Studies on ecotypic speciation mechanism in plants: Comparative genomic analysis in Solidago virgaurea group

• Project/Area Number: 16H04827
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biodiversity/Systematics
• Research Institution: The University of Tokyo
• Principal Investigator: Ito Motomi 東京大学, 大学院総合文化研究科, 教授 (00193524)
• Co-Investigator(Kenkyū-buntansha): 久保田 渉誠 東京大学, 大学院総合文化研究科, 助教 (10771701) ; 阪口 翔太 京都大学, 地球環境学堂, 助教 (50726809) ; 牧 雅之 東北大学, 学術資源研究公開センター, 教授 (60263985)
• Research Collaborator: Ishikawa Naoko ; Nishino Takako
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2017: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2016: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
• Keywords: 生態型分化 / アキノキリンソウ / ゲノム解析 / 種分化

Outline of Final Research Achievements

We have read total genome sequences for the purpose to clarify the mechanism of speciation for the Solidago which has undergone large-scale ecological speciation in the Japanese archipelago. Then, resequencing was performed for each ecotype, and mapping and mutation detection analyses were performed. As a result, it was revealed that the genetic differentiation of ion transporters such as Mg2 +, K +, NO3- and heavy metals, and flowering related genes is high between populations of serpentine soil and non-serpentine soil. On the other hand, in the comparison of alpine type and forest floor type, the degree of differentiation of flowering genes is low, and candidate genes found from serpentine types may not be involved in early-flowering of alpine type.

Academic Significance and Societal Importance of the Research Achievements

種分化研究において、環境への適応の結果、並行的に同じような分化が起きる生態的種分化は、多くの研究例は系統解析に留まっていた。大規模な生態的種分化が起きている植物において、生態学的、遺伝学的、系統学的アプローチでその全容をゲノムレベルで解析ができ、非モデル植物群において種分化現象からその遺伝的バックグラウンドまで解明できたことは大きな学術的成果であ理、今後の本分野の研究の先駆けとなるものである。

Research Products

• [Journal Article] Rapid development and characterization of EST SSR markers for the honey locust seed beetle, Megabruchidius dorsalis (Coleoptera: Bruchidae), using de novo transcriptome analysis based on next generation sequencing. (2019)
  • Author(s): Kako Ohbayashi, Naoko Ishikawa, Yoshikuni Hodoki, Yasukazu Okada,, Shin ichi Nakano, Motomi Ito, Masakazu Shimada
  • Journal Title: Applied Entomology and Zoology Volume: 54 Pages: 141-145
  • NAID: 40021825482
  • Peer Reviewed
• [Journal Article] Genetic diversity and population demography of Geranium soboliferum Kom var. kiusianum: A glacial relict plant in the wetlands of Japan, (2019)
  • Author(s): Kurata S, Sakaguchi S and Ito M.
  • Journal Title: Conservation Genetics Volume: 20 Pages: 431-445
  • Peer Reviewed
• [Journal Article] Phylogeographic testing of alternative histories of single-origin versus parallel evolution of early flowering serpentine populations of Picris hieracioides L. (Asteraceae) in Japan (2018)
  • Author(s): Sakaguchi Shota、Horie Kenji、Kimura Takuma、Nagano Atsushi J.、Isagi Yuji、Ito Motomi
  • Journal Title: Ecological Research Volume: 33 Issue: 3 Pages: 537-547
  • DOI: 10.1007/s11284-017-1536-2
  • Peer Reviewed
• [Journal Article] Phylogeographic analysis of the East Asian goldenrod (Solidago virgaurea complex, Asteraceae) reveals hidden ecological diversification with recurrent formation of ecotypes (2017)
  • Author(s): Sakaguchi Shota、Kimura Takuma、Kyan Ryuta、Maki Masayuki、Nishino Takako、Ishikawa Naoko、Nagano Atsushi J、Honjo Mie N、Yasugi Masaki、Kudoh Hiroshi、Li Pan、Choi Hyeok Jae、Chernyagina Olga A、Ito Motomi
  • Journal Title: Annals of Botany Volume: 121 Issue: 3 Pages: 489-500
  • DOI: 10.1093/aob/mcx182
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Simultaneous evaluation of the effects of geographic, environmental and temporal isolation in ecotypic populations of Solidago virgaurea. (2017)
  • Author(s): Shota Sakaguchi, Naoko Ishikawa, Atsushi J. Nagano, Masaki Yasugi, Hiroshi Kudoh, Motomi Ito
  • Journal Title: New Phytologist Volume: 216 Issue: 4 Pages: 1268-1280
  • DOI: 10.1111/nph.14744
  • Peer Reviewed
• [Presentation] 寒い高山と暑い蛇紋岩地で平行進化した早咲きアキノキリンソウのゲノム解析 (2019)
  • Author(s): 阪口翔太, 堀江健二, 重信秀治, 山口勝司, 長谷部光泰, 久保田渉誠, 石川直子, 瀬戸口 浩彰, 伊藤元己
  • Organizer: 第66回大会日本生態学会、神戸
• [Presentation] Genetic uniqueness and population demography of relict plants distributed in wetlands of semi-natural grassland (2018)
  • Author(s): Kurata S, Sakaguchi S, Ito M.
  • Organizer: he 2nd International Academic Conference on the Formation Mechanism of Plant Diversity and Conservation of Endangered Plants in East Asia, Kyoto
  • Int'l Joint Research
• [Presentation] サワシロギクの蛇紋岩適応における微生物の作用と土壌細菌の菌叢解析 (2018)
  • Author(s): 真鍋遼、福島慶太郎、石川直子、阪口翔太、伊藤元己、西野貴子
  • Organizer: 日本植物学会第82回大会、広島市
• [Presentation] 開花期が異なる土壌エコタイプ間での遺伝子流動：キク科2属での検証 (2018)
  • Author(s): 阪口翔太, 堀江健, 石川直子, 永野惇, 八杉公基, 工藤洋, 成田あゆ, 井鷺裕司, 伊藤元己
  • Organizer: 日本生態学会第65回全国大会 (2018年3月14-18日、札幌市白石区)
• [Presentation] 早期開花性を獲得した蛇紋岩型アキノキリンソウの集団分化と局所適応 (2018)
  • Author(s): 阪口翔太, 堀江健二, 石川直子, 永野惇, 本庄三恵, 工藤洋, 福島慶太郎, 成田あゆ, 山崎理正, 井鷺裕司, 伊藤元己
  • Organizer: 日本生態学会第64回全国大会（2017年3月14-18日, 東京都新宿区）