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Commonality and diversity of downstream genes between Brassica self-incompatiblity and plant-pathogen interaction

Research Project

Project/Area Number 16H04854
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Science in genetics and breeding
Research InstitutionTohoku University

Principal Investigator

Watanabe Masao  東北大学, 生命科学研究科, 教授 (90240522)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2018: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2016: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywordsアブラナ科植物 / 自家不和合性 / 耐病性 / 下流因子 / 共通性 / 多様性 / クロストーク / QTL解析 / 情報伝達 / 遺伝学 / 育種学 / バイオインフォマティクス / 細胞内情報伝達
Outline of Final Research Achievements

In order to dissect the self-compatible lines (SC-a and SC-b), linkage molecular markers to SC trait were identified. In the case of SC-a, several SI-related genes were identified. On candidate gene, SC-a-1, RNAi lines were established, and showed partial SC phenotype. In the case of SC-b, this SC gene was tightly linked S gene. Furthermore, I determined the molecular mechanism of dominance relationship within class-II S haplotypes, and novel unilaral incompatibility between Japanese and Turkish population. In addition to the experiments, we also performed the outreach activities to elementary school, high school students.

Academic Significance and Societal Importance of the Research Achievements

アブラナ科植物の自家不和合性における花粉と雌しべとの相互作用は、植物を代表する細胞間コミュニケーションのモデルである。この自己・非自己認識システムの下流がどの様に制御されているのかということは、他の自他識別システムである「耐病性」などとの下流との比較は重要であると考える。また、自家不和合性下流因子の理解は、自家不和合性形質を利用した経済的F1採種において、自家和合性系統の効率的排除にも利用でき、社会的な意義も有している。今回の成果で耐病性との関連性は見出せなかったが、下流因子候補、連鎖している遺伝子を見出せたことは、学術面と社会実装という採種面にも貢献できると判断した。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (46 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (7 results) Journal Article (8 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 8 results,  Acknowledgement Compliant: 3 results) Presentation (24 results) (of which Int'l Joint Research: 12 results,  Invited: 9 results) Book (3 results) Remarks (4 results)

  • [Int'l Joint Research] チューリッヒ大学(スイス)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] 順天大学/忠南大学(韓国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] チューリッヒ大学(スイス)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] 順天大学/忠南大学(韓国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research]

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] チューリッヒ大学(スイス)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] 順天大学/忠南大学(韓国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Abscisic acid-mediated developmental flexibility of stigmatic papillae in response to ambient humidity in <i>Arabidopsis thaliana</i>2018

    • Author(s)
      Takeda, S., Ochiai, K., Egusa, W., Morimoto, H., Sakazono, S., Osaka, M., Nabemoto, M., Kagaya, Y., Suzuki, G., Watanabe, M., and Suwabe, K.
    • Journal Title

      Genes & Genetic Systems

      Volume: 93 Issue: 5 Pages: 209-220

    • DOI

      10.1266/ggs.18-00025

    • NAID

      130007547247

    • ISSN
      1341-7568, 1880-5779
    • Year and Date
      2018-10-01
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Duplicated pollen-pistil recognition loci control intraspecific unilateral incompatibility in Brassica rapa2017

    • Author(s)
      Takada Yoshinobu、Murase Kohji、Shimosato-Asano Hiroko、Sato Takahiro、Nakanishi Honoka、Suwabe Keita、Shimizu Kentaro K.、Lim Yong Pyo、Takayama Seiji、Suzuki Go、Watanabe Masao
    • Journal Title

      Nature Plants

      Volume: 3 Issue: 7 Pages: 17096-17096

    • DOI

      10.1038/nplants.2017.96

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] アブラナ科植物において自他を認識する遺伝子の遺伝子重複と相互の機能喪失により生じた新たな生殖障壁2017

    • Author(s)
      高田美信, 鈴木剛, 渡辺正夫
    • Journal Title

      First Author's

      Volume: - Pages: 074

    • DOI

      10.7875/first.author.2017.074

    • Related Report
      2017 Annual Research Report
    • Open Access
  • [Journal Article] Characterization of two PEBP genes, SrFT and SrMFT, in thermogenic skunk cabbage (Symplocarpus renifolius).2016

    • Author(s)
      Ito-Inaba, Y., Masuko-Suzuki, H., Maekawa, H., Watanabe, M., and Inaba, T.
    • Journal Title

      Sci. Rep.

      Volume: 6 Issue: 1 Pages: 29440-29440

    • DOI

      10.1038/srep29440

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparative analysis of microRNA profiles of rice anthers between cool-sensitive and cool-tolerant cultivars under cool-temperature stress2016

    • Author(s)
      Shunsuke Maeda, Satomi Sakazono, Hiromi Masuko-Suzuki, Midori Taguchi, Kaori Yamamura, Kuniaki Nagano, Takashi Endo, Kenichi Saeki, Masaaki Osaka, Moe Nabemoto, Kana Ito, Toru Kudo, Masaaki Kobayashi, Mizuho Kawagishi, Kotomi Fujita, Hikaru Nanjo, Tomoe Shindo, Kentaro Yano, Go Suzuki, Keita Suwabe, and Masao Watanabe
    • Journal Title

      Genes & Genetic Systems

      Volume: 91 Issue: 2 Pages: 97-109

    • DOI

      10.1266/ggs.15-00056

    • NAID

      130005421208

    • ISSN
      1341-7568, 1880-5779
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Identification of reference genes for quantitative expression analysis using large-scale RNA-seq data of <i>Arabidopsis thaliana</i> and model crop plants2016

    • Author(s)
      Toru Kudo, Yohei Sasaki, Shin Terashima, Noriko Matsuda-Imai, Tomoyuki Takano, Misa Saito, Maasa Kanno, Soichi Ozaki, Keita Suwabe, Go Suzuki, Masao Watanabe, Makoto Matsuoka, Seiji Takayama, Kentaro Yano
    • Journal Title

      Genes & Genetic Systems

      Volume: 91 Issue: 2 Pages: 111-125

    • DOI

      10.1266/ggs.15-00065

    • NAID

      130005421205

    • ISSN
      1341-7568, 1880-5779
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Molecular characterization of genes encoding isoamylase-type debranching enzyme in tuberous root of sweet potato, <i>Ipomoea batatas</i> (L.) Lam.2016

    • Author(s)
      Nabemoto, M., Watanabe, R., Ohsu, M., Sato, K., Otani, M., Nakayachi, O, and Watanabe, M.
    • Journal Title

      Plant Biotechnology

      Volume: 33 Issue: 5 Pages: 351-359

    • DOI

      10.5511/plantbiotechnology.16.0926a

    • NAID

      130005188236

    • ISSN
      1342-4580, 1347-6114
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A complex dominance hierarchy is controlled by polymorphism of small RNAs and their targets2016

    • Author(s)
      Yasuda S., Wada Y., Kakizaki T., Tarutani Y., Miura-Uno E., Murase K., Fujii S., Hioki T., Shimoda T., Takada Y., Shiba H., Takasaki-Yasuda T., Suzuki G., Watanabe M., Takayama S.
    • Journal Title

      Nat. Plants

      Volume: 3 Issue: 1 Pages: 16206-16206

    • DOI

      10.1038/nplants.2016.206

    • NAID

      120005947451

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Developmental flexibility of stigmatic papilla cell in response to abiotic stress in Arabidopsis thaliana.2019

    • Author(s)
      Egusa, W., Takada, M., Takeda, S., Ochiai, K., Sakazono, S., Osaka, M., Kagaya, Y., Suzuki, G., Watanabe, M. and Suwabe, K.
    • Organizer
      Plant and Animal Genome XXVII
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Pollen hydration mechanism on pollination in Arabidopsis thaliana.2019

    • Author(s)
      Ota, T., Mizoguchi, Y., Ando, M., Kagaya, Y., Suzuki, G., Watanabe, M. and Suwabe, K.
    • Organizer
      Plant and Animal Genome XXVII
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] アブラナ科植物における自家不和合性決定花粉因子の複対立遺伝子間優劣性制御機構.2018

    • Author(s)
      安田晋輔, 和田七夕子, 柿崎智博, 樽谷芳明, 宇野栄子, 村瀬浩司, 藤井壮太, 日置智也, 下田大貴, 高田美信, 柴博史, 安田剛志, 鈴木剛, 渡辺正夫, 高山誠司
    • Organizer
      日本育種学会 第134回講演会ワークショップ
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 自家不和合性遺伝子の重複と相互機能欠失によるアブラナ科植物の一側性不和合性機構.2018

    • Author(s)
      高田美信, 鈴木剛, 清水健太郎, 高山誠司, 渡辺正夫
    • Organizer
      日本育種学会 第134回講演会ワークショップ
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Toward to collaborative research with Brassica crops between Japan and Korea.2018

    • Author(s)
      Watanabe, M.
    • Organizer
      Improvement of important agricultural traits based on genomic information of horticultural crops
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Morphological aspect and molecular mechanism of pollen hydration on pollination in Arabidopsis thaliana.2018

    • Author(s)
      Ota, T., Mizoguchi, Y., Ando, M., Kagaya, Y., Suzuki, G., Watanabe, M. and Suwabe, K.
    • Organizer
      International Plant Molecular Biology 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Novel unilateral incompatibility in Brassica rapa is regulated by duplicated self-incompatibility genes, PUI1 and SUI1.2018

    • Author(s)
      Takada, Y., Murase, K., Shimosato-Adano, H., Sato, T., Nakanishi, H., Suwabe, K., Shimizu, K. K., Lim, Y. P., Takayama, S., Suzuki, G., and Watanabe, M.
    • Organizer
      International Plant & Animal Genome XXVI,
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Self-incompatibility in cruciferous plants -Molecular mechanisms and recent topics-2018

    • Author(s)
      Watanabe, M.
    • Organizer
      International Symposium of Plant Science in UC San Diego
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ABA-mediated flexibility of female stigmatic papillae development in Arabidopsis thaliana.2017

    • Author(s)
      Egusa W, Takeda S, Ochiai K, Morimoto H, Sakazono S, Osaka M, Nabemoto M, Kagaya Y, Suzuki G, Watanabe M, Suwabe K
    • Organizer
      International Plant & Animal Genome XXV
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2017-01-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Pollen-cysteine rich peptide CR3 regulates pollen germination in Arabidopsis thaliana.2017

    • Author(s)
      Morimoto H, Egusa W, Arakawa C, Masuko-Suzuki H, Suzuki G, Watanabe M, Suwabe K
    • Organizer
      International Plant & Animal Genome XXV
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2017-01-14
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification and characterization of the novel pollen-stigma recognition factors for unilateral incompatibility in Brassica rapa.2017

    • Author(s)
      Takada, Y., Murase, K., Shimosato-Adano, H., Sato, T., Nakanishi, H., Suwabe, K., Shimizu, K. K., Lim, Y. P., Takayama, S., Suzuki, G., and Watanabe, M.
    • Organizer
      Taiwan-Japan Plant Biology 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular mechanisms of self-incompatibility in Brassica.2017

    • Author(s)
      Watanabe, M.
    • Organizer
      Plant Biology at Tohoku University
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Brassica rapaの種内一側性不和合性を制御するSUI1-PUI1遺伝子2017

    • Author(s)
      高田美信, 鈴木剛, 村瀬浩司, 浅野(下里)裕子, 佐藤陽洋, 中西ほのか, 諏訪部圭太, Lim Yong Pyo, 清水健太郎, 高山誠司, 渡辺正夫
    • Organizer
      日本植物細胞分子生物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 自己花粉を認識するリガンド・レセプター遺伝子セットの重複が非自己花粉の拒絶を引き起こす2017

    • Author(s)
      高田美信, 鈴木剛, 中西ほのか, 村瀬浩司, 浅野(下里)裕子, 佐藤陽洋, 諏訪部圭太, Lim Yong Pyo, 清水健太郎, 高山誠司, 渡辺正夫
    • Organizer
      日本遺伝学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Brassica rapaのA04染色体には新規生殖障壁遺伝子が存在する2017

    • Author(s)
      高田美信, 中西ほのか, 村瀬浩司, 浅野(下里)裕子, 佐藤陽洋, Yong Pyo Lim, 清水健太郎, 高山誠司, 諏訪部圭太, 鈴木剛, 渡辺正夫
    • Organizer
      日本染色体学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 出前講義から垣間見た日本におけるSTEM教育の現状2017

    • Author(s)
      渡辺正夫, 別府和則, 高橋典子, 寺岸俊哉, 安藤晃
    • Organizer
      第41回日本科学教育学会年会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 東北大学 飛翔型「科学者の卵養成講座」における受講生の能力伸長2017

    • Author(s)
      下山せいら, 渡辺正夫, 伊藤幸博, 久利美和, 安藤晃
    • Organizer
      第41回日本科学教育学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Brassica rapaの種内一側性不和合性を支配する花粉・柱頭認識因子の決定.2017

    • Author(s)
      高田美信, 村瀬浩二, 浅野(下里)裕子, 佐藤陽洋, 中西ほのか, 諏訪部圭太, Lim Yong Pyo, 清水健太郎, 高山誠司, 鈴木剛, 渡辺正夫
    • Organizer
      日本育種学会第132回講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ウメ不稔性花粉の蛍光とミツバチ訪花行動への影響の考察2017

    • Author(s)
      森信之介, 新間秀一, 増子(鈴木)潤美, 渡辺正夫, 中西テツ, 月岡淳子, 福井宏至, 後藤勝実, 平井伸博
    • Organizer
      日本花粉学会第58回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ウメ花粉の発達異常と蛍光およびそのミツバチ訪花行動への影響2017

    • Author(s)
      森信之介, 新間秀一, 増子(鈴木)潤美, 渡辺正夫, 中西テツ, 月岡淳子, 福井宏至, 後藤勝実, 平井伸博
    • Organizer
      植物科学調節学会第52回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 東北大学 飛翔型「科学者の卵養成講座」における受講生のアイスブレイクとなる実技課題の効果2017

    • Author(s)
      下山せいら, 安藤晃, 渡辺正夫, 伊藤幸博, 久利美和, 中村肇
    • Organizer
      平成29年度第3回日本科学教育学会研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 歴史史料・図像等の文化資源よりみたアブラナ科植物の形質等の変異に関する萌芽的研究. 人文情報学研究の最前線20172017

    • Author(s)
      武田和哉, 渡辺正夫, 吉川真司
    • Organizer
      The Leading Edges of Humane Informatics 2017
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] ABA-mediated stress response mechanism modulates stigmatic papillae development in Arabidopsis thaliana.2017

    • Author(s)
      Egusa, W., Takeda, S., Ochiai, K., Morimoto, H., Kagaya, Y., Suzuki, G., Watanabe, M., and Suwabe, K.
    • Organizer
      Global Conference on Plant Science and Molecular Biology
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] CR3, a pollen cysteine-rich peptide, induces pollen germination.2016

    • Author(s)
      Morimoto H, Egusa W, Arakawa C, Masuko-Suzuki H, Suzuki G, Watanabe M, Suwabe M
    • Organizer
      The International Conference on Arabidopsis Research
    • Place of Presentation
      Gyeongju, Korea
    • Year and Date
      2016-06-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Book] 遺伝単 "植物における生殖の不思議と自家不和合性"2017

    • Author(s)
      渡辺正夫
    • Total Pages
      371
    • Publisher
      エヌ・ティー・エス出版
    • Related Report
      2017 Annual Research Report
  • [Book] コマツナ栽培品種とトルコ由来系統間の交雑に生じた一側性不和合性の認識機構2017

    • Author(s)
      高田美信, 鈴木剛, 渡辺正夫
    • Total Pages
      100
    • Publisher
      アグリバイオ
    • Related Report
      2017 Annual Research Report
  • [Book] "植物の性", In "植物学の百科事典"2016

    • Author(s)
      渡辺正夫
    • Total Pages
      802
    • Publisher
      丸善出版
    • Related Report
      2016 Annual Research Report
  • [Remarks] 東北大学大学院生命科学研究科・植物分子育種分野・渡辺研究室・基盤研究(B)

    • URL

      http://www.ige.tohoku.ac.jp/prg/watanabe/project-kiban-b-si/

    • Related Report
      2018 Annual Research Report
  • [Remarks] 基盤研究(B) アブラナ科植物の自家不和合性と耐病性を制御する下流因子の共通性とクロストーク

    • URL

      http://www.ige.tohoku.ac.jp/prg/watanabe/project-kiban-b-si/

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Remarks] 自家不和合性遺伝子の「遺伝子重複」が、離れた地域間での生殖を妨げる仕組みを世界初で証明

    • URL

      http://www.ige.tohoku.ac.jp/prg/watanabe/news2/2017/07/28171713.php

    • Related Report
      2017 Annual Research Report
  • [Remarks] 対立遺伝子の組合せで優劣性が変化する分子メカニズムは低分子RNAで制御、世界初で証明

    • URL

      http://www.ige.tohoku.ac.jp/prg/watanabe/news2/2016/12/24175651.php

    • Related Report
      2016 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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