| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Outline of Final Research Achievements |
Fundamental biological functions are typically consolidated during the stage after logarithmic growth phase and various phenomena related in this stage have been found to be associated with transcriptional machinery in Bacillus subtilis. Our findings are summarized in three aspects. One, the deceleration of transcription grant thermotolerance to the cells. Two, the altered preference of the initiation nucleotide confers stringent response independent on the cellular GTP level. Third, the altered preference of discriminator sequence makes the cells possible to recognize heterogeneous promoter. These findings uncovered new functions of RNA polymerase core enzyme, which had an important role for the survival strategy during the post-exponential growth phase. In addition, our comprehensive study on the stringent control revealed that an S-adenosyl methionine (SAM) had a role on the de novo GTP biosynthesis pathway by regulating the activity of the IMP dehydrogenase, GuaB.
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