New Frontier in fruit physiology - Ethylene independent fruit ripening by low-temperature exposure

• Project/Area Number: 16H04873
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Horticultural science
• Research Institution: Okayama University
• Principal Investigator: KUBO Yasutaka 岡山大学, 環境生命科学研究科, 教授 (80167387)
• Co-Investigator(Kenkyū-buntansha): 矢野 健太郎 明治大学, 農学部, 専任教授 (00446543) ; 中野 龍平 京都大学, 農学研究科, 准教授 (70294444)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000); Fiscal Year 2019: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
• Keywords: 果実成熟 / 低温 / エチレン / RNAseq / クライマクテリック型果実 / 転写因子 / 遺伝子発現 / 低温誘導

Outline of Final Research Achievements

Low temperature condition has been believed to delay fruit ripening and extend storage life. Contrary to the common sense, we demonstrated that fruit ripening can be accelerated by low temperature exposure in European pears, citrus and kiwifruit, in ethylene independent manner in the two latter fruit. The low temperature modulated ripening could be a purposeful phenomenon for these fruits to be eaten and seed scattered by insects and animals.

Academic Significance and Societal Importance of the Research Achievements

本研究で明らかにした低温誘導性果実成熟は、クライマクテリック型果実でのエチレン生合成と信号伝達に偏って研究されてきた果実成熟生理研究に新展開をもたらし、ノンクライマクテリック型果実での非エチレン成熟も含めた統一的理解に貢献する。低温感受性は果実成熟の早晩生に密接に関与しており、その機構解明と本研究で示した低温応答性鍵転写因子候補の特定は早生・晩生品種の効率的育種につながる。また、低温成熟は徐々に進むために果実の適食期間を大幅に伸ばす品質保持技術開発に直結する。

Research Products

• [Int'l Joint Research] ジョモケニアッタ農工大学(ケニア)
• [Int'l Joint Research] ジョモケニアッタ農工大学/メルー大学(ケニア)
• [Journal Article] ‘Passe Crassane’ pear fruit (Pyrus communis L.) ripening: Revisiting the role of low temperature via integrated physiological and transcriptome analysis. (2019)
  • Author(s): Mitalo O.W., Tosa Y., Tokiwa S., Kondo Y., Azimi A., Hojo Y., Matsuura T., Mori I.C., Nakano R., Akagi T., Ushijima K., Kubo Y.
  • Journal Title: Post harvest Biology and Technology Volume: 158 Pages: 1-14
  • DOI: 10.1016/j.postharvbio.2019.110949
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Low temperature storage stimulates fruit softening and sugar accumulation without ethylene and aroma volatile production in kiwifruit. (2019)
  • Author(s): Mitalo O.W., Tokiwa S., Kondo Y., Otsuki T., Galis I., Suezawa K., Kataoka I., Doan A.T., Nakano R., Ushijima K., Kubo
  • Journal Title: Frontiers in Plant Science Volume: 10 Pages: 1-15
  • DOI: 10.3389/fpls.2019.00888
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comparative analysis of fruit ripening and associated genes in two kiwifruit cultivars (‘Sanuki Gold’ and ‘Hayward’) at various storage temperatures. (2019)
  • Author(s): Mitalo O. W., Asiche W. O., Kasahara Y., Tosa Y., Tokiwa S., Ushijima K., Nakano R., Kubo Y.
  • Journal Title: Postharvest Biology and Technology Volume: 147 Pages: 20-28
  • DOI: 10.1016/j.postharvbio.2018.08.017
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Determination of optimum temperature for long-term storage and analysis of ripening-related genes in ‘Rainbow Red’ kiwifruit (2018)
  • Author(s): Mitalo O.W., Tokiwa S., Kasahara Y., Tosa Y., Kondo Y., Asiche W.O., Kataoka I., Suezawa K., Ushijima K., Nakano R., Kubo Y.
  • Journal Title: Acta Horticulturae Volume: 1218 Issue: 1218 Pages: 517-524
  • DOI: 10.17660/actahortic.2018.1218.71
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Characterization of Ripening-related Genes Involved in Ethylene-independent Low Temperature-modulated Ripening in ‘Rainbow Red’ Kiwifruit during Storage and On-vine (2018)
  • Author(s): Mitalo, W.O.,W. O. Asiche., Y. Kasahara, Y.i Tosa, W. O. Owino, E. G. Mworia, K. Ushijima, R.Nakano and Y. Kubo
  • Journal Title: The Horticulture Journal Volume: 87 Issue: 3 Pages: 421-429
  • DOI: 10.2503/hortj.OKD-035
  • NAID: 130007420786
  • ISSN: 2189-0102, 2189-0110
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comparative transcriptome analysis reveals distinct ethylene?independent regulation of ripening in response to low temperature in kiwifruit (2018)
  • Author(s): Asiche William O.、Mitalo Oscar W.、Kasahara Yuka、Tosa Yasuaki、Mworia Eric G.、Owino Willis O.、Ushijima Koichiro、Nakano Ryohei、Yano Kentaro、Kubo Yasutaka
  • Journal Title: BMC Plant Biology Volume: 18 Issue: 1 Pages: 47-47
  • DOI: 10.1186/s12870-018-1264-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effect of Storage Temperature on Fruit Ripening in Three Kiwifruit Cultivars (2017)
  • Author(s): Asiche W. O., Mitalo O. W., Kasahara Y., Tosa Y., Mworia E. G., Ushijima K., Nakano R., Kubo Y.
  • Journal Title: The Horticulture Journal Volume: 86 Issue: 3 Pages: 403-410
  • DOI: 10.2503/hortj.OKD-028
  • NAID: 130005835934
  • ISSN: 2189-0102, 2189-0110
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Examining ethylene and low temperature effects on peel color change in lemons. (2019)
  • Author(s): Mitalo O.W., Oji N., Kondo Y., Otsuki T., Azimi A., Kazimi A.H., Akagi T., Ushijima K., Kubo Y.
  • Organizer: The 16th Japan Solanaceae Consortium.
  • Int'l Joint Research
• [Presentation] Physiological and transcriptomic analysis of temperature-dependent color change in lemon fruit. (2019)
  • Author(s): Mitalo O.W., Oji N., Kondo Y., Otsuki T., Okada R., Kawai T., Akagi T., Ushijima K., Kubo Y.
  • Organizer: 園芸学会2019年秋季大会
• [Presentation] セイヨウナシとチュウゴクナシにおける成熟特性の比較 (2019)
  • Author(s): 近藤憂紀・土佐康彰・Oscar MITALO・常盤淑玲・Abudle AZIMI・大槻巧・久保康隆他３名
  • Organizer: 園芸学会2019年秋季大会
• [Presentation] 1-MCP処理と貯蔵温度がカキ’富有’の果実軟化に及ぼす影響 (2019)
  • Author(s): 大槻巧・Oscar MITALO・常盤淑玲・近藤憂紀・Abudle AZIMI・久保康隆他４名
  • Organizer: 園芸学会2019年秋季大会
• [Presentation] Probing the role of ethylene and low temperature in the modulation of flavedo colour change in Satsuma mandarins (Citrus unshiu Marc) fruit. (2018)
  • Author(s): Mitalo O. W., Tosa Y., Kasahara Y., Tokiwa S., Kondo Y., Azimi A., Ushijima K., Nakano R., Kubo Y.
  • Organizer: The XI International Symposium on the Plant Hormone Ethylene
  • Int'l Joint Research
• [Presentation] Comparison of ethylene induced ripening and low temperature modulated ripening in kiwifruit. (2018)
  • Author(s): Kubo Y., Mitalo O. W., Tosa Y., Kasahara Y., Tokiwa S., Kondo Y., Azimi A., Galis I., Ushijima K., Nakano R.
  • Organizer: The XI International Symposium on the Plant Hormone Ethylene
  • Int'l Joint Research
• [Presentation] Transcriptome analysis provides new insights into the regulation of chilling-induced ripening in ‘Passe Crassane’ pear. (2018)
  • Author(s): Kondo Y., Mitalo O. W., Tosa Y., Kasahara Y., Tokiwa S., Azimi A., Ushijima K., Nakano R., Kubo
  • Organizer: The XI International Symposium on the Plant Hormone Ethylene
  • Int'l Joint Research
• [Presentation] Transcriptional analysis of flavedo color changes in detached and on-tree Satsuma Mandarin (Citrus unshiu Marc.) fruit. (2018)
  • Author(s): Mitalo O. W., Tosa Y., Kasahara Y., Tokiwa S., Kondo Y., Oji N., Otsuki T., Doan A. T., Ushijima K., Nakano R., Kubo Y.
  • Organizer: 平成１８年度園芸学会秋季大会
• [Presentation] 低温誘導による果実の成熟誘導とその利用 (2018)
  • Author(s): 久保康隆
  • Organizer: 平成１８年度園芸学会秋季大会
  • Invited
• [Presentation] ‘香粋’キウイフルーツにおける芳香成分と一次代謝産物のGC/MSによる解析 (2018)
  • Author(s): 常盤淑玲、笠原有加、土佐康彰、Oscar Witere Mitalo、近藤憂紀、大槻巧、大地菜摘、Azimi Azimullah、Doan Anh Thu、Ivan Galis、牛島幸一郎、中野龍平、久保康隆
  • Organizer: 平成１８年度園芸学会秋季大会
• [Presentation] Determination of optimum temperature for long－term storage and analysis of ripening－related genes in ‘Rainbow Red’ kiwifruit. IX International Symposium on Kiwifruit. (2017)
  • Author(s): Tokiwa S., Mitalo O.W., Kasahara Y., Tosa Y., Kondo Y., Asiche W.O., Kataoka I., Suezawa K., Ushijima K., Nakano R., Kubo Y
  • Organizer: IX International Symposium on Kiwifruit.
  • Int'l Joint Research
• [Presentation] Ethylene induced ripening and low temperature modulated ripening in kiwifruit. IX International Symposium on Kiwifruit. (2017)
  • Author(s): Kubo Y., Asiche W.O., Mitalo O.W., Kasahara Y., Ushijima K., Nakano R.
  • Organizer: IX International Symposium on Kiwifruit.
  • Int'l Joint Research
• [Presentation] Characterization of ripening and associated gene expression in two kiwifruit cultivars at different storage temperatures. (2017)
  • Author(s): Mitalo O. W., Y. Tosa, Kasahara Y., Tokiwa S., Asiche W.O., Mworia E. G., Owino W. O., Ushijima K., Nakano R., Kubo Y
  • Organizer: The First All Africa Postharvest Congress and Exhibition.
  • Int'l Joint Research
• [Presentation] Comparison between ethylene and low temperature induced color change in Satsuma mandarin fruit through RNAseq analysis (2017)
  • Author(s): Mitalo O.W., Tosa Y., Kasahara Y., Tokiwa S., Kondo Y., Asiche O.W., Ushijima K., Nakano R., Kubo Y
  • Organizer: 平成２９年度園芸学会秋季大会
• [Presentation] Analysis of low temperature response in ‘Passe Crassane’ pear fruit using RNAseq (2017)
  • Author(s): Tosa Y., Mitalo O.W., Tokiwa S., Kondo Y., Ushijima K., Nakano R., Kubo Y.
  • Organizer: 平成２９年度園芸学会秋季大会
• [Presentation] Comparison of taste and flavor related compounds between ethylene dependent ripening and low temperature modulated ripening in Kosui kiwifruit. (2017)
  • Author(s): Tokiwa S., Kasahara Y., Tosa Y., Mitalo O.W., Kondo Y., Galis I., Kataoka I., Suezawa K., Nakano R., Ushijima K., Kubo Y
  • Organizer: 平成２９年度園芸学会秋季大会
• [Presentation] Ripening-related Genes are Differentially Regulated During Storage and On-vine Ripening in ‘Rainbow Red’ Kiwifruit. (2016)
  • Author(s): Mitalo O. W., Asiche W. O., Kasahara Y., Tosa Y., Tokiwa S., Ushijima K., Nakano R., Kubo Y.
  • Organizer: The Second Asian Horticultural Congress
  • Place of Presentation: Chengdu, China
  • Year and Date: 2016-09-26
• [Presentation] Low Temperature-Modulated Ripening in ‘Sanuki Gold’ and ‘Hayward’ Kiwifruit. (2016)
  • Author(s): Tosa Y., Mitalo O. W., Asiche W. O., Kasahara Y., Tokiwa S., Ushijima K., Nakano R., Kubo Y.
  • Organizer: The Second Asian Horticultural Congress
  • Place of Presentation: Chengdu, China
• [Presentation] Effects of Storage Temperature on Expression of Ripening- associated Genes in Kiwifruit. (2016)
  • Author(s): Kasahara Y., Asiche W. O., Mitalo O. W., Tosa Y., Tokiwa S., Ushijima K., Nakano R., Kubo Y.
  • Organizer: The Second Asian Horticultural Congress
  • Place of Presentation: Chengdu, China