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Development of new cultivars and MAS for useful traits in pear fruit using self-crossed F2 seedlings

Research Project

Project/Area Number 16H04874
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticultural science
Research InstitutionKyoto Prefectural University

Principal Investigator

ITAI AKIHIRO  京都府立大学, 生命環境科学研究科, 教授 (10252876)

Co-Investigator(Kenkyū-buntansha) 児玉 基一朗  鳥取大学, (連合)農学研究科(研究院), 教授 (00183343)
村山 秀樹  山形大学, 農学部, 教授 (40230015)
山本 俊哉  国立研究開発法人農業・食品産業技術総合研究機構, 本部, 所長・部門長・部長・研究管理役等 (60355360)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2018: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Keywords高密度連鎖地図 / 追熟性 / 病害罹病性 / リグニン / QTL / 種間雑種 / 肉質 / 園芸 / 果樹 / 育種 / ナシ / MAS / 園芸学 / MAS / 単為結実性 / 熟期
Outline of Final Research Achievements

We constructed a high-density genetic linkage map with GRAS-Di DNA markers using self-crossed interspecific F2 population between Pyrus communis and P. pyrifolia. The map converged into 17LGs corresponding to the pear chromosome number. The genetic locus for brown spot disease susceptibility was mapped to LG11. However, we could not identify the gene responsible for the trait. We conducted QTL analysis for the trait of non-ripening on the tree derive from European pear, but could not detect the QTL due to a small number of bearing seedlings. We also investigated the fruit lignin content of pear and relative species.

Academic Significance and Societal Importance of the Research Achievements

本研究は、セイヨウナシの重要病害として世界で問題となっているブラウンスポット病のマッピングに成功しており、原因遺伝子までの単離に到らなかったものの、今後の病害抵抗性を目的としたセイヨウナシ育種に伸展をもたらすものである。またナシ独特のざらざらした食感をもたらす石細胞の成分であるリグニン含量についての品種間差異について今回明らかにすることが出来た。今後の良食感をもたらすナシ育種の基盤となる。

Report

(5 results)
  • 2020 Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (27 results)

All 2020 2019 2018 2017 2016

All Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 10 results,  Open Access: 8 results,  Acknowledgement Compliant: 1 results) Presentation (16 results) (of which Int'l Joint Research: 2 results) Book (1 results)

  • [Journal Article] Analysis of Fruit Lignin Content, Composition, and Linkage Types in Pear Cultivars and Related Species2020

    • Author(s)
      Zhang Lumin、Kamitakahara Hiroshi、Murayama Hideki、Ohsako Takanori、Itai Akihiro
    • Journal Title

      Journal of Agricultural and Food Chemistry

      Volume: 68 Issue: 8 Pages: 2493-2505

    • DOI

      10.1021/acs.jafc.9b07396

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effect of exogenous GA4+7 and BA+CPPU treatments on fruit lignin and primary metabolites in Japanese pear “Gold Nijisseiki”2020

    • Author(s)
      Zhang Lumin、Kamitakahara Hiroshi、Sasaki Ryosuke、Oikawa Akira、Saito Kazuki、Murayama Hideki、Ohsako Takanori、Itai Akihiro
    • Journal Title

      Scientia Horticulturae

      Volume: 272 Pages: 109593-109593

    • DOI

      10.1016/j.scienta.2020.109593

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Genetic mapping of a locus controlling the intergeneric hybridization barrier between apple and pear2019

    • Author(s)
      Morimoto Takuya、Inaoka Maia、Banno Kiyoshi、Itai Akihiro
    • Journal Title

      Tree Genetics & Genomes

      Volume: 16 Issue: 1 Pages: 1-11

    • DOI

      10.1007/s11295-019-1397-7

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of QTLs associated with conversion of sucrose to hexose in mature fruit of Japanese pear.2018

    • Author(s)
      Nishio, S., T. Saito, S. Terakami, N. Takada, H. Kato, A. Itai and T. Saito.
    • Journal Title

      Plant Molecular Reporter

      Volume: 36 Issue: 4 Pages: 643-652

    • DOI

      10.1007/s11105-018-1106-y

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Ripening Characteristics and Pigment Changes in Russeted Pear Fruit in Response to Ethylene and 1-MCP2018

    • Author(s)
      Charoenchongsuk Nongluk、Matsumoto Daiki、Itai Akihiro、Murayama Hideki
    • Journal Title

      Horticulturae

      Volume: 4 Issue: 3 Pages: 22-22

    • DOI

      10.3390/horticulturae4030022

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genome-wide association study and genomic prediction using parental and breeding populations of Japanese pear (Pyrus pyrifolia Nakai)2018

    • Author(s)
      Minamikawa Mai F.、Takada Norio、Terakami Shingo、Saito Toshihiro、Onogi Akio、Kajiya-Kanegae Hiromi、Hayashi Takeshi、Yamamoto Toshiya、Iwata Hiroyoshi
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 11994-11994

    • DOI

      10.1038/s41598-018-30154-w

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Estimation of loss of genetic diversity in modern Japanese cultivars by comparison of diverse genetic resources in Asian pear (Pyrus spp.)2016

    • Author(s)
      Nishio, S., N. Takada, T. Saito, T. Yamamoto, H. Iketani
    • Journal Title

      BMC Genetics

      Volume: 17 Issue: 1 Pages: 81-81

    • DOI

      10.1186/s12863-016-0380-7

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of GA<sub>3+4</sub> and GA<sub>4+7</sub> Application Either Alone or Combined with Prohexadione-Ca on Fruit Development of Japanese Pear ‘Kosui’2016

    • Author(s)
      Ito, A., D. Sakamoto, A. Itai, T. Nishijima, N. Oyama-Okubo, Y. Nakamura, T. Moriguchi and I. Nakajima
    • Journal Title

      The Horticulture Journal

      Volume: 85 Issue: 3 Pages: 201-208

    • DOI

      10.2503/hortj.MI-107

    • NAID

      130005167406

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The global regulator LaeA controls biosynthesis of host-specific toxins, pathogenicity and development of Alternaria alternata pathotypes2016

    • Author(s)
      Takao, K., Akagi, Y., Tsuge, T., Harimoto, Y., Yamamoto, M., and Kodama, M.
    • Journal Title

      Journal of General Plant Pathology

      Volume: 82 Issue: 3 Pages: 121-131

    • DOI

      10.1007/s10327-016-0656-9

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Alternaria alternata apple pathotype (A. mali) causes black spot of European pear.2016

    • Author(s)
      Tanahashi, M., Nakano, T., Akamatsu, H., Kodama, M., Otani, H.and Osaki-Oka, K.
    • Journal Title

      European Journal of Plant Pathology

      Volume: 145 Issue: 4 Pages: 2627-2631

    • DOI

      10.1007/s10658-016-0866-1

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] ニホンナシ成熟果実における糖組成のゲノムワイドアソシエーション解析2020

    • Author(s)
      西尾聡悟, 齋藤寿広, 林武司, 白澤健太, 寺上伸吾, 高田教臣, 竹内由季恵, 加藤秀憲, 板井章浩
    • Organizer
      園芸学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ニホンナシ果実のスクロース含量に関与するDNAマーカーの開発2019

    • Author(s)
      佐藤みそら, 西尾聡悟, 寺上伸吾, 齋藤寿広, 山本俊哉, 村山秀樹, 板井章浩
    • Organizer
      園芸学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 自家および他家受粉が自家和合性品種`おさゴールド'の果実形質に及ぼす影響2019

    • Author(s)
      中島由葵, 山口俊春, 金高伸吾, 虎尾亮, 池田隆政, 村山秀樹, 平山隆志, 森泉, 松浦恭和, 板井章浩
    • Organizer
      園芸学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 新規重症果物アレルゲン Gibberellin-Regulated Protein の分布と発現解析2019

    • Author(s)
      成田宏史, 門間敬子, 岡崎史子, 森本拓也, 板井章浩
    • Organizer
      園芸学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] リンゴ属とナシ属の交雑(不)和合性を支配するゲノム領域の同定2019

    • Author(s)
      森本拓也, 稲岡麻衣亜, 伴野潔, 板井章浩
    • Organizer
      園芸学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] GAおよびCPPU処理がニホンナシ`ゴールド二十世紀'の果実形質, リグニンおよび一次代謝物含量に及ぼす影響2019

    • Author(s)
      章ルミン, 上高原浩, 及川彰, 斉藤和季, 村山秀樹, 大迫敬義, 板井章浩
    • Organizer
      園芸学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] セイヨウナシ果梗師管液のオミクス解析2019

    • Author(s)
      乙部葵, 廣瀬亮太郎, 及川彰, 斉藤和季, 森仁志, 村山秀樹, 森本拓也, 板井章浩
    • Organizer
      園芸学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ナシおよびナシ近縁品種の果実収穫期におけるリグニン含量, 組成および結合様式の解析2018

    • Author(s)
      章ルミン・上高原浩・大迫敬義・村山秀樹・板井章浩
    • Organizer
      園芸学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 遺伝的背景の異なるニホンナシ品種間交雑におけるみつ症感受性のQTL 解析2018

    • Author(s)
      尾形夏海、喜多晃一、山本俊哉、西谷千佳子、井上栄一、市毛秀則
    • Organizer
      園芸学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ‘ゴールド二十世紀’と‘Alexandrine Douillard’の果実生育期におけるリグニン含量および組成の解析.2017

    • Author(s)
      章 ルミン、上高 原浩、大迫 敬義、村山 秀樹、板井 章浩
    • Organizer
      園芸学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ニホンナシの複数育種集団を利用した、ゲノムワイド関連解析とゲノミックセレクションの予測精度評価2017

    • Author(s)
      南川舞、寺上伸吾、高田教臣、齋藤寿広、小野木章雄、鐘ケ江弘美、林武司、山本俊哉、岩田洋佳
    • Organizer
      園芸学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 二ホンナシ多型情報を用いたハプロタイプブロックの検出2016

    • Author(s)
      田中剛、小貫律子、寺上伸吾、浦崎直也、太郎良和彦、護得久聡子、正田守幸、山本俊哉、伊藤剛
    • Organizer
      日本育種学会
    • Related Report
      2016 Annual Research Report
  • [Presentation] ニホンナシにおける網羅的なトランスクリプトーム配列決定2016

    • Author(s)
      小林正明、中村幸乃、西谷千佳子、山本俊哉、矢野健太郎
    • Organizer
      日本育種学会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Effects of 1-Methylcyclopropene and Ethylene Treatment on Ripening Characteristics of the ‘Silver Bell’ Pears2016

    • Author(s)
      B. Nugraha, N. Bintoro, J. Nugroho, A. Itai, and H. Murayama.
    • Organizer
      The 2nd International Symposium on Agricultural and Biosystem Engineering -ISABE-
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ナシ果実の成熟エチレン生成におけるシステムIからIIへの移行関連遺伝子の探索2016

    • Author(s)
      藤井美希・羽生剛・村山秀樹・児玉基一朗・板井章浩
    • Organizer
      園芸学会
    • Related Report
      2016 Annual Research Report
  • [Presentation] The evolution of mycotoxin/phytotoxin biosynthesis and virulence in the plant pathogenic fungus Alternaria alternata.2016

    • Author(s)
      Kodama, M., Akagi, Y. and Tsuge, T.
    • Organizer
      International Symposium of Mycotoxicology
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Book] フローサイトメトリー Q&A2017

    • Author(s)
      板井章浩 (戸村道夫編集)
    • Total Pages
      312
    • Publisher
      羊土社
    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2022-01-27  

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