Seaweed vegetations around Japan during LGM and their recovery focusing the coastal environment of the Sea of Japan

• Project/Area Number: 16H04832
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biodiversity/Systematics
• Research Institution: Kobe University
• Principal Investigator: Kawai Hiroshi 神戸大学, 内海域環境教育研究センター, 教授 (30161269)
• Co-Investigator(Kenkyū-buntansha): 鈴木 雅大 神戸大学, 内海域環境教育研究センター, 特命助教 (30637088) ; 羽生田 岳昭 神戸大学, 内海域環境教育研究センター, 助教 (40379334) ; 廣瀬 孝太郎 早稲田大学, 理工学術院, 助教 (60596427) ; 岡崎 裕典 九州大学, 理学研究院, 准教授 (80426288)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2016: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
• Keywords: 海藻類 / 系統地理 / 日本海 / 最終最大氷期 / レフュジア / 海藻 / 最大氷期 / 遺伝子解析

Outline of Final Research Achievements

In order to elucidate the phylogeography of seaweeds around Japan during and after Last Glacial Maximum, molecular phylogeny and analyses of the genetic diversity in local populations of ten selected brown and red algae were examined using DNA sequences of cox1, cox3，nad3-16S rDNA，tatC-tLeu genes. In many species the population in Korea and western Japan and those in Pacific eastern Japan showed were genetically close, and there were discontinuities around Kii Peninsula. This suggested that two independent refugia existed during the LGM. Some species showed great genetic diversities along the Sea of Japan coast suggesting that the Tsugaru Current has continued to enter into the area even during the LGM. This notion was further supported by the oxygen isotope ratio analyses of the planktonic foraminiferal shells.

Academic Significance and Societal Importance of the Research Achievements

氷河期などの大規模な海況の変化は，海洋生物の分布に大きな影響を与え，現在の海洋生物の分布も直接的・間接的にその影響を受けている。なかでも日本海は最大氷河期には淡水化し，ほとんどの海藻類が死滅したとされるが，化石がほとんど残らないためその詳細については不明であった。本研究は現在の海藻類の各地域集団の遺伝的多様性を解析することで，最終最大氷期の日本海の海況や，寒冷化した海で海藻類の集団が生き残った場所であるレフュジアの位置を推定することと目指したものである。本研究の結果，最終最大氷期には東日本と西日本の２カ所に独立したレフュジアがあり，また日本海でもある程度の海藻が生残していたことが示された。

Research Products

• [Int'l Joint Research] Oregon State University(米国)
• [Int'l Joint Research] Institute of Oceanology(中国)
• [Int'l Joint Research] University of A Coruna(スペイン)
• [Journal Article] Tinocladia sanrikuensis sp. nov. (Ectocarpales s.l., Phaeophyceae) from Japan (2019)
  • Author(s): Hanyuda Takeaki、Takeuchi Kazusa、Kawai Hiroshi
  • Journal Title: Phycological Research Volume: 67 Issue: 3 Pages: 1-7
  • DOI: 10.1111/pre.12367
  • Peer Reviewed
• [Journal Article] Genetic examination of the type specimen of Ulva australis suggests that it was introduced to Australia. (2018)
  • Author(s): Hanyuda T. and Kawai H.
  • Journal Title: Phycol. Res. Volume: 66 Issue: 3 Pages: 238-241
  • DOI: 10.1111/pre.12222
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Possible origins of planktonic copepods, Pseudodiaptomus marinus (Crustacea: Copepoda: Calanoida), introduced from East Asia to the San Francisco Estuary based on a molecular analysis (2018)
  • Author(s): Ohtsuka, S., Shimono, T., Hanyuda, T., Shang, X., Huang, C., Soh, H.Y., Kimmerer, W., Kawai, H., Itoh, H., Ishimaru, T., Tomikawa, K.
  • Journal Title: Aquatic Invasions Volume: 13 Issue: 2 Pages: 221-230
  • DOI: 10.3391/ai.2018.13.2.04
  • NAID: 120006491586
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular phylogeny of the benthic dinoflagellate Cabra matta (Dinophyceae) from Okinawa, Japan (2018)
  • Author(s): Yamaguchi Aika、Wakeman Kevin C.、Hoppenrath Mona、Horiguchi Takeo、Kawai Hiroshi
  • Journal Title: Phycologia Volume: 57 Issue: 6 Pages: 630-640
  • DOI: 10.2216/18-7.1
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The invasion threat of benthic marine algae arriving on Japanese tsunami marine debris (JTMD) in Oregon and Washignton, USA. (2018)
  • Author(s): Hansen G.I., Hanyuda T. and Kawai H.
  • Journal Title: Phycologica Volume: 57 Issue: 6 Pages: 641-658
  • DOI: 10.2216/18-58.1
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Ecological shifts due to anthropogenic activities in the coastal seas of the Seto Inland Sea, Japan, since the 20th century (2018)
  • Author(s): Irizuki Toshiaki、Hirose Kotaro、Ueda Yukari、Fujihara Yuki、Ishiga Hiroaki、Seto Koji
  • Journal Title: Marine Pollution Bulletin Volume: 127 Pages: 637-653
  • DOI: 10.1016/j.marpolbul.2017.12.050
  • Peer Reviewed
• [Journal Article] Two new species of Padina (Dictyotales, Phaeophyceae) from southern Japan, P. ogasawaraensis sp. nov. and P. reniformis sp. nov., based on morphology and molecular markers (2018)
  • Author(s): Ni-Ni-Win、Hanyuda Takeaki、Kato Aki、Kawai Hiroshi
  • Journal Title: Phycologia Volume: 57 Issue: 1 Pages: 20-31
  • DOI: 10.2216/17-25.1
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Possible origins of planktonic copepods, Pseudodiaptomus marinus (Crustacea: Copepoda: Calanoida), introduced from East Asia to the San Francisco Estuary based on a molecular analysis (2018)
  • Author(s): Ohtsuka, S., Shimono, T., Hanyuda, T., Shang, H., Huang, C., Soh, X.Y., Kimmerer, M., Kawai, H., Itoh, H., Ishimaru, T., Tomikawa, K.
  • Journal Title: Aquatic Invasions Volume: 13
  • NAID: 120006491586
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genetic examination of the type specimen of Ulva australis suggests that it was introduced to Australia (2018)
  • Author(s): Hanyuda, T., Kawai, H.
  • Journal Title: Phycol. Res. Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Molecular phylogeny of the benthic dinoflagellate Cabra matta (Dinophyceae) from Okinawa, Japan (2018)
  • Author(s): Yamaguchi, A., Wakeman, K.C., Hoppenrath, M., Horiguchi, T., Kawai, H.
  • Journal Title: Phycologia Volume: 印刷中
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Carbon dioxide mitigation potential of seaweed aquaculture beds (SABs) (2017)
  • Author(s): Calvyn F.A. Sondak, C.F.A., Ang, P.O.Jr., Beadall, J., Bellgrove, A., Boo, S.M., Gerung, G.S., Hepburn, C.D., Hong, D.D., Hu, Z., Kawai, H., Lim, P.-E., Largo, D., Lee, J.A., Mayakun, J., Nelson, W.A., Oak, J.H., Phang, S.M., Sahoo, D., Peerapornpis, Y., Yang, Y., Chung, I.K.
  • Journal Title: J. Appl. Phycol. Volume: 29 Issue: 5 Pages: 2363-2373
  • DOI: 10.1007/s10811-016-1022-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genome-wide computational analysis of the secretome of brown algae (Phaeophyceae) (2017)
  • Author(s): Terauchi Makoto、Yamagishi Takahiro、Hanyuda Takeaki、Kawai Hiroshi
  • Journal Title: Marine Genomics Volume: 32 Pages: 49-59
  • DOI: 10.1016/j.margen.2016.12.002
  • Peer Reviewed
• [Journal Article] Two new species of Mesospora (Ralfsiales, Phaeophyceae) from the subtropical Indo-Pacific region (2017)
  • Author(s): Poong, S.-W, Lim P.-E., Phang, S.-M., Sunarpi, H., West, J.A., Miller, K.A., Nelson, W.A., Kawai, H.
  • Journal Title: Phycologia Volume: 56 Issue: 5 Pages: 487-498
  • DOI: 10.2216/16-142.1
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Genetic identification of macroalgal species on Japanese tsunami marine debris and genetic comparisons with their wild populations (2017)
  • Author(s): Hanyuda, T., Hansen, G.I., Kawai, H.
  • Journal Title: Mar. Poll. Bull. Volume: 印刷中 Pages: 74-81
  • DOI: 10.1016/j.marpolbul.2017.06.053
  • NAID: 120006501221
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Millennial-scale northern Hemisphere Atlantic-Pacific climate teleconnections in the earliest Middle Pleistocene (2017)
  • Author(s): Hyodo Masayuki、Bradak Balazs、Okada Makoto、Katoh Shigehiro、Kitaba Ikuko、Dettman David L.、Hayashi Hiroki、Kumazawa Koyo、Hirose Kotaro、Kazaoka Osamu、Shikoku Kizuku、Kitamura Akihisa
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 10036-10036
  • DOI: 10.1038/s41598-017-10552-2
  • NAID: 120006352251
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular phylogeny of the benthic dinoflagellate genus Amphidiniopsis and its relationships with the family Protoperidiniaceae (2017)
  • Author(s): Yamaguchi, A., Yoshimatsu, S., Hoppenrath, M., Wakeman, K.C., Kawai, H.
  • Journal Title: Protist Volume: 印刷中
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Taxonomic revision of the Agaraceae with a description of Neoagarum gen. nov. and reinstatement of Thalassiophyllum (2017)
  • Author(s): Kawai, H., Hanyuda, T., Gao, X., Terauchi, M., Miyata, M., Lindstrom, S.C., Klochkova, N.G., Miller, K.A.
  • Journal Title: J. Phycol. Volume: 印刷中
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Phylogeographic analysis of the brown alga Cutleria multifida (Tilopteridales, Phaeophyceae) suggests a complicated introduction history (2016)
  • Author(s): Kawai H., Kogishi K., Hanyuda T., Arai S., Gurgel C.F., Nelson W., Meinesz A., Tsiamis K. and Peters A.F.
  • Journal Title: Phycological Research Volume: 64 Issue: 1 Pages: 3-10
  • DOI: 10.1111/pre.12113
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Taxonomic revision of Papenfussiella species (Chordariaceae, Phaeophyceae) in the Northern Hemisphere (2016)
  • Author(s): Kawai, H., Miyoshi, K., Hanyuda, T.
  • Journal Title: Phycologia Volume: 55 Issue: 3 Pages: 308-317
  • DOI: 10.2216/15-139.1
  • Peer Reviewed
• [Journal Article] Phylogenetic analysis of mtDNA markers for Asparagopsis taxiformis (Bonemaisoniales, Rhodophyta) provides novel insights into their phylogeography (2016)
  • Author(s): Kurihara, A., Horiguchi, H., Hanyuda, T., Kawai, H.
  • Journal Title: Phycol. Res. Volume: 64 Issue: 2 Pages: 95-101
  • DOI: 10.1111/pre.12126
  • Peer Reviewed
• [Journal Article] Genetic diversity and biogeography of native and introduced populations of Ulva pertusa (Ulvales, Chlorophyta) (2016)
  • Author(s): Hanyuda, T., Heesch, S., Nelson, W., Sutherland, J., Arai, S., Boo, S.M., Kawai, H.
  • Journal Title: Phycol. Res. Volume: 64 Issue: 2 Pages: 102-109
  • DOI: 10.1111/pre.12123
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Molecular phylogeny of Zeacarpa (Ralfsiales, Phaeophyceae) proposing a new family Zeacarpaceae and its transfer to Nemodermatales (2016)
  • Author(s): Kawai, H., Hanyuda, T., Bolton, J., Anderson, R.
  • Journal Title: J. Phycol. Volume: 52 Issue: 4 Pages: 682-686
  • DOI: 10.1111/jpy.12419
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Cladosiphon takenoensis sp. nov. (Ectocarpales s.l., Phaeophyceae) from Japan (2016)
  • Author(s): Kawai, H., Hanyuda, T., Kim, S.H., Ichikawa, Y., Uwai, S., Peters, A.F.
  • Journal Title: Phycol. Res. Volume: 64 Issue: 4 Pages: 212-218
  • DOI: 10.1111/pre.12140
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Next-generation sequencing of an 88-year-old specimen of the poorly known species Liagora japonica (Nemaliales, Rhodophyta) supports the recognition of Otohimella gen. nov. (2016)
  • Author(s): Suzuki, M., Segawa, T. Mori, H., Akiyoshi, A., Ootsuki, R., Kurihara, A., Sakayama, H., Kitayama, T., Abe, T., Kogame, K., Kawai, H., Nozaki, H.
  • Journal Title: PLOS ONE Volume: 11 Issue: 7 Pages: e0158944-e0158944
  • DOI: 10.1371/journal.pone.0158944
  • NAID: 120005844143
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] A quest for the origin of laminarian kelps (2019)
  • Author(s): Kawai, H.
  • Organizer: British Phycological Society 67th Annual Meeting
  • Int'l Joint Research / Invited
• [Presentation] Taxonomic revision of Eudesme (Ectocarpales s.l., Phaeophyceae). (2018)
  • Author(s): Kawai, H., Hanyuda, T., Sun, Z.M., Barbara, I., Peters, A.F.
  • Organizer: British Phycological Society Winter Meeting 2018
  • Int'l Joint Research
• [Presentation] Taxonomic revision of Japanese Sauvageaugloia species (Ectocarpales s.l., Phaeophyceae) (2018)
  • Author(s): Kawai, H., Hanyuda, T., Shibata, K., Kamiya, M., Peters, A.F.
  • Organizer: 72nd Annual Phycological Society of America Meeting
  • Int'l Joint Research
• [Presentation] Ten years’ monitoring of subtidal algal beds of Hyogo Prefecture by the “Monitoring Sites 1000 Moba” Project (2018)
  • Author(s): Kawai, H., Abe, M., Kamiya, M., Kurashima, A., Shimabukuro, H., Murase, N., Yoshida, G., Terada, R.
  • Organizer: 2018 International EMECS meeting
  • Int'l Joint Research
• [Presentation] Taxonomic revision of Agaraceae (Laminariales, Phaophyceae) (2017)
  • Author(s): Kawai, H., Hanyuda, T., Gao, X., Terauchi, M., Miyata, M., Lindstrom, S.C., Klochkova, N.G., Miller, K.A.
  • Organizer: British Phycological Society Winter Meeting 2017
  • Place of Presentation: University of Bangor, U.K.
  • Year and Date: 2017-01-11
  • Int'l Joint Research
• [Presentation] Sexual reproductive structures of Lobophora sp. (Dictyotales, Phaeophyceae) from China (2017)
  • Author(s): Sun, Z.M., Tanaka, J., Kawai, H.
  • Organizer: 11th International Phycological Congress (Szczecin, Poland)
  • Int'l Joint Research
• [Presentation] Japan’s nationwide long-term monitoring survey of seaweed communities: ten-year overview and future perspectives (2017)
  • Author(s): Terada, R., Kawai, H., Kurashima, Al, Sakanishi, Y., Simabukuro, H., Dazai, A., Tanaka, J., Murase, N., Motomura, T., Aoki, M., Yamashita, T.
  • Organizer: 11th International Phycological Congress (Szczecin, Poland)
  • Int'l Joint Research
• [Presentation] Taxonomic revision of Eudesme (Ectocarpales s.l., Phaeophyceae). (2017)
  • Author(s): awai, H., Hanyuda, T., Sun, Z.M., Barbara, I., Peters, A.F.
  • Organizer: British Phycological Society Winter Meeting
  • Int'l Joint Research
• [Presentation] Phylogeography of basal taxa of kelps. (2017)
  • Author(s): Kawai, H., Suzuki, M., Hanyuda, T.
  • Organizer: 11th International Phycological Congress (Szczecin, Poland)
  • Int'l Joint Research / Invited
• [Presentation] Early evolution and biogeography of Laminariales (2017)
  • Author(s): Kawai, H.
  • Organizer: 8th Asian Pacific Phycology Forum
  • Int'l Joint Research / Invited
• [Presentation] Inconspicuous flora of the rocky shores: Crustose brown algae (Ralfsiales, Phaeophyceae) from the (sub)tropical Indo-Pacific (2016)
  • Author(s): Poong, S.-W., Lim, P.-E., Phang, S.-M., Sunarpi, H., West, J.A., Lin, S.-M., Nelson, A.W., Miller, K.A, Kawai, H.
  • Organizer: 9th Asia-Pacific Conference on Algal Biotechnology
  • Place of Presentation: Century Park Hotel, Bangkok, Thailand
  • Year and Date: 2016-11-15
  • Int'l Joint Research
• [Presentation] The threat of introduced macroalgal species arriving on Northwestern American shores associated with Japanese tsunami marine debris (JTMD) (2016)
  • Author(s): Kawai, H., Hanyuda, T., Hansen, G.
  • Organizer: 9th Asia-Pacific Conference on Algal Biotechnology
  • Place of Presentation: Century Park Hotel, Bangkok, Thailand
  • Year and Date: 2016-11-15
  • Int'l Joint Research / Invited
• [Presentation] Genetic diversity and biogeography of the macroalgal species associated with the Japanese tsunami marine debris. 2016 (2016)
  • Author(s): Hanyuda, T., Hansen, G., Kawai, H.
  • Organizer: PICES 2016 Annual Meeting
  • Place of Presentation: Omni San Diego Hotel, San Diego, CA, USA.
  • Year and Date: 2016-11-02
  • Int'l Joint Research
• [Presentation] An overview on the crustose brown algal (Phaeophyceae) taxa (2016)
  • Author(s): Poong, S.-W., Lim, P.-E., Phang, S.-M., Sunarpi, H., West, J.A., Lin, S.-M., Kawai, H.
  • Organizer: 30th Annual meeting of the Korean Society of Phycology
  • Place of Presentation: Ramada, Jeju, Korea.
  • Year and Date: 2016-09-27
  • Int'l Joint Research
• [Presentation] The macroalgal culture collection in Kobe University (KU-MACC), and the comprehensive molecular phylogeny of Phaeophyceae based on the culture strains (2016)
  • Author(s): Kawai, H., Terauchi, M., Hanyuda, T.
  • Organizer: The 8th ANRRC International Meeting
  • Place of Presentation: Kyoto University
  • Year and Date: 2016-09-20
  • Int'l Joint Research
• [Presentation] Species diversity and the threat of introduced macroalgal species arriving on Northwestern American shores via Japanese Tunami Marine Debris (JTMD) (2016)
  • Author(s): Kawai, H., Hanyuda, T., Hansen, G.
  • Organizer: 11th EMECS Conference
  • Place of Presentation: Azimut Hotel, St. Petersburg, Russia
  • Year and Date: 2016-08-22
  • Int'l Joint Research
• [Book] Seaweed Phylogeography (2016)
  • Author(s): Kawai, H., Hanyuda, T., Uwai, S. (Ed. Z.Hu and C.Fraser)
  • Total Pages: 395
  • Publisher: Springer