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Characterization of a novel leguminous symbiosis pathway derived by rhizobial effector

Research Project

Project/Area Number 16H04889
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

OKAZAKI SHIN  東京農工大学, (連合)農学研究科(研究院), 准教授 (40379285)

Co-Investigator(Kenkyū-buntansha) 林 誠  国立研究開発法人理化学研究所, 環境資源科学研究センター, チームリーダー (30291933)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2018: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2016: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Keywords共生 / 根粒菌 / マメ科植物 / 3型分泌系 / エフェクター / ダイズ / III型分泌系 / 土壌生物 / III型分泌機構
Outline of Final Research Achievements

Rhizobium-derived Nod factors (NFs) have been long believed to be essential for legume - rhizobium symbiosis. Bradyrhizobium elkanii USDA61, however, establishes symbiosis with soybean by a putative secreted protein Bel2-5 in the NF-independent manner. In this study, we expressed Bel2-5 protein in Escherichia coli and generated anti-Bel2-5 antibody and confirmed the secretion and localization of Bel2-5 in host cells. Besides,
when expressed in other rhizobia, Bel2-5 conferred the ability to modulate the NF receptor mutant soybean.
These results indicated that Bel2-5 is responsible for activating the NF-independent nodulation signaling.

Academic Significance and Societal Importance of the Research Achievements

マメ科植物と根粒菌の共生には根粒菌が作るNod factor (NF)が必須であると長い間考えられていた。本研究の成果は、この教科書的概念を覆し、根粒菌の分泌タンパク質(Bel2-5)が単独でマメ科植物の根粒形成シグナルを起動できることを示唆している。今後Bel2-5の宿主植物側標的や宿主細胞内での生化学的機能、NFシグナリングとの相違点を解明することで、マメ科植物と根粒菌の共進化を紐解く重要な知見となることが期待される。また、本エフェクターを利用することで、将来的にはイネやコムギなどNF受容機構のない非マメ科植物への根粒形成に貢献することが期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (23 results)

All 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (16 results) (of which Int'l Joint Research: 11 results,  Invited: 2 results) Remarks (2 results)

  • [Int'l Joint Research] ドレスデン工科大学(ドイツ)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Effects of Different Sources of Nitrogen on Endophytic Colonization of Rice Plants by <i>Azospirillum</i> sp. B5102018

    • Author(s)
      Naher K, Miwa H, Okazaki S, Yasuda M
    • Journal Title

      Microbes and Environments

      Volume: 33 Issue: 3 Pages: 301-308

    • DOI

      10.1264/jsme2.ME17186

    • NAID

      130007493355

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] InnB, a novel type III effector of Bradyrhizobium elkanii USDA61, controls symbiosis with Vigna Species2018

    • Author(s)
      Nguyen HP, Ratu STN, Yasuda M, Gottfert M, Okazaki S
    • Journal Title

      Frontiers in Microbiology

      Volume: 9 Pages: 1-10

    • DOI

      10.3389/fmicb.2018.03155

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] How effectors promote beneficial interactions2017

    • Author(s)
      Miwa Hiroki、Okazaki Shin
    • Journal Title

      Current Opinion in Plant Biology

      Volume: 38 Pages: 148-154

    • DOI

      10.1016/j.pbi.2017.05.011

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata2017

    • Author(s)
      Nguyen Hien、Miwa Hiroki、Kaneko Takakazu、Sato Shusei、Okazaki Shin
    • Journal Title

      Genes

      Volume: 8 Issue: 12 Pages: 374-374

    • DOI

      10.3390/genes8120374

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Infection mechanism of Bradyrhizobium elkanii by type III secretion system2018

    • Author(s)
      Michiko Yasuda、Shin Okazaki
    • Organizer
      JSME annual meeting & 10th ASME
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A novel T3 effector of Bradyrhizobium elkanii plays a dual function in symbiosis with legumes2018

    • Author(s)
      Hien P Nguyen、Safirah T. N. Ratu、Wint Y. T. Phoo、Michiko Yasuda、Shin Okazaki
    • Organizer
      JSME annual meeting & 10th ASME
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マメ科植物との共生における根粒菌 III型分泌系の機能解析2017

    • Author(s)
      岡崎 伸、三輪 大樹、Safirah Ratu、安田 美智子
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] A novel type III effector of Bradyrhizobium elkanii abolishing infection and nodule development in Vigna radiata2017

    • Author(s)
      Nguyen HP, Okazaki S
    • Organizer
      植物微生物研究会第27回研究交流会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Molecular mechanisms underlying Vigna radiata-rhizobia interactions2017

    • Author(s)
      Nguyen HP, Okazaki S
    • Organizer
      20th International Congress on Nitrogen Fixation
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Symbiotic roles of rhizobial type III effectors2017

    • Author(s)
      Okazaki S
    • Organizer
      20th International Congress on Nitrogen Fixation
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Bradyrhizobium elkanii の3型分泌系によるミヤコグサ根粒形成の制御2016

    • Author(s)
      Hiroki MIWA, Michiko YASUDA, Sachiko MASUDA, Takakazu KANEKO, Shusei SATO, Shin OKAZAKI
    • Organizer
      日本微生物生態学会第30回大会
    • Place of Presentation
      土浦亀城プラザ(茨城県土浦市)
    • Year and Date
      2016-10-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] Molecular genetic analysis of Bradyrhizobium elkanii mutants with altered symbiotic compatibility with Vigna radiata2016

    • Author(s)
      Hien Nguyen Phuoc, Faruque Omar, Hiroki Miwa, Shin Okazaki
    • Organizer
      日本微生物生態学会第30回大会
    • Place of Presentation
      土浦亀城プラザ(茨城県土浦市)
    • Year and Date
      2016-10-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] Characterization of transposon mutants of Bradyrhizobium elkanii USDA61 that formed effective nodule on Rj4 soybean cv. BARC22016

    • Author(s)
      Faruque Muhammad Omar, Michiko Yasuda, Hiroki Miwa, Yoshiharu Fujii , Takakazu Kaneko, Shusei Sato, Shin Okazaki
    • Organizer
      日本微生物生態学会第30回大会
    • Place of Presentation
      土浦亀城プラザ(茨城県土浦市)
    • Year and Date
      2016-10-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] Dual roles of rhizobial type III secretion system in soybean nodulation2016

    • Author(s)
      Shin Okazaki, Michiko Yasuda, Hiroki Miwa, Sachiko Masuda
    • Organizer
      4th Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
    • Place of Presentation
      マレーシア、ペナン
    • Year and Date
      2016-10-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Rhizobial type 3 effector proteins regulate nodulation of Lotus japonicus GIFU2016

    • Author(s)
      Hiroki Miwa, Michiko Yasuda, Sachiko Masuda, Takakazu Kaneko, Shusei Sato, Shin Okazaki
    • Organizer
      4th Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
    • Place of Presentation
      マレーシア、ペナン
    • Year and Date
      2016-10-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Bradyrhizobium elkanii genes involved in symbiotic incompatibility with Vigna radiate cv. KPS1 and Rj4 soybean2016

    • Author(s)
      Hien Nguyen Phuoc, Faruque M. Omar, Hiroki Miwa, Takakazu Kaneko, Shusei Sato, Shin Okazaki
    • Organizer
      4th Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
    • Place of Presentation
      マレーシア、ペナン
    • Year and Date
      2016-10-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Symbiotic roles of the type III secretion system in Bradyrhizobium elkanii2016

    • Author(s)
      Shin Okazaki, Hiroki Miwa, Michiko Yasuda, Sachiko Masuda
    • Organizer
      第12回ヨーロッパ窒素固定会議
    • Place of Presentation
      ハンガリー、ブタペスト
    • Year and Date
      2016-08-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Rhizobial type III effector protein regulates soybean nodulation2016

    • Author(s)
      Sachiko Masuda, Hiroki Miwa, Michiko Yasuda, Omar M. Faruqueand, Shin Okazaki
    • Organizer
      第12回ヨーロッパ窒素固定会議
    • Place of Presentation
      ハンガリー、ブタペスト
    • Year and Date
      2016-08-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effector-triggered Immunity Determines Host Genotype-specific Incompatibility in Legume-Rhizobium Symbiosis2016

    • Author(s)
      Michiko Yasuda, Hiroki Miwa, Sachiko Masuda, Yumiko Takebayashi, Hitoshi Sakakibara, Shin Okazaki
    • Organizer
      第12回ヨーロッパ窒素固定会議
    • Place of Presentation
      ハンガリー、ブタペスト
    • Year and Date
      2016-08-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Rhizobial type III secretion system and symbiosis with legumes2016

    • Author(s)
      Okazaki Shin
    • Organizer
      第17回国際植物微生物分子相互作用学会
    • Place of Presentation
      米国、オレゴン州ポートランド
    • Year and Date
      2016-07-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Remarks] 東京農工大学植物微生物学研究室

    • URL

      http://web.tuat.ac.jp/~okazaki/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 東京農工大学 農学府 国際環境農学専攻 植物微生物研究室 (岡崎研究室)

    • URL

      http://web.tuat.ac.jp/~okazaki/index.html

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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