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Regulation by intracellular regulators of plant ion transporters

Research Project

Project/Area Number 16H04906
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionTohoku University

Principal Investigator

Uozumi Nobuyuki  東北大学, 工学研究科, 教授 (40223515)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
Fiscal Year 2018: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
Keywordsイオンチャネル / シロイヌナズナ / 気孔閉鎖 / 脂質修飾 / リン酸化制御 / リン酸化酵素 / パルミトイル化 / カルシウム / 輸送体 / イオン / リン酸化 / 膜輸送体 / 気孔
Outline of Final Research Achievements

Ion transport system play an important role in control of the concentration of major ions in plant cells. They contribute to adjust intracellular osmotic pressure and maintain membrane potential by adapting to changes in environmental stress such as dryness, dehydration and salt damage. The transporter functions are involved in the opening and closing of pores responsible for carbon dioxide absorption and transpiration, environmental stress adaptation, and energy conversion of biological membranes. In order to maintain the homeostasis of cells to cope with environmental changes and to regulate the balance of nutrient absorption and excretion in the whole body of the plant, we elucidated the regulatory mechanism of anion channels mediating induction of stomatal closure. This regulation is performed by intracellular kinases, which indicate that intracellular molecules and transporters cooperate to adapt to environmental changes.

Academic Significance and Societal Importance of the Research Achievements

植物の細胞内の主要イオンの濃度を調節する陰イオン輸送体を制御する因子を明らかにした.これにより,乾燥・脱水・塩害などの環境ストレス変化に適応に関与する初期段階の分子レベルの調節系が明らかとなった.二酸化炭素の吸収や蒸散を担う気孔の開閉,環境ストレス適応,生体膜のエネルギー変換に関与することから,植物の養分吸収の改善,地球規模の環境変化に耐性を持つ植物の創出,環境保全,作物育種や環境変化に立ち向かう分子の創成につながる基盤的に知見を獲得した.

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (23 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (5 results) Journal Article (12 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 11 results,  Open Access: 4 results) Presentation (6 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results)

  • [Int'l Joint Research] University of Munster(ドイツ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University of Munster(ドイツ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of Padova(イタリア)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Stockholm University(Sweden)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Munster(Germany)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Guard Cell Membrane Anion Transport Systems and Their Regulatory Components: An Elaborate Mechanism Controlling Stress-Induced Stomatal Closure2019

    • Author(s)
      Saito Shunya、Uozumi Nobuyuki
    • Journal Title

      Plants

      Volume: 8 Issue: 1 Pages: 9-9

    • DOI

      10.3390/plants8010009

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] N-myristoylation and S-acylation are common modifications of Ca2+-regulated Arabidopsis kinases and are required for activation of the SLAC1 anion channel2018

    • Author(s)
      Saito Shunya、Hamamoto Shin、Moriya Koko、Matsuura Aiko、Sato Yoko、Muto Jun、Noguchi Hiroto、Yamauchi Seiji、Tozawa Yuzuru、Ueda Minoru、Hashimoto Kenji、Koester Philipp、Dong Qiuyan、Held Katrin、Kudla Joerg、Utsumi Toshihiko、Uozumi Nobuyuki
    • Journal Title

      New Phytologist

      Volume: 218 Issue: 4 Pages: 1504-1521

    • DOI

      10.1111/nph.15053

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] In vitro and in vivo characterization of modulation of the vacuolar cation channel TRPY1 from Saccharomyces cerevisiae2018

    • Author(s)
      Shin Hamamoto, Yasuo Mori, Isamu Yabe, Nobuyuki Uozumi
    • Journal Title

      The Febs Journal

      Volume: 285 Issue: 6 Pages: 1146-1161

    • DOI

      10.1111/febs.14399

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reduction of Spermidine Content Resulting from Inactivation of Two Arginine Decarboxylases Increases Biofilm Formation in Synechocystis sp. PCC 68032018

    • Author(s)
      Kota Kera, Tatsuya Nagayama, Kei Nanatani, Chika Saeki-Yamoto, Akira Tominaga, Satoshi Souma, Nozomi Miura, Kota Takeda, Syunsuke Kayamori, Eiji Ando, Kyohei Higashi, Kazuei Igarashi and Nobuyuki Uozumi
    • Journal Title

      Journal of Bacteriology

      Volume: 9 Issue: 9 Pages: 664-706

    • DOI

      10.1128/jb.00664-17

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Measurement of the mechanical properties of single Synechocystis sp. strain PCC6803 cells in different osmotic concentrations using a robot-integrated microfluidic chip2018

    • Author(s)
      Di Chang, Shinya Sakuma, Kota Kera, Nobuyuki Uozumi, Fumihito Arai
    • Journal Title

      Lab on a Chip

      Volume: 8 Issue: 8 Pages: 1241-1249

    • DOI

      10.1039/c7lc01245d

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ion Channels Regulate Nyctinastic Leaf Opening in Samanea saman2018

    • Author(s)
      Oikawa Takaya、Ishimaru Yasuhiro、Munemasa Shintaro、Takeuchi Yusuke、Washiyama Kento、Hamamoto Shin、Yoshikawa Nobuyuki、Mutara Yoshiyuki、Uozumi Nobuyuki、Ueda Minoru
    • Journal Title

      Current Biology

      Volume: 28 Issue: 14 Pages: 2230-2238

    • DOI

      10.1016/j.cub.2018.05.042

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cesium Inhibits Plant Growth Primarily Through Reduction of Potassium Influx and Accumulation in Arabidopsis2018

    • Author(s)
      Adams Eri、Miyazaki Takae、Saito Shunya、Uozumi Nobuyuki、Shin Ryoung
    • Journal Title

      Plant and Cell Physiology

      Volume: 60 Issue: 1 Pages: 63-76

    • DOI

      10.1093/pcp/pcy188

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and characterization of compounds that affect stomatal movements.2018

    • Author(s)
      Toh S, Inoue S, Toda Y, Yuki T, Suzuki K, Hamamoto S, Fukatsu K, Aoki S, Uchida M, Asai E, Uozumi N, Sato A, Kinoshita T.
    • Journal Title

      Plant & Cell Physiology

      Volume: in press Issue: 8 Pages: 1568-1580

    • DOI

      10.1093/pcp/pcy061

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Kup-mediated Cs+ uptake and Kdp-driven K+ uptake coordinate to promote cell growth during excess Cs+ conditions in Escherichia coli2017

    • Author(s)
      Ellen Tanudjaja, Naomi Hoshi, Yi-Hsin Su, Shin Hamamoto & Nobuyuki Uozumi
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 1-9

    • DOI

      10.1038/s41598-017-02164-7

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Probing native metal ion association sites through quenching of fluorophores in the nucleotide-binding domains of the ABC transporter MsbA2017

    • Author(s)
      Daiki Tatsumi, Kei Nanatani, Yuto Koike, Kiyoto Kamagata, Satoshi Takahashi, Ayumu Konno, Tadaomi Furuta, Minoru Sakurai, Nobuyuki Uozumi
    • Journal Title

      Biochem. J.

      Volume: 474 Issue: 12 Pages: 1993-2007

    • DOI

      10.1042/bcj20161051

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of regions responsible for the function of the plant K+ channels KAT1 and AKT2 in Saccharomyces cerevisiae and Xenopus laevis oocytes2017

    • Author(s)
      Saito Shunya、Hoshi Naomi、Zulkifli Lalu、Widyastuti Sri、Goshima Shinobu、Dreyer Ingo、Uozumi Nobuyuki
    • Journal Title

      Channels

      Volume: 11 Issue: 6 Pages: 510-516

    • DOI

      10.1080/19336950.2017.1372066

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The topogenic function of S4 promotes membrane insertion of the voltage-sensor domain in the KvAP channel2016

    • Author(s)
      Eriko Mishima, Yoko Sato, Kei Nanatani, Naomi Hoshi, Jong-Kook Lee, Nina Schiller, Gunnar von Heijne, Masao Sakaguchi, Nobuyuki Uozumi
    • Journal Title

      Biochem. J.

      Volume: 473 Issue: 23 Pages: 4361-4372

    • DOI

      10.1042/bcj20160746

    • Related Report
      2016 Annual Research Report
  • [Presentation] Ca2+キナーゼの膜局在化に依存した植物のイオンチャネル活性制御2018

    • Author(s)
      齋藤俊也,浜本晋,魚住信之
    • Organizer
      第13回トランスポーター研究会年会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Dual lipidation on Ca2+-dependent kinases governs SLAC1-mediated stomatal closure2018

    • Author(s)
      Shunya Saito, Shin Hamamoto, Nobuyuki Uozumi
    • Organizer
      International Symposium on Photosynthesis and Chloroplast Biogenesis 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] CPK6キナーゼの膜局在化に依存した気孔閉鎖誘導チャネルSLAC1の調節2017

    • Author(s)
      齋藤俊也, 浜本晋, 内海俊彦, 守屋康子, 佐藤陽子, 野口寛人, 戸澤譲, 山内清司, 橋本研志, Joerg Kudla, 魚住信之
    • Organizer
      第12回トランスポーター研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Regulation of plant Cl channels and role of bacterial K/Cs transporters2017

    • Author(s)
      Nobuyuki Uozumi
    • Organizer
      CSRSセミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 生体金属の吸収,排出,循環を司る微生物と植物の膜タンパク質2017

    • Author(s)
      魚住信之
    • Organizer
      生体金属動態研究会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Lipidation of Arabidopsis CPK6 promotes the plasma membrane targeting and the stomata closure mediated by activation of SLAC1 in Arabidopsis guard cells2016

    • Author(s)
      Saito, S., Hamamoto, S., Sato, Y., Uozumi, N., Hashimoto, K., Kudla, J., Utsumi, T., Moriya, K., Tozawa, Y., and Yamauchi, S.
    • Organizer
      International workshop on Plant Membrane Biology
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2020-03-30  

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