Takashi、Suzuki Hikoyu、Hagino-Yamagishi Kimiko、Nikaido Masato
    • Journal Title

      Gene

      Volume: 642 Pages: 343-353

    • DOI

      10.1016/j.gene.2017.11.048

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Environmental DNA enables detection of terrestrial mammals from forest pond water2017

    • Author(s)
      Masayuki Ushio, Hisato Fukuda, Toshiki Inoue, Kobayashi Makoto, Osamu Kishida, Keiichi Sato, Koichi Murata, Masato Nikaido, Tetsuya Sado, Yukuto Sato, Masamichi Takeshita, Wataru Iwasaki, Hiroki Yamanaka, Michio Kondoh, and Masaki Miya
    • Journal Title

      Molecular Ecology Resources

      Volume: 17(6):e Issue: 6 Pages: 63-75

    • DOI

      10.1111/1755-0998.12690

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 脊椎動物のV1R型フェロモン受容体遺伝子ファミリーの進化2016

    • Author(s)
      二階堂雅人
    • Journal Title

      AROMARESEARCH

      Volume: 17 Pages: 230-235

    • Related Report
      2016 Annual Research Report
  • [Journal Article] 生きた化石シーラカンス2016

    • Author(s)
      二階堂雅人
    • Journal Title

      『Re』建築保全センター

      Volume: 189 Pages: 58-59

    • Related Report
      2016 Annual Research Report
  • [Presentation] 哺乳類ゲノム解析から探るancV1Rへの進化圧への緩みと鋤鼻器官の退化2019

    • Author(s)
      二階堂雅人
    • Organizer
      第6回 ケモビ研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 古代魚におけるフェロモン受容器官の探索2019

    • Author(s)
      佐久間敦丈、二階堂雅人
    • Organizer
      第1回遺伝学会分科会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 広範な脊椎動物に共通に存在するフェロモン受容体遺伝子の進化解析2018

    • Author(s)
      依田涼太、鈴木彦有、岩田哲郎、廣田順二、二階堂雅人
    • Organizer
      日本進化学会第20回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 脊椎動物ゲノム探索から明らかとなったフェロモン受容体 V1Rの多様性の起源2018

    • Author(s)
      依田涼太、二階堂雅人
    • Organizer
      日本進化学会第20回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 脊椎動物の4億年以上に渡る進化の過程で保持されてきたフェロモン受容体2018

    • Author(s)
      鈴木彦有,佐久間敦丈,依田涼太,岡部正隆,伊藤武彦,廣田順二, 二階堂雅人
    • Organizer
      第8回 Tokyo Vertebrate Morphology Meeting
    • Related Report
      2018 Annual Research Report
  • [Presentation] A single pheromone receptor gene shared among most bony vertebrates2018

    • Author(s)
      Nikaido, M., Suzuki, H., Ito, T., Hirota, J.
    • Organizer
      SMBE 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 下位条鰭類ゲノム探索で明かされた脊椎動物V1Rの多様性2018

    • Author(s)
      二階堂雅人
    • Organizer
      第5回 ケモビ研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 硬骨魚類における苦味受容体遺伝子の多様性と共通性2018

    • Author(s)
      畑島諒、二階堂雅人
    • Organizer
      第5回 ケモビ研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 鋤鼻器官の退化に伴うancV1R遺伝子に働く自然選択圧の緩みの検証2018

    • Author(s)
      張子聡、二階堂雅人
    • Organizer
      第5回 ケモビ研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Evolution of V1R pheromone receptor genes in vertebrates: its diversity and generality2017

    • Author(s)
      Nikaido, M
    • Organizer
      5th Advance in Neuroinformatics
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 広範な脊椎動物に共通な新規フェロモン受容体の進化2017

    • Author(s)
      二階堂雅人
    • Organizer
      日本味と匂学会第51回大会・嗅覚シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 広範な脊椎動物が共有する新規フェロモン受容体の進化と機能2017

    • Author(s)
      鈴木彦有、依田涼太、岩田哲郎、廣田順二、二階堂雅人
    • Organizer
      日本動物学会第88回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 古代魚におけるancV1R 遺伝子の発現解析2017

    • Author(s)
      鈴木英里、二階堂雅人
    • Organizer
      日本動物学会第88回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 広範な脊椎動物が共有する新規フェロモン受容体の進化と機能2017

    • Author(s)
      鈴木彦有、岩田哲郎、依田涼太、廣田順二、二階堂雅人
    • Organizer
      日本進化学会第19回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 全ゲノム重複に伴う魚類OMPの進化解析2017

    • Author(s)
      小郷卓博、鈴木彦有、二階堂雅人
    • Organizer
      日本進化学会第19回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ほぼ全ての脊椎動物が共有するフェロモン受容体ancV1Rの進化解析2017

    • Author(s)
      二階堂雅人
    • Organizer
      第4回ケモビ研究会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 広範な脊椎動物に共有される新規V1R受容体遺伝子2016

    • Author(s)
      鈴木彦有、依田涼太、中山叶子、岩田哲郎、村田健、東原和成、廣田順二、二階堂雅人
    • Organizer
      日本進化学会第18回大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Multiple episodic evolution events in V1R receptor genes of East-African cichlids2016

    • Author(s)
      Nikaido, M, Suzuki H, Satta Y, Mzighani SI, Sturmbauer C, Hagino-Yamagishi K, Okada N.
    • Organizer
      17th International Symposium on Olfaction and Taste
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Evolution of V1R pheromone receptor genes in vertebrates: its diversity and generality2016

    • Author(s)
      Nikaido M
    • Organizer
      The 87th Annual Meeting of the Zoological Society of Japan
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Remarks] 二階堂研究室

    • URL

      http://www.nikaido.bio.titech.ac.jp

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
  • [Remarks] ほぼ全ての脊椎動物に共通するフェロモン受容体を発見

    • URL

      https://www.titech.ac.jp/news/2018/042562.html

    • Related Report
      2018 Annual Research Report
  • [Remarks] 二階堂研究室

    • URL

      http://www.nikaido.bio.titech.ac.jp/index.html

    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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