| Budget Amount *help |
¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Outline of Final Research Achievements |
In this project, we examined heat tolerance mechanisms in rice spikelet fertility by using real-time single pollen metabolomics and gene expression analysis. Using picolitre pressure-probe-electrospray-ionization mass spectrometry (picoPPESI-MS) to directly determine the metabolites and microarray analysis in the single mature pollen grains of two cultivars that exhibited different heat tolerance. The metabolome analysis detected varietal differences in phosphatidylinositol (PI) in mature pollen, together with other 106 metabolites including cysteine. Greater PI content was detected in heat-tolerant cultivar pollen regardless of the treatment, but not for heat-susceptible cultivar pollen. Microarray analysis has also suggested the importance of expression of lipolytic enzyme-encoded genes at high temperature. Therefore, we conclude that the observed active synthesis of PI prior to germination may be an important factor for maintaining spikelet fertility under heat conditions.
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