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Exploration for tomato lines resistant against root parasitic plants using a large mutagenized population

Research Project

Project/Area Number 16H04875
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticultural science
Research InstitutionOsaka Prefecture University

Principal Investigator

Aoki Koh  大阪府立大学, 生命環境科学研究科, 教授 (30344021)

Research Collaborator Saito Junna  
Miyawaki Reika  
Ekawa Minako  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2018: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywords寄生植物 / トマト / 発芽後抵抗性 / Phelipanche / 抵抗性 / 野生近縁種 / Phelipanche aegyptiaca / Solanum pennellii / Orobanche / 抵抗性系統 / 変異体 / Cuscuta / 細胞壁 / 維管束 / 突然変異誘発系統
Outline of Final Research Achievements

We explored new resistance of tomato against a root parasitic plant, Phelipanche aegyptiaca, which causes serious damage to tomato production, by using EMS-mutagenized population, fixed mutant lines and tomato wild relative species. Among these variations, P. aegyptiaca showed significantly lower parasitic rate to one wild relative species, Solanum pennellii. This lead us to use established introgression lines of S. pennellii x S. lycopersicum, consisting of 76 lines, to identify genomic region responsible for the post-germination resistance. Analysis of introgression lines revealed that putative responsible genes are dispersed in 11 genomic regions. We are currently attempting to identify individual genes responsible for the resistance. Additionally, we tested ccd8 mutant defective in strigolactone biosynthesis. We verified that parasitic rate of P. aegyptiaca to ccd8 mutant actually lower than to normal S. lycopersicum.

Academic Significance and Societal Importance of the Research Achievements

根寄生植物によるトマトの被害は世界中で大きな問題となっている。これに抵抗性を示すトマト系統は、根寄生植物の発芽刺激物質であるストリゴラクトン生合成欠損系統に限られていた。本研究では、従来の抵抗性とは異なり、発芽後寄生植物がトマトに付着した後の段階で発揮される抵抗性を探索し、野生近縁種S. pennelliiの中にその性質があることを見出し原因遺伝子同定への歩を進めた。この発芽後抵抗性は、発芽を抑制することによる抵抗性と併せて、根寄生植物へのより強固な抵抗性保持につながると考えられ、世界各地でのトマト生産を助けるものとなると思われる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (18 results)

All 2019 2018 2017 2016 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (12 results) (of which Int'l Joint Research: 5 results) Remarks (2 results)

  • [Journal Article] Low Infection of Phelipanche aegyptiaca in Micro-Tom Mutants Deficient in CAROTENOID CLEAVAGE DIOXYGENASE 82018

    • Author(s)
      Hasegawa Shoko、Tsutsumi Takuya、Fukushima Shunsuke、Okabe Yoshihiro、Saito Junna、Katayama Mina、Shindo Masato、Yamada Yusuke、Shimomura Koichiro、Yoneyama Kaori、Akiyama Kohki、Aoki Koh、Ariizumi Tohru、Ezura Hiroshi、Yamaguchi Shinjiro、Umehara Mikihisa
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 19 Issue: 9 Pages: 2645-2645

    • DOI

      10.3390/ijms19092645

    • NAID

      120007133661

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Differentiation of vascular elements in haustoria of Cuscuta japonica.2018

    • Author(s)
      Kohki Shimizu, Koh Aoki
    • Journal Title

      Plant Signal Behav.

      Volume: 13 Issue: 3 Pages: e1445935-e1445935

    • DOI

      10.1080/15592324.2018.1445935

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Organization of vascular cells in the haustorium of the parasitic flowering plant Cuscuta japonica.2018

    • Author(s)
      Kohki Shimizu, Akitaka Hozumi, Koh Aoki
    • Journal Title

      Plant Cell Physiol.

      Volume: 59 Issue: 4 Pages: 715-723

    • DOI

      10.1093/pcp/pcx197

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phloem-Conducting Cells in Haustoria of the Root-Parasitic Plant Phelipanche aegyptiaca Retain Nuclei and Are Not Mature Sieve Elements.2017

    • Author(s)
      Ekawa M, Aoki K
    • Journal Title

      Plants (Basel)

      Volume: 6 Issue: 4 Pages: 60-60

    • DOI

      10.3390/plants6040060

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 寄生植物と宿主植物の相互作用における細胞内膜輸送の関与2019

    • Author(s)
      宮脇礼佳、青木考
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] トマトIntrogression linesを用いた根寄生植物Phelipanche aegyptiacaの発芽後抵抗性を示す遺伝子の同定2019

    • Author(s)
      斉藤純奈、青木考
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Exploration of new resistance of tomato to a root parasitic plant, Phelipanche aegyptiaca.2018

    • Author(s)
      Junna Saito, Koh Aoki
    • Organizer
      The 15th Japan Solanaceae Consortium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Exploration of new resistance of tomato to a root parasitic plant, Phelipanche aegyptiaca.2018

    • Author(s)
      Junna Saito, Koh Aoki
    • Organizer
      The 15th Solanaceae Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Connecting interface of tomato with a root parasitic plant Phelipanche aegyptiaca can be a potential target for post-haustorial resistance.2018

    • Author(s)
      Minako Ekawa, Junna Saito, Koh Aoki
    • Organizer
      The 15th Solanaceae Conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 寄生植物吸器における通導細胞分化2018

    • Author(s)
      青木考,江川美菜子,清水皇稀,藤原大輝,ベラ スブハンカール
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 茎寄生植物Cuscuta japonicaの吸器における維管束分化関連遺伝子の発現2018

    • Author(s)
      清水皇稀、穂積亮敬、青木考
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Symplasmic continuity between Orobanche aegyptiaca and the host2017

    • Author(s)
      江川美菜子、青木考
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学、鹿児島
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Expression of genes involved in vascular differentiation in haustorium of Cuscuta japonica.2017

    • Author(s)
      Shimizu K, Hozumi A, Aoki K、
    • Organizer
      World Congress on Parasitic Plants 14
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Apoplastic and symplastic interactions between parasitic plants and host plants.2017

    • Author(s)
      Aoki K, Hozumi A, Ekawa M, Shimizu K, Yokoyama R, Nishitani K
    • Organizer
      World Congress on Parasitic Plants 14
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 根寄生植物Orobanche aegyptiacaと宿主植物間のシンプラスミックな接続形成2017

    • Author(s)
      江川美菜子,青木考
    • Organizer
      第35回日本植物細胞分子生物学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Improvement of the transformation method of root parasitic plant Orobanche aegyptiaca2016

    • Author(s)
      江川美菜子、青木考
    • Organizer
      第34回日本植物細胞生物学会
    • Place of Presentation
      信州大学繊維学部、上田
    • Year and Date
      2016-09-01
    • Related Report
      2016 Annual Research Report
  • [Remarks] 機能ゲノム科学研究グループ

    • URL

      https://www.kohaokilab.com/

    • Related Report
      2018 Annual Research Report 2016 Annual Research Report
  • [Remarks] 機能ゲノム科学研究室

    • URL

      https://www.kohaokilab.com/

    • Related Report
      2017 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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