A study on sweetness and flowering time as a basis for biorefinery optimization breeding of sorghum

• Project/Area Number: 16H04859
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: Nagoya University
• Principal Investigator: SAUZKA TAKASHI 名古屋大学, 生物機能開発利用研究センター, 准教授 (70362291)
• Research Collaborator: WU Jianzhong 農業・食品産業技術総合研究機構
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000); Fiscal Year 2018: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2017: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2016: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
• Keywords: ソルガム / バイオリファイナリー / PRR37 / 高糖性 / 開花期 / 育種学

Outline of Final Research Achievements

In the biorefinery industry such as bioethanol and bioplastics production for the "non-petroleum society", the basic raw material is sugar. In this study, we focused on the high sugar content of sweet sorghum juice and analyzed its responsible locus qBRX-6. As a result, (1) qBRX-6 is considered to be a complex locus of multiple responsible genes, and also influenced by environmental factors. (2) As a result of narrowing down one of the responsible genomic regions, five genes were assigned. (3) As a result of examining also the possibility of SbPRR37 as another candidate gene, the sweet sorghum allele is partial loss-of-function, which may have an influence on the translocation of sugar in plant.

Academic Significance and Societal Importance of the Research Achievements

現代・未来社会において、エネルギー創出に貢献するバイオエタノール生産や、バイオプラスチック生産などのバイオリファイナリー産業の基本原料は糖である。しかし、これまでサトウキビなどの糖原料作物において、糖の産生の原因遺伝子は明らかにされていなかった。本研究成果は、スイートソルガムを糖原料作物のモデル作物と位置付け、遺伝学的及び分子遺伝学的観点から、どのような遺伝子座及び遺伝子が糖の産生に関与しているのかについて解析を進め、学術的理解を深めた。さらに低炭素社会など、持続可能な開発目標（SDGs）の達成に向けた社会の基盤作りへ向けて、農学や育種学の貢献が重要であるという社会的意義を示した。

Research Products

• [Journal Article] Repeated ethanol fermentation from membrane-concentrated sweet sorghum juice using the flocculating yeast Saccharomyces cerevisiae F118 strain. (2018)
  • Author(s): Hans Wijaya, Kengo Sasaki, Prihardi Kahar, Yopi, Hideo Kawaguchi, Takashi Sazuka, Chiaki Ogino, Bambang Prasetya, Akihiko Kondo
  • Journal Title: Bioresour Technol. Volume: 265 Pages: 542-547
  • DOI: 10.1016/j.biortech.2018.07.039
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Effective usage of sorghum bagasse: Optimization of organosolv pretreatment using 25% 1-butanol and subsequent nanofiltration membrane separation (2018)
  • Author(s): Teramura Hiroshi、Sasaki Kengo、Oshima Tomoko、Kawaguchi Hideo、Ogino Chiaki、Sazuka Takashi、Kondo Akihiko
  • Journal Title: Bioresource Technology Volume: 252 Pages: 157-164
  • DOI: 10.1016/j.biortech.2017.12.100
  • Peer Reviewed
• [Journal Article] Heap: a highly sensitive and accurate SNP detection tool for low-coverage high-throughput sequencing data (2017)
  • Author(s): Kobayashi Masaaki、Ohyanagi Hajime、Takanashi Hideki、Asano Satomi、Kudo Toru、Kajiya-Kanegae Hiromi、Nagano Atsushi J.、Tainaka Hitoshi、Tokunaga Tsuyoshi、Sazuka Takashi、Iwata Hiroyoshi、Tsutsumi Nobuhiro、Yano Kentaro
  • Journal Title: DNA Research Volume: 24 Issue: 4 Pages: 397-405
  • DOI: 10.1093/dnares/dsx012
  • Peer Reviewed / Open Access
• [Journal Article] Sucrose Purification and Repeated Ethanol Production from Sugars Remaining in Sweet Sorghum Juice Subjected to a Membrane Separation Process (2017)
  • Author(s): Kengo Sasaki, Yota Tsuge, Hideo Kawaguchi, Masahiro Yasukawa, Daisuke Sasaki, Takashi Sazuka, Eiji Kamio, Chiaki Ogino, Hideto Matsuyama and Akihiko Kondo
  • Journal Title: App Micbio Biotech Volume: 101 Issue: 15 Pages: 6007-6014
  • DOI: 10.1007/s00253-017-8316-3
  • Peer Reviewed
• [Journal Article] Screening of rice mutants with improved saccharification efficiency results in the identification of CONSTITUTIVE PHOTOMORPHOGENIC 1 and GOLD HULL AND INTERNODE 1 (2017)
  • Author(s): Ko Hirano, Reiko Masuda, Wakana Takase, Yoichi Morinaka, Mayuko Kawamura, Yoshinobu Takeuchi, Hiroki Takagi, Hiroki Yaegashi, Satoshi Natsume, Ryouhei Terauchi, Toshihisa Kotake, Yasuyuki Matsushita, Takashi Sazuka.
  • Journal Title: Planta Volume: 未定 Issue: 1 Pages: 61-74
  • DOI: 10.1007/s00425-017-2685-9
  • NAID: 120006349150
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Differences in Glucose Yield of Residues from among Varieties of Rice, Wheat, and Sorghum after Dilute Acid Pretreatment (2017)
  • Author(s): Hiroshi Teramura, Kengo Sasaki, Hideo Kawaguchi, Fumio Matsuda, Jun Kikuchi, Tomokazu Shirai, Takashi Sazuka, Masanori Yamasaki, Shigeo Takumi, Chiaki Ogino and Akihiko Kondo
  • Journal Title: Biosci Biotech Biochem Volume: 81 Issue: 8 Pages: 1650-1656
  • DOI: 10.1080/09168451.2017.1336922
  • Peer Reviewed
• [Journal Article] Sorghum DW1 positively regulates brassinosteroid signaling by inhibiting the nuclear localization of BRASSINOSTEROID INSENSITIVE 2. (2017)
  • Author(s): Hirano K, Kawamura M, Araki-Nakamura S, Fujimoto H, Ohmae-Shinohara K, Yamaguchi M, Fujii A, Sasaki H, Kasuga S, Sazuka T.
  • Journal Title: Sci Rep. Volume: 1 Issue: 1 Pages: 126-126
  • DOI: 10.1038/s41598-017-00096-w
  • Peer Reviewed / Open Access
• [Journal Article] Sorghum Dw1, an agronomically important gene for lodging resistance, encodes a novel protein involved in cell proliferation. (2016)
  • Author(s): Yamaguchi M, Fujimoto H, Hirano K, Araki-Nakamura S, Ohmae-Shinohara K, Fujii A, Tsunashima M, Song XJ, Ito Y, Nagae R, Wu J, Mizuno H, Yonemaru J, Matsumoto T, Kitano H, Matsuoka M, Kasuga S, Sazuka T.
  • Journal Title: Sci Rep. Volume: 6 Issue: 1 Pages: 28366-28366
  • DOI: 10.1038/srep28366
  • Peer Reviewed / Open Access
• [Presentation] 作物ゲノムのデザイニング ―社会と向き合う植物科学― (2018)
  • Author(s): 佐塚隆志
  • Organizer: 植物科学シンポジウム
  • Invited
• [Presentation] ソルガムのゲノムデザイン (2018)
  • Author(s): 佐塚隆志
  • Organizer: 第9回ソルガムワークショップ
• [Presentation] An agronomically important gene, Dw1, encodes a novel component of brassinosteroid signaling, and controls the cell proliferation in internodes (2018)
  • Author(s): Takashi Sazuka, Ko Hirano, Satoko Araki-Nakamura, Kozue Ohmae-Shinohara, Mayuko Kawamura, Haruka Fujimoto, Miki Yamaguchi, Akihiro Fujii, Shigemitsu Kasuga
  • Organizer: Sorghum in the 21st Century
  • Int'l Joint Research
• [Presentation] スイートソルガムのバイオリファイナリー利用を目指した糖収量性関連形質の解析 (2018)
  • Author(s): 丹羽佑介、中村(荒木)聡子、南山将輝、篠原(大前)梢、川口秀夫、三浦孝太郎、春日重光、佐塚隆志
  • Organizer: 日本育種学会第134回講演会
• [Presentation] ソルガム高バイオマスF1品種「天高」の稈径とバイオマス関連形質に関するQTL解析 (2018)
  • Author(s): 和氣達朗、中村（荒木）聡子、篠原（大前）梢、三浦孝太郎、春日重光、佐塚隆志
  • Organizer: 日本育種学会第135回講演会
• [Presentation] 高バイオマス・高糖収量性を併せ持つバイオリファイナリーに最適化したソルガム新品種「炎龍」の育成 (2017)
  • Author(s): 佐塚隆志、藤井昭裕、中村（荒木）聡子、和田多門、山口未来、岡村進之介、篠原（大前）梢、伊藤祐介、松岡信、北野英己、春日重光
  • Organizer: 日本育種学会第130回講演会
  • Place of Presentation: 名古屋
  • Year and Date: 2017-03-30
• [Presentation] ソルガム搾汁液の糖収量に関する育種遺伝学的解析 (2017)
  • Author(s): 藤井昭裕、和田多門、中村（荒木）聡子、倉見慶二郎、篠原（大前）梢、伊藤祐介、北野英己、松岡信、春日重光、佐塚隆志
  • Organizer: 日本育種学会第130回講演会
  • Place of Presentation: 名古屋
  • Year and Date: 2017-03-30
• [Presentation] Dw1, an Important Gene for Lodging Resistance and Mechanical Harvesting of Sorghum, Encodes a Novel Protein Involved in Cell Proliferation. (2017)
  • Author(s): Sazuka, T
  • Organizer: Plant and Animal Genome XXV
  • Place of Presentation: San Diego
  • Year and Date: 2017-01-15
  • Int'l Joint Research / Invited
• [Presentation] 組換え大腸菌によるフェニル乳酸における多様な草本系バイオマス糖化液の発酵阻害とその成分の比較 (2017)
  • Author(s): 川口秀夫、吉原久美子、佐塚隆志、高谷直樹、蓮沼誠久、寺村浩、荻野千秋、近藤明彦
  • Organizer: 日本農芸化学会関西支部第501回講演会
• [Presentation] 花粉親品種における開花期改良の試み (2017)
  • Author(s): 佐々木碩哲、和田多門、中村聡子、大前梢、岡村進之介、 藤井昭裕、春日重光、佐塚隆志
  • Organizer: 第8回ソルガムワークショップ
• [Presentation] スイートソルガムの有する新規高糖性QTLの同定を目指した遺伝学的解析 (2017)
  • Author(s): 丹羽佑介、岡村進之介、中村（荒木）聡子、河江琴奈、篠原（大前）梢、三浦 孝太郎、春日重光、佐塚 隆志
  • Organizer: 第25回育種学会中部地区談話会
• [Presentation] 輪郭形状のゲノミック予測のための定量化手法の比較 (2017)
  • Author(s): 坂本莉沙、鐘ケ江弘美、野下浩司、石森元幸、小林正明、藤本優、高梨秀樹、永野惇、佐塚隆志、矢野健太郎、徳永毅、堤伸浩、岩田洋佳
  • Organizer: 日本育種学会第133回講演会
• [Book] Int Rev Cell Mol Biol., Molecular breeding of Sorghum bicolor: A Novel Energy Crop. (2016)
  • Author(s): Ordonio R, Ito Y, Morinaka Y, Sazuka T, and Matsuoka M.
  • Total Pages: 37
  • Publisher: Elsevier Inc.