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Novel aspects of plant priming and its use for crop breeding

Research Project

Project/Area Number 16H04879
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant protection science
Research InstitutionHokkaido University

Principal Investigator

Nakahara Kenji  北海道大学, 農学研究院, 講師 (90315606)

Co-Investigator(Kenkyū-buntansha) 山田 哲也  北海道大学, 農学研究院, 講師 (70374618)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2019: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2018: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywords全身獲得抵抗性 / カルモジュリン様タンパク質 / ウイルス防御機構 / RNAサイレンシング抑制タンパク質 / オートファジー / 転写促進 / 植物自然免疫 / ゲノム編集 / ウィルス / 病理学 / 植物 / シグナル伝達 / ストレス / ストレス応答 / 自然免疫 / プライミング / RNA / ウイルス
Outline of Final Research Achievements

We found that 6 calmodulin-like proteins (CML) and endogenous factors that binding to the CMLs interact with virus RNA silencing suppressors and are involved in systemic acquired resistance. Based on the results obtained in this study, we hypothize the molecular mechanism involving these CMLs and endogenous factors unldelying systemic acquired resistance. Moreover, genome editing the gene that were selected according to the results of this study confer antiviral resistance on tomato.

Academic Significance and Societal Importance of the Research Achievements

全身獲得抵抗性は繰り返し襲来する病原体感染により強い抵抗性を示すために植物が獲得した防御機構で100年前から知られているが、そのメカニズムは未だ不明の点が多い。本研究でこの全身獲得抵抗性に関わる新たな因子を同定し、それらによる分子メカニズムを解明した。これらの成果は、学術的に重要なだけでなく、全身獲得抵抗性を病害防除や抵抗性育種に活用するための基礎的な知見として農産物生産への貢献も期待出来る。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (25 results)

All 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (8 results) Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 1 results,  Acknowledgement Compliant: 3 results) Presentation (4 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] Queen's University/University of Toronto(カナダ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] 国立台湾大学(その他の国・地域)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] クイーンズ大学(カナダ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] テネシー州立大学(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] 國立臺灣大学(その他の国・地域 台湾)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] クイーンズ大学(カナダ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] テネシー州立大学(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] 国立台湾大学(台湾)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] First report of Botrytis porri causing Botrytis leaf blight on leek in Japan2020

    • Author(s)
      Misawa T.、Ueno R.、Kurose D.、Nakahara K.S.
    • Journal Title

      New Disease Reports

      Volume: 41 Issue: 1 Pages: 19-19

    • DOI

      10.5197/j.2044-0588.2020.041.019

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Intracellular proliferation of clover yellow vein virus is unaffected by the recessive resistance gene cyv1 of Pisum sativum2020

    • Author(s)
      Taninaka Yosuke、Nakahara Kenji S.、Hagiwara‐Komoda Yuka
    • Journal Title

      Microbiology and Immunology

      Volume: 64 Issue: 1 Pages: 76-82

    • DOI

      10.1111/1348-0421.12755

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Precise Exchange of the Helper-Component Proteinase Cistron Between Soybean mosaic virus and Clover yellow vein virus: Impact on Virus Viability and Host Range Specificity2020

    • Author(s)
      Wang Y.、Xu W.、Abe J.、Nakahara K. S.、Hajimorad M. R.
    • Journal Title

      Phytopathology

      Volume: 110 Issue: 1 Pages: 206-214

    • DOI

      10.1094/phyto-06-19-0193-fi

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Recessive resistance governed by a major quantitative trait locus restricts clover yellow vein virus in mechanically but not graft-inoculated cultivated soybeans2019

    • Author(s)
      Abe Junya、Wang Yongzhi、Yamada Tetsuya、Sato Masako、Ono Takuya、Atsumi Go、Abe Jun、Hajimorad M. R.、Nakahara Kenji Suto
    • Journal Title

      Molecular Plant-Microbe Interactions

      Volume: 32 Issue: 8 Pages: 1026-1037

    • DOI

      10.1094/mpmi-12-18-0331-r

    • NAID

      120006734169

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] CRISPR/Cas9-mediated editing of genes encoding rgs-CaM-like proteins in transgenic potato plants2018

    • Author(s)
      Zhila Osmani、Shinnosuke Jin、Masafumi Mikami、Masaki Endo、Hiroki Atarashi、Kaien Fujino、Tetsuya Yamada、Kenji S. Nakahara
    • Journal Title

      Methods in Molecular Biology

      Volume: in press

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Paired immunities that impose trade-offs on viruses secure durability of the immunities in plants2017

    • Author(s)
      中原 健二
    • Journal Title

      生化学

      Volume: 89 Issue: 3 Pages: 436-440

    • DOI

      10.14952/SEIKAGAKU.2017.890436

    • NAID

      120006360100

    • ISSN
      0037-1017
    • Year and Date
      2017-06-25
    • Related Report
      2017 Annual Research Report
  • [Journal Article] rgs-CaM Detects and Counteracts Viral RNA Silencing Suppressors in Plant Immune Priming.2017

    • Author(s)
      Jeon EJ, Tadamura K, Murakami T, Inaba J-I, Kim BM, Sato M, Atsumi G, Kuchitsu K, Masuta C, Nakahara KS
    • Journal Title

      Journal of Virology

      Volume: 91 Issue: 19 Pages: 00761-17

    • DOI

      10.1128/jvi.00761-17

    • NAID

      120006416011

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microperforated leaf blotting on polyvinylidene difluoride and nylon membranes to analyze spatial distribution of endogenous and viral gene expression in plant leaves2016

    • Author(s)
      Murakami, T., Tayama, R., Nakahara, K.S
    • Journal Title

      Journal of General Plant Pathology

      Volume: 82 Issue: 5 Pages: 254-260

    • DOI

      10.1007/s10327-016-0671-x

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Trade-offs for viruses in overcoming innate immunities in plants2016

    • Author(s)
      Miyashita, Y., Atsumi, G., Nakahara, K.S
    • Journal Title

      Molecular Plant-Microbe Interactions

      Volume: 29 Issue: 8 Pages: 595-598

    • DOI

      10.1094/mpmi-05-16-0103-cr

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] P3N-PIPO, a frameshift product from P3, pleiotropically determines the virulence of clover yellow vein virus in both resistant and susceptible peas.2016

    • Author(s)
      Atsumi, G., Suzuki, H., Miyashita, Y., Choi, S.H., Hisa, Y., Rihei, S., Shimada, R., Jeon, E.J., Abe, J., Nakahara, K.S., Uyeda, I
    • Journal Title

      Journal of Virology

      Volume: 90 Issue: 16 Pages: 7388-7404

    • DOI

      10.1128/jvi.00190-16

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] Interaction of Arabidopsis calmodulin-like proteins with the protein 2b, an RNA silencing suppressor of cucumber mosaic virus2019

    • Author(s)
      Nakahara, K., H. Teresinski, M. Suto, S. Jin, W. Snedden
    • Organizer
      Plant Canada 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] キュウリモザイクウイルス感染シロイヌナズナにおけるカルモジュリン様タンパク質37, 38, 39欠損の影響2018

    • Author(s)
      神 慎之介、佐藤雅子、須藤深雪、中原健二
    • Organizer
      日本植物病理学会北海道部会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ダイズとツルマメ間の組換え自殖系統におけるクローバ葉脈黄化ウイルスに対する抵抗性の再評価2018

    • Author(s)
      小野卓哉、山田哲也、中原健二
    • Organizer
      日本植物病理学会北海道部会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ダイズ栽培化で選抜されたかもしれないクローバ葉脈黄化ウイルス抵抗性について2018

    • Author(s)
      中原健二・山田哲也
    • Organizer
      第13回 植物ウイルス病研究会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Book] CRISPR/Cas9-Mediated Editing of Genes Encoding rgs-CaM-like Proteins in Transgenic Potato Plants, Antiviral Resistance in Plants, Methods in Molecular Biology2019

    • Author(s)
      Osmani Zhila、Jin Shinnosuke、Mikami Masafumi、Endo Masaki、Atarashi Hiroki、Fujino Kaien、Yamada Tetsuya、Nakahara Kenji S.
    • Publisher
      Humana, New York, NY
    • ISBN
      9781493996353
    • Related Report
      2019 Annual Research Report
  • [Remarks] プレスリリース「ウイルスの強毒化を防ぐエンドウの仕組みを発見」(中原 健二 講師)

    • URL

      http://www.agr.hokudai.ac.jp/news/2016/06/post-677.html

    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] ウイルス抵抗性植物及びその作成方法2017

    • Inventor(s)
      新子泰規、中原健二、山田哲也、増田税
    • Industrial Property Rights Holder
      新子泰規、中原健二、山田哲也、増田税
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2022-08-19  

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