• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Intraspecific differentiation reinforced by flowering time differentiation as local adaptation caused by the genotype polymorphisms

Research Project

Project/Area Number 16H04831
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biodiversity/Systematics
Research InstitutionKyoto University

Principal Investigator

Setoguchi Hiroaki  京都大学, 地球環境学堂, 教授 (70206647)

Co-Investigator(Kenkyū-buntansha) 川口 正代司  基礎生物学研究所, 共生システム研究部門, 教授 (30260508)
Research Collaborator WAKABAYASHI tomomi  
HAN Chingxiang  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2018: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Keywords開花 / 繁殖成長 / 栄養成長 / 生殖前隔離 / 生育環境 / ミヤコグサ / ハマダイコン / 環境相互作用 / 生殖隔離 / ゲノムワイド関連解析 / 植物 / 進化 / 適応 / 開花関連遺伝子 / 春化処理 / 種内分化 / 日長 / 春化
Outline of Final Research Achievements

Flowering time differentiation within a plant species can cause pre-zygotic isolation and sculpture the intraspecific differentiation. In this financial year 2018, we cultivated 132 wild lines of Lotus japonicas (Fabaceae) in Okinawa and conducted Genome Wide Association Analysis (GWAS) and identified plural candidate genes as "outlier loci" that would associated with determining early or late flowering determination. In addition, we also investigated local differentiation of vernalization system using wild radish (Raphanus sativus: Brassicaceae). qRT-PCR revealed that floral initiation in the northern lineage was primarily controlled by FLC-A, while COL5-A initiated flowering in the southern accessions despite the presence of high FLC-A transcript levels.

Academic Significance and Societal Importance of the Research Achievements

日本列島は北海道から沖縄まで、南北方向に長い島国です。北海道は亜寒帯、沖縄は亜熱帯の気候です。そのために、広い範囲に分布する植物は、地域の環境に適応して花を咲かせる時期を決めています。この研究では、ミヤコグサという植物が日長などを感じる様々な遺伝子の相互作用で、ハマダイコンという植物では冬の寒さを一定期間にわたって経験するか(春化)ということに関わる2つの遺伝子の相互作用で適応していることがわかりました。このように、1種の植物のなかでも、日本列島の地域ごとに適切な時期に開花を迎えるためのしくみがわかってきました。このような仕組みは、農作物の収量を最大にしたり、野生植物の保護に役立つものです。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (22 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (3 results) Journal Article (11 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 11 results,  Open Access: 9 results,  Acknowledgement Compliant: 4 results) Presentation (7 results) (of which Invited: 1 results) Remarks (1 results)

  • [Int'l Joint Research] オーフス大学(デンマーク)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] オーフス大学(デンマーク)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] オーフス大学(デンマーク)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Approximate Bayesian computation analysis of EST associated microsatellites indicates that the broadleaved evergreen tree Castanopsis sieboldii survived the Last Glacial Maximum in multiple refugia in Japan2019

    • Author(s)
      Kyoko AOKI, Ichiro Tamaki, Katsuhiro Nakao, Saneyoshi Ueno, Takashi Kamijo, Hiroaki Setoguchi, Noriaki Murakami, Makoto Kato, Yoshihiko Tsumura
    • Journal Title

      Heredity

      Volume: 122 Issue: 3 Pages: 326-340

    • DOI

      10.1038/s41437-018-0123-9

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Exploring genetic diversity and signatures of horizontal gene transfer in nodule bacteria associated with Lotus japonicus in natural environments2019

    • Author(s)
      Bamba, M., Aoki, S., Kajita, T., Setoguchi H., Watano, Y., Sato, S., and Tsuchimatsu, T.
    • Journal Title

      Molecular Plant-Microbe Interactions

      Volume: 印刷中 Issue: 9 Pages: 1110-1120

    • DOI

      10.1094/mpmi-02-19-0039-r

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Postglacial East Asian origin of the alpine shrub Phyllodoce aleutica (Ericaceae) in Beringia.2018

    • Author(s)
      Ikeda H, Yakubov V, Barkalov V, Setoguchi H.
    • Journal Title

      Journal of Biogeography

      Volume: in press Issue: 6 Pages: 1260-1274

    • DOI

      10.1111/jbi.13230

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Genetic structure of the clonal herb Tanakaea radicans (Saxifragaceae) at multiple spatial scales, revealed by nuclear and mitochondrial microsatellite markers2018

    • Author(s)
      Sakaguchii, S., Takahashi, D., Setoguchi, H., Isagi, Y.
    • Journal Title

      Plant Species Biology

      Volume: 33 Issue: 1 Pages: 81-87

    • DOI

      10.1111/1442-1984.12196

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genetic diversity of Saxifraga acerifolia and S. fortune based on nuclear and chloroplast microsatellite markers2018

    • Author(s)
      Magota, K., Sakaguchi, S., Akai, K., Isagi, Y., Setoguchi, H.
    • Journal Title

      Bulletin of the National Science Museum of Nature and Science, Series B.

      Volume: 44 Pages: 85-96

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparative chloroplast genomics of series Sakawanum in genus Asarum (Aristolochiaceae) to develop single nucleotide polymorphisms (SNPs) and simple sequence repeat (SSR) markers2018

    • Author(s)
      Takahashi Daiki、Sakaguchi Shota、Isagi Yuji、Setoguchi Hiroaki
    • Journal Title

      Journal of Forest Research

      Volume: 23 Issue: 6 Pages: 387-392

    • DOI

      10.1080/13416979.2018.1518649

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Tight species cohesion among insular wild gingers (Asarum spp. Aristrochiaceae) on continental islands: highly differentiated floral characteristics versus undifferentiated genotypes.2017

    • Author(s)
      Matsuda, J., Maeda, Y., Nagasawa, J., Setoguchi, H.
    • Journal Title

      PLOS ONE

      Volume: 12(3) Issue: 3 Pages: e0173489-e0173489

    • DOI

      10.1371/journal.pone.0173489

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Conservation of endangered plant Primula on limestone mountain Mt. Buko, Japan2017

    • Author(s)
      Yamamoto, M., Setoguchi, H., Kurata, K.
    • Journal Title

      Conservation Genetics

      Volume: 印刷中 Issue: 5 Pages: 1141-1150

    • DOI

      10.1007/s10592-017-0966-2

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Development and characterization of EST-SSR markers in Asarum sakawanum var. stellatum and cross-amplification in related species2017

    • Author(s)
      Takahashi, D., Sakaguchi, S., Setoguchi, H.
    • Journal Title

      Plant Species Biology

      Volume: 印刷中 Issue: 3 Pages: 256-260

    • DOI

      10.1111/1442-1984.12156

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Lineage isolation in the face of active gene flow in the coastal plant wild radish is reinforced by differentiated vernalisation responses2016

    • Author(s)
      Han, Q., Higashi, H., Mitsui, Y., Setoguchi, H.
    • Journal Title

      BMC Evolutionary Biology

      Volume: in press Issue: 1 Pages: 84-84

    • DOI

      10.1186/s12862-016-0655-7

    • NAID

      120005947144

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Geographic variation in leaf trichome of wild radish (Raphanus sativus L. var. raphanistroides Makino) in Japan2016

    • Author(s)
      Han, Q., Sakaguchi, S., Setoguchi, H.
    • Journal Title

      Journal of Biogeography and Taxonomy

      Volume: 64 Pages: 17-23

    • NAID

      120006557750

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] ミヤコグサ国内野生系統が示す開花時期の大きな違いとその遺伝基盤~全ゲノム関連解析による責任遺伝子の検出~2018

    • Author(s)
      瀬戸口浩彰、若林智美
    • Organizer
      日本植物学会第82回大会 シンポジウム「植物系 NBRP リソースとその活用研究最前線」
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Genome-wide association study for differences of flowering time variation under two conditions in Lotus japonicus in Japan2018

    • Author(s)
      若林智美、Stig U.Andersen、佐藤修正、川口正代司、瀬戸口浩彰
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Lineage isolation maintained by natural selection despite ongoing gene flow in Japanese wild radish.2017

    • Author(s)
      韓慶香,東広之,阪口翔太,三井裕樹,瀬戸口浩彰
    • Organizer
      日本植物分類学会第16回大会
    • Place of Presentation
      京都大学
    • Year and Date
      2017-03-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] Detecting loci contributing local adaptation through flowering time in Lotus japonicus in Japan.2017

    • Author(s)
      若林智美,田中幸子,Stig U. Andersen,佐藤修正,川口正代司,瀬戸口浩彰
    • Organizer
      日本植物分類学会第16回大会
    • Place of Presentation
      京都大学
    • Year and Date
      2017-03-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] Detecting loci contributing local adaptation through flowering time in Lotus japonicus in Japan2017

    • Author(s)
      若林智美,田中幸子,Stig U. Andersen,佐藤修正,川口正代司,瀬戸口浩彰
    • Organizer
      日本植物分類学会第16回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Genome-wide association study for differences of flowering time variation under two conditions in Lotus japonicus in Japan2017

    • Author(s)
      若林智美、Stig U.Andersen、佐藤修正、川口正代司、瀬戸口浩彰
    • Organizer
      全ゲノム関連解析によるミヤコグサの地域適応遺伝子の探索. 日本生態学会 第64回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ミヤコグサの開花時期の多型を生む遺伝的要因の探索 ~全ゲノム情報を利用して.2016

    • Author(s)
      若林智美,Andersen, SU, 佐藤修正,川口正代司,瀬戸口浩彰
    • Organizer
      日本植物学会第80回大会
    • Place of Presentation
      沖縄県宜野湾市,沖縄コンベンションセンター
    • Year and Date
      2016-09-08
    • Related Report
      2016 Annual Research Report
  • [Remarks] 京都大学大学院人間・環境学研究科 瀬戸口浩彰研究室

    • URL

      http://setolab.h.kyoto-u.ac.jp

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2021-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi