| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Outline of Final Research Achievements |
The goal of this study is to clarify the metabolism and physiological functions of D-amino acids, and their application to various research tools. The following studies were conducted. 1) Serine racemase, which is responsible for the biosynthesis of D-Ser, a co-agonist of NMDA receptor, was confirmed to catalyze the D-serine dehydration. 2) An unknown D-amino acid was found in an edible mushroom, Hypsizygus marmoreus, and identified as D-2-amino-3,4-dihydroxy-butanoic acid. (3) Urinary D-Ser concentrations of renal disease patients and healthy subjects were analyzed. Ratio of the urinary D-Ser concentration to that of urinary creatinine was suggested to serve as a biomarker of renal disorder. (4) Functional cloning system of the genes encoding D-amino acid synthesizing enzymes was constructed with E. coli mutant lacking alanine racemase and glutamate racemase genes.
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