Analysis of molecular and genetic mechanism of super water-repellency in rice

• Project/Area Number: 16H04857
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: The University of Tokyo
• Principal Investigator: Itoh Jun-ichi 東京大学, 大学院農学生命科学研究科(農学部), 准教授 (30345186)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000); Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: 撥水性 / イネ / 遺伝学 / 変異体 / 葉 / 遺伝子 / ワックス / 植物 / 発生・分化 / 表皮

Outline of Final Research Achievements

Rice leaf shows extremely high water-repellency (super water-repellency) as much as that of lotus leaf. In this analysis, expression mechanism for the super water-repellency of rice leaves were elucidated in various points of view. First, it revealed that the adaxial side of leaf blade in rice showed the highest water-repellency and the water-repellency was changed depending on different environmental conditions. In addition, it was shown that several wax biosynthetic genes were important for the water-repellency by using wetting leaf mutants. Furthermore, it was suggested that many rice cultivars show high water-repellency.

Academic Significance and Societal Importance of the Research Achievements

超撥水性は植物の耐病性や光合成効率の上昇に寄与すると考えられる。本研究で得られた成果によって植物表面の撥水性を制御する基盤的知識を得ることができた。これを利用して、作物における病原体による初期感染の予防や、汚れの洗浄効果による病原体の感染リスクの低下、光合成効率の上昇が期待できる。また、イネにおけるワックス合成遺伝子の解明により、イネワックスの改変や増産の可能性も期待される。

Research Products

• [Journal Article] BLADE-ON-PETIOLE genes temporally and developmentally regulate the sheath to blade ratio of rice leaves (2019)
  • Author(s): Taiyo Toriba, Hiroki Tokunaga, Toshihide Shiga, Fanyu Nie, Satoshi Naramoto, Eriko Honda, Keisuke Tanaka, Teruaki Taji, Jun-Ichi Itoh, Junko Kyozuka
  • Journal Title: Nature communications Volume: 10 Issue: 1 Pages: 619-619
  • DOI: 10.1038/s41467-019-08479-5
  • Peer Reviewed / Open Access
• [Journal Article] Genetic mechanisms involved in the formation of root system architecture (2018)
  • Author(s): Yuka Kitomi, Jun-Ichi Itoh, Yusaku Uga
  • Journal Title: Rice Genomics, Genetics and Breeding Volume: - Pages: 241-274
  • DOI: 10.1007/978-981-10-7461-5_14
  • ISBN: 9789811074608, 9789811074615
  • Peer Reviewed
• [Journal Article] Conserved functional control, but distinct regulation of cell proliferation in rice and Arabidopsis leaves revealed by comparative analysis of GRF-INTERACTING FACTOR 1 orthologs. (2018)
  • Author(s): Shimano, S., Hibara, K., Furuya, T., Arimura, S., Tsukaya,H. and Itoh, J.I.
  • Journal Title: Development Volume: 175 Issue: 7 Pages: 159624-159624
  • DOI: 10.1242/dev.159624
  • Peer Reviewed / Open Access
• [Journal Article] Rice leaf hydrophobicity and gas films are conferred by a wax synthesis gene (LGF1) and contribute to flood tolerance. (2018)
  • Author(s): Kurokawa, Y., Nagai, K., Huan, P. D., Shimazaki, K., Qu, H., Mori, Y., Toda, Y., Kuroha, T., Hayashi, N., Aiga, S., Itoh, J.-i., Yoshimura, A., Sasaki-Sekimoto, Y., Ohta, H., Shimojima, M., Malik, A. I., Pedersen, O., Colmer, T. D. and Ashikari, M.
  • Journal Title: New Phytologist Volume: － Issue: 4 Pages: 1558-1569
  • DOI: 10.1111/nph.15070
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genetic Mechanisms Involved in the Formation of Root System Architecture. (2018)
  • Author(s): Kitomi, Y., Itoh, JI., Uga, Y.
  • Journal Title: In: Sasaki T., Ashikari M. (eds) Rice Genomics, Genetics and Breeding. Volume: － Pages: 241-274
• [Journal Article] LEAF LATERAL SYMMETRY1, a member of the WUSCHEL-RELATED HOMEOBOX3 gene family, regulates lateral organ development differentially from other paralogs, NARROW LEAF2 and NARROW LEAF3 in rice. (2017)
  • Author(s): Eriko, H., Yew, C.L., Yoshikawa, T., Sato, Y., Hibara, K. and Itoh, J.I.
  • Journal Title: Plant and Cell Physiology Volume: 59 Issue: 2 Pages: 376-391
  • DOI: 10.1093/pcp/pcx196
  • Peer Reviewed / Open Access
• [Journal Article] Barley <i>NARROW LEAFED DWARF1</i> encoding a WUSCHEL-RELATED HOMEOBOX 3 (WOX3) regulates the marginal development of lateral organs (2016)
  • Author(s): Yoshikawa, T., Tanaka, S., Masumoto, Y., Nobori, N., Ishii, H., Hibara, K., Itoh, J., Tanisaka, T., and Taketa, S.
  • Journal Title: Breeding Science Volume: 66 Issue: 3 Pages: 416-424
  • DOI: 10.1270/jsbbs.16019
  • NAID: 130005160889
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed / Open Access
• [Journal Article] Jasmonate regulates juvenile-adult phase transition in rice. (2016)
  • Author(s): Hibara, K., Isono, M., Mimura, M., Sentoku, N., Kojima, M., Sakakibara, H., Kitomi, Y., Yoshikawa, T., Itoh, J., and Nagato, Y.
  • Journal Title: Development Volume: 143 Pages: 3407-3416
  • DOI: 10.1242/dev.138602
  • Peer Reviewed / Open Access
• [Presentation] イネGE遺伝子のシュート再生過程における機能解析 (2019)
  • Author(s): 八田大成・水野泉・桧原健一郎・伊藤純一
  • Organizer: 日本育種学会135回講演会
• [Presentation] 遺伝子発現解析によるイネの葉の形態形成に関わる遺伝子の探索 (2018)
  • Author(s): 味谷雅之・吉川貴徳・佐藤豊・長村吉晃・伊藤純一
  • Organizer: 日本育種学会134回講演会
• [Presentation] 植物の成長制御に関与するCYP78のイネのカルス組織に対する効果 (2018)
  • Author(s): 八田大成・水野泉・桧原健一郎・伊藤純一
  • Organizer: 日本育種学会134回講演会
• [Presentation] イネの葉における撥水性の制御機構と多様性の解析 (2017)
  • Author(s): 平岩飛鳥・相賀彩織・佐藤豊・伊藤純一
  • Organizer: 日本育種学会 132回講演会
• [Presentation] embryoless変異体を用いたイネの胚・胚乳サイズ比率制御機構の解析 (2017)
  • Author(s): 金川真美・松本和也・石川亮・伊藤純一・桧原健一郎
  • Organizer: 日本育種学会 132回講演会
• [Presentation] イネの胚性器官に異常を示す変異体の解析 (2016)
  • Author(s): 伊久力也，伊藤純一，桧原健一郎
  • Organizer: 日本育種学会
  • Place of Presentation: 鳥取大学（鳥取県 鳥取市）
• [Presentation] イネにおけるLOG遺伝子ホモログの発現解析 (2016)
  • Author(s): 横田 絢，伊藤純一，桧原健一郎
  • Organizer: 日本育種学会
  • Place of Presentation: 鳥取大学（鳥取県 鳥取市）