Studies on host switching mechanism of phytoplasma infecting plants and insects
| Project/Area Number |
16H04885
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant protection science
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| Research Institution | Hosei University |
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Principal Investigator |
Oshima Kenro 法政大学, 生命科学部, 教授 (00401183)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Fiscal Year 2019: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Keywords | ファイトプラズマ / ゲノム / 宿主 / 媒介昆虫 / ホストスイッチング / シグマ因子 / 植物病原細菌 |
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| Outline of Final Research Achievements |
Phytoplasma is a phytopathogenic bacterium that spreads infection by host-switching horizontally translocating between plant and insect hosts. In this study, we analyzed the host switching mechanism of phytoplasma. First, we analyzed transcriptional regulation of sigma factor RpoD involved in host switching, identified the promoter sequence, and clarified that it is involved in transcriptional regulation of various genes. Next, to analyze host-host interaction in trees, we identified phytoplasma that causes yellowing diseases of Elaeocarpus zollingeri. Furthermore, we analyzed the crystal structure of the secreted protein PHYL1 that functions during plant infection and identified the amino acid sequences important for its function.
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| Academic Significance and Societal Importance of the Research Achievements |
ファイトプラズマは植物に寄生して病気を引き起こす病原細菌である。イタリアのリンゴ生産だけでも年間1億ユーロの被害が出ているが、ファイトプラズマ病には特効薬が無く、防除はとりわけ困難である。ファイトプラズマは昆虫を介したホストスイッチングによって植物から植物へと伝搬される。本研究ではホストスイッチング機構の一端を明らかにしたが、その鍵となる因子はファイトプラズマ病予防の新たな標的となる可能性がある。また、ホストスイッチングはファイトプラズマ独特の生存システムであることから、これを標的とする薬剤は環境への影響が少ないことが予想され、環境保全型の薬剤開発に寄与できるという点に学術的意義がある。
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Report
(5 results)
Research Products
(36 results)
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[Journal Article] Structural analysis of phyllogen, a phyllody-inducing effector, revealed the importance of two conserved-helices.2019
Author(s)
Kitazawa, Y., Iwabuchi, N., Maejima, K., Miyatake, H., Nishikawa, M., Tokuda, R., Oshima, K., Yasuyuki, Y. & Namba, S.
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Journal Title
Phytopathogenic Mollicutes
Volume: 9
Issue: 1
Pages: 127-128
DOI
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Peer Reviewed
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[Journal Article] Phytoplasma-conserved phyllogen proteins induce phyllody across the Plantae by degrading floral MADS domain proteins2017
Author(s)
Kitazawa, Y., Iwabuchi, N., Himeno, M., Sasano, M., Koinuma, H., Nijo, T., Tomomitsu, T., Yoshida, T., Okano, Y., Yoshikawa, N., Maejima, K., Oshima, K. and Namba, S.
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Journal Title
Journal of Experimental Botany
Volume: 印刷中
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Peer Reviewed
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[Presentation] Functional analysis of RpoD, a principle sigma factor conserved among phytoplasmas, via an in vitro transcription assay.2016
Author(s)
Maejima, K., Miura, C., Koinuma, H., Iwabuchi, N., Nijo, T., Kitazawa, Y., Neriya, Y., Himeno, M., Oshima, K. and Namba, S.
Organizer
21st International Congress of the International Organization for Mycoplasmology
Place of Presentation
Brisbane, Australia
Year and Date
2016-07-03
Related Report
Int'l Joint Research
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[Presentation] ファイトプラズマの遺伝子発現制御のしくみをさぐる2016
Author(s)
前島健作, 三浦千裕, 岩渕望, 鯉沼宏章, 二條貴通, 北沢優悟, 煉谷裕太朗, 姫野未紗子, 大島研郎, 難波成任
Organizer
日本マイコプラズマ学会第43回学術集会
Place of Presentation
長崎県医師会館（長崎県長崎市）
Year and Date
2016-06-24
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