Mechanism of resistance to glyphosate and glufosinate in weeds

• Project/Area Number: 16H04884
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant protection science
• Research Institution: Kyoto University
• Principal Investigator: Tohru TOMINAGA 京都大学, 農学研究科, 教授 (10135551)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000); Fiscal Year 2018: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2016: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
• Keywords: 除草剤抵抗性 / グリホサート / ネズミムギ / オヒシバ / ヒメムカシヨモギ / オオアレチノギク / グルホシネート / 非選択性除草剤 / 抵抗性 / 抵抗性機構

Outline of Final Research Achievements

Both glyphosate and glufosinate are non-selective herbicide widely used in the world. Glyphosate resistant Lolium multiflorum, Eleusine indica, Conyza canadensis and C. sumatrensis are reported in Japan. Among them, L. multiflorum from Shizuoka was also resistant to glufosinate. The glyphosate resistance of L. multiflorum from Shizuoka, E. indica from Okayama and Okinawa, C. canadensis from Kyoto and C. sumatrensis from Gifu was endowed by non-target site mechanisms. The resistance of E. indica from Nara and Mie was endowed by target site mechanisms.

Academic Significance and Societal Importance of the Research Achievements

雑草の除草剤抵抗性機構は、作用点抵抗性と非作用点抵抗性に大別される。抵抗性機構によって抵抗性の程度や多剤抵抗性の有無が異なるため、抵抗性機構を明らかにすることは、除草剤抵抗性雑草の合理的な防除計画策定に極めて重要である。本研究では、ネズミムギ、オヒシバ、ヒメムカシヨモギ及びオオアレチノギクのグリホサート抵抗性機構を解析した。静岡産ネズミムギの非作用点抵抗性機構に関して、液胞隔離が関与している可能性を示した。オヒシバの抵抗性に関しては、集団によって抵抗性機構が異なることを示した。

Research Products

• [Journal Article] Non-target-site mechanism of glyphosate resistance in Italian ryegrass (Lolium multiflorum) (2018)
  • Author(s): Kurata Kohei、Niinomi Yuki、Shimono Yoshiko、Miyashita Masahiro、Tominaga Tohru
  • Journal Title: Weed Biology and Management Volume: 18 Issue: 3 Pages: 127-135
  • DOI: 10.1111/wbm.12156
  • Peer Reviewed
• [Presentation] グリホサート抵抗性ネズミムギの非作用点抵抗性機構 (2018)
  • Author(s): 倉田康平・下野嘉子・岩上哲史・宮下正弘・冨永達
  • Organizer: 日本雑草学会第57回大会
• [Presentation] 日本におけるグリホサート抵抗性雑草の出現とそれらの抵抗性機構 (2017)
  • Author(s): 倉田康平・永井絵理・下野嘉子・岩上哲史・冨永達
  • Organizer: 日本雑草学会第56回大会
  • Place of Presentation: 宮崎県宮崎市シーガイアコンベンションセンター
  • Year and Date: 2017-04-15
• [Presentation] Non-target site resistance to glyphosate in Lolium multiflorum in Japan (2017)
  • Author(s): Kohei Kurata, Yoshiko Shimono, Satoshi Iwakami, Masahiro Miyashita, Tohru Tominaga
  • Organizer: The 26th Asian-Pacific Weed Science Society Congress
  • Int'l Joint Research
• [Presentation] Mechanism of glyphosate resistance of Lolium multiflorum in Japan (2017)
  • Author(s): Kohei Kurata, Yuki Niinomi, Yoshiko Shimono, Masahiro Miyashita, Tohru Tominaga
  • Organizer: Global Herbicide Resistance Challenge
  • Int'l Joint Research