Analysis on the mechanism for the yellow induction on tobacco by cucumber mosaic virus and its satellite

• Project/Area Number: 16H04880
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant protection science
• Research Institution: Hokkaido University
• Principal Investigator: Masuta Chikara 北海道大学, 農学研究院, 教授 (60281854)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000); Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2017: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2016: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: RNAサイレンシング / キュウリモザイクウイルス / サテライトRNA / 病徴誘導 / 光合成 / サイレンシング / 黄化

Outline of Final Research Achievements

Tobacco plants infected with Y-satellite RNA (Y-sat) in the presence of cucumber mosaic virus (CMV) show yellowing symptoms due to RNA silencing (RS) against the host ChlI gene mRNA. For this symptom induction, the secondary siRNA amplification by RDR6 in the RS pathway was found to be important. The yellow tobacco was found to be more tolerant to drought stress than the healthy control although ChlI is also involved in stoma regulation. In addition, the yellow color was demonstrated to be advantageous for aphid transmission of CMV+Y-sat and thus for their survival.

Academic Significance and Societal Importance of the Research Achievements

植物におけるウイルス病の病徴発現メカニズムを分子レベルで理解できれば、ウイルス病の防除対策の立案に大きく貢献できる。本研究で、一見偶然にみえるウイルス感染によるタバコの黄化が、実は、アブラムシを介したウイルス伝搬に有利な状況を作り出すウイルス側の巧緻な戦略であることが明らかになった。このような研究成果は、植物とウイルスの闘い（共生）と生き残り戦略の理解につながり、現在我々が食料として利用している作物の栽培を最大限に高度化するための基盤知見として高い学術的意義を有するものと考える。

Research Products

• [Journal Article] Transcriptional silencing of 35S driven-transgene is differentially determined depending on promoter methylation heterogeneity at specific cytosines in both plus-and minus-sense strands. (2019)
  • Author(s): Matsunaga, W., Shimura, H., Shirakawa, S., Isoda, R., Inukai, T., Matsumura, T., and Masuta, C.
  • Journal Title: BMC Plant Biology Volume: 19 Issue: 1 Pages: 24-24
  • DOI: 10.1186/s12870-019-1628-y
  • Peer Reviewed / Open Access
• [Journal Article] Rapid transient protein production by the coat protein-deficient cucumber mosaic virus vector: non-packaged CMV system, NoPaCS. (2018)
  • Author(s): Fukuzawa N., Masuta, C., and Matsumura, T.
  • Journal Title: Plant Cell Reports Volume: 37 Issue: 11 Pages: 1513-1522
  • DOI: 10.1007/s00299-018-2322-5
  • Peer Reviewed
• [Journal Article] Allexiviruses may have acquired insert sequences between the CP and CRP genes to change the translation reinitiation strategy of CR. (2018)
  • Author(s): Yoshida, N, Shimura, H., and Masuta, C.
  • Journal Title: Archives of Virology Volume: 163 Issue: 6 Pages: 1419-1427
  • DOI: 10.1007/s00705-018-3749-2
  • Peer Reviewed
• [Journal Article] Increased susceptibility of potato to Rhizoctonia diseases in Potato leafroll virus-infected plants (2017)
  • Author(s): Ito Mai、Meguro-Maoka Ayano、Maoka Tetsuo、Akino Seishi、Masuta Chikara
  • Journal Title: Journal of General Plant Pathology Volume: 83 Issue: 3 Pages: 169-172
  • DOI: 10.1007/s10327-017-0707-x
  • Peer Reviewed
• [Journal Article] Construction of a system for the strawberry nursery production towards elimination of latent infection of anthracnose fungi by a combination of PCR and micro tube-hybridization (PCR-MTH) (2017)
  • Author(s): Furuta, K., Nagashima, S., Inukai, T., and Masuta, C.
  • Journal Title: Plant Pathology Journal Volume: 33 Issue: 1 Pages: 80-86
  • DOI: 10.5423/ppj.nt.05.2016.0132
  • Peer Reviewed
• [Journal Article] rgs-CaM Detects and Counteracts Viral RNA Silencing Suppressors in Plant Immune Priming. (2017)
  • Author(s): Jeon EJ, Tadamura K, Murakami T, Inaba J-I, Kim BM, Sato M, Atsumi G, Kuchitsu K, Masuta C, Nakahara KS
  • Journal Title: Journal of Virology Volume: 91 Issue: 19 Pages: 00761-17
  • DOI: 10.1128/jvi.00761-17
  • NAID: 120006416011
  • Peer Reviewed
• [Journal Article] Interaction between Cucumber mosaic virus 2b protein and plant catalase induces a specific necrosis in association with proteasome activity (2017)
  • Author(s): Murota, K., Shimura, H., Takeshita, M. and Masuta, C.
  • Journal Title: Plant Cell Report Volume: 36 Issue: 1 Pages: 37-47
  • DOI: 10.1007/s00299-016-2055-2
  • Peer Reviewed / Open Access
• [Journal Article] Virus-induced gene silencing (VIGS) in <i>Lilium leichtlinii</i> using the <i>Cucumber mosaic virus</i> vector (2016)
  • Author(s): Keisuke Tasaki, Hiroyuki Terada, Chikara Masuta, Masumi Yamagishi
  • Journal Title: Plant Biotechnology Volume: 33 Issue: 5 Pages: 373-381
  • DOI: 10.5511/plantbiotechnology.16.1018a
  • NAID: 130005188239
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Ascorbic acid accumulates as a defense response to Turnip mosaic virus in resistant Brassica rapa cultivars. (2016)
  • Author(s): ujiwara, A., Togawa, S., Hikawa, T., Matsuura, H., Masuta, C. and Inukai, T.
  • Journal Title: Journal of Experimental Botany Volume: 67 Issue: 14 Pages: 4391-4402
  • DOI: 10.1093/jxb/erw223
  • NAID: 120005853073
• [Journal Article] Aquaporin-mediated long-distance polyphosphate translocation directed towards the host in arbuscular mycorrhizal symbiosis: application of virus-induced gene silencing (2016)
  • Author(s): Kikuchi, Y., Hijikata, N., Ohtomo, R., Handa, Y., Kawaguchi, M., Saito, K., Masuta, C., Ezawa, T
  • Journal Title: New Phytologist Volume: 211 Issue: 4 Pages: 1202-1208
  • DOI: 10.1111/nph.14016
  • NAID: 120006343690
  • Peer Reviewed
• [Journal Article] Chemical Promotion of Endogenous Amount of ABA in Arabidopsis thaliana by a Natural Product, Theobroxide. (2016)
  • Author(s): Yamashita, Y., Ota, M., Inoue, Y., Hasebe, Y., Okamoto, M., Inukai, T., Masuta, C., Sakihama, Y., Hasidoko, Y., Kojima, M., Sakakibara, H., Inage, Y., Takahashi, K., Yoshihara, T., and Matsuura H.
  • Journal Title: Plant Cell and Physiology Volume: 57 Issue: 5 Pages: 986-999
  • DOI: 10.1093/pcp/pcw037
  • Peer Reviewed
• [Presentation] A technique to reduce DNA methylation in a sequence-specific manner by using a ribozyme-expressing cucumber mosaic virus vector. (2018)
  • Author(s): Wataru Matsunaga, Reika Isoda, Tsuyoshi Inukai, Takeshi Matsumura and Chikara Masuta
  • Organizer: International Congress of Plant Pathology 2018
  • Int'l Joint Research
• [Presentation] ウイルスベクターから供給したリボザイムは配列特異的に脱メチル化を誘導する (2018)
  • Author(s): 礒田玲華，松永航，白川千里，犬飼剛，松村健，増田税
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] タマネギ萎縮ウイルス日本分離株のHC-Proタンパク質はN末端を欠失している (2018)
  • Author(s): 青木那奈，高橋春南，礒田玲華，増田税
  • Organizer: 日本植物病理学会北海道部会
• [Presentation] キュウリモザイクウイルスサテライトRNAが感染したベンタミアーナにおいて観察される乾燥耐性の発現機構 (2017)
  • Author(s): 増山翔太・林勇作・松浦英幸・増田税
  • Organizer: 日本植物病理学会北海道部会
• [Presentation] ウイルスフリーニンニク鱗片の大きさと糖度の均一性について (2017)
  • Author(s): 高橋春南・両國香・増田税
  • Organizer: 日本植物病理学会北海道部会
• [Presentation] PH01-type fungal transporter is responsible for phosphate export to the host in arbuscular mycorrhizal symbiosis (2017)
  • Author(s): Hayato Maruyama, Kaede Yokoyama, Yusuke Kikuchi, Natsuki Nakanishi, Ayumi Abe, Teruo Sone, Katsuharu Saito, Chikara Masuta and Tatsuhiro Ezawa
  • Organizer: 3rd International Molecular Mycorrhiza Meeting (France)
  • Int'l Joint Research
• [Presentation] PH01-type transporter of arbuscular mycorrhizal fungi mediates phosphate export in the plant-fungal interface (2017)
  • Author(s): Hayato Maruyama, Kaede Yokoyama, Yusuke Kikuchi, Natsuki Nakanishi, Ayumi Abe, Teruo Sone, Katsuharu Saito, Chikara Masuta and Tatsuhiro Ezawa
  • Organizer: 9th International Conference on Mycorhiza (Czech Republic)
  • Int'l Joint Research
• [Presentation] Rhizophagus clarus-ウイルス間相互作用に駆動される酸性土壌適応：大陸間隔離株の共通ウイルスは酸性土壌において正の選択圧を受ける (2016)
  • Author(s): 鈴木芽以、中西夏輝、丸山隼人、俵谷圭太郎、増田税、江沢辰広
  • Organizer: 菌根研究会2016年大会
  • Place of Presentation: 千葉大学西千葉キャンパス（千葉県千葉市）
  • Year and Date: 2016-12-10
• [Presentation] キュウリモザイクウイルス2bタンパク質によるゲノムワイドなメチル化に関するin silico解析 (2016)
  • Author(s): 白川千里、外川靖子、犬飼剛、増田税
  • Organizer: 日本植物病理学会北海道部会
  • Place of Presentation: かでる2・7（北海道札幌市）
  • Year and Date: 2016-10-19
• [Presentation] 茎頂培養によりウイルスフリー化した八紘ニンニクの収量・品質の変化 (2016)
  • Author(s): 両國香、志村華子、増田税
  • Organizer: 日本植物病理学会北海道部会
  • Place of Presentation: かでる2・7（北海道札幌市）
  • Year and Date: 2016-10-19
• [Presentation] Cucumber mosaic virus感染後のトウガラシにおけるアスコルビン酸代謝関連遺伝子群のエピジェネティクス発現制御 (2016)
  • Author(s): 妙中駿之、古谷未咲、松村健、増田税
  • Organizer: 日本植物病理学会北海道部会
  • Place of Presentation: かでる2・7（北海道札幌市
  • Year and Date: 2016-10-19
• [Presentation] ウイルス外被タンパク質遺伝子を欠失させたCMVベクターを用いたアグロインフェクション法 (2016)
  • Author(s): 福澤徳穂、佐藤敦子、増田税、松村健
  • Organizer: 日本植物細胞分子生物学会
  • Place of Presentation: 信州大学繊維学部（長野県上田市）
  • Year and Date: 2016-09-01
• [Presentation] Cucumber mosaic virus 2b protein alters the plant epigenomes (2016)
  • Author(s): Yasuko TOGAWA, Raku SAITO, Senri SHIRAKAWA, Chikara MASUTA
  • Organizer: The Plant Biotech 2016
  • Place of Presentation: Queen's University in Kingston, ON, Canada
  • Year and Date: 2016-06-19
• [Book] Metagenomic analyses of the viruses detected in mycorrhizal funji and their host orchid. In Methods Mol Biol. (2018)
  • Author(s): Methods Mol Biol. 2018;1746:161-172. doi: 10.1007/978-1-4939-7683-6_12. Metagenomic Analyses of the Viruses Detected in Mycorrhizal Fungi and Their Host Orchid. Shimura H1, Masuta C2, Koda Y2.
  • Total Pages: 12
  • Publisher: The Humana Press Inc.
• [Patent(Industrial Property Rights)] 植物における標的DNAのメチル化を抑制する方法 (2018)
  • Inventor(s): 増田税・犬飼剛・松永航・磯田玲華・松村健・厚見剛
  • Industrial Property Rights Holder: 増田税・犬飼剛・松永航・磯田玲華・松村健・厚見剛
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2018-139316
  • Filing Date: 2018