Studies of bio-regulation with D-amino acids and its application
| Project/Area Number |
16H04908
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Keywords | D-アミノ酸 / D-セリン / セリンラセマーゼ / D-セリンデヒドラターゼ / 腎疾患マーカー / D-アスパラギン酸 / D-アミノ酸トランスアミナーゼ / 酵素 / D－アミノ酸 / バイオテクノロジー / 腎障害マーカー / 細胞性粘菌 / 生理活性 / 分析 |
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| Outline of Final Research Achievements |
The goal of this study is to clarify the metabolism and physiological functions of D-amino acids, and their application to various research tools. The following studies were conducted. 1) Serine racemase, which is responsible for the biosynthesis of D-Ser, a co-agonist of NMDA receptor, was confirmed to catalyze the D-serine dehydration. 2) An unknown D-amino acid was found in an edible mushroom, Hypsizygus marmoreus, and identified as D-2-amino-3,4-dihydroxy-butanoic acid. (3) Urinary D-Ser concentrations of renal disease patients and healthy subjects were analyzed. Ratio of the urinary D-Ser concentration to that of urinary creatinine was suggested to serve as a biomarker of renal disorder. (4) Functional cloning system of the genes encoding D-amino acid synthesizing enzymes was constructed with E. coli mutant lacking alanine racemase and glutamate racemase genes.
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| Academic Significance and Societal Importance of the Research Achievements |
D-アミノ酸の生理機能が注目されている。例えば D-Serは記憶や学習などの脳機能に、D-Aspは脳ホルモンの分泌などに関与する。本研究では D-アミノ酸の機能や代謝の解明、様々な研究ツールへの応用を試みた。具体的には、D-Ser合成を担うセリンラセマーゼがD-Serの分解にも働くこと、ブナシメジに新奇D-アミノ酸、D-2-amino-3,4-dihydroxy-butanoic acidが存在すること、尿中のD-Serとクレアチニンの濃度比が腎疾患マーカーへ応用可能であることを示した。また D-アミノ酸研究の強力なツールとなるD-アミノ酸生合成酵素遺伝子スクリーニング系を構築した。
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Occurrence of the (2R,3S)-Isomer of 2-Amino-3,4-dihydroxybutanoic Acid in the Mushroom Hypsizygus marmoreus.2017
Author(s)
Ito T, Yu Z, Yoshino I, Hirozawa Y, Yamamoto K, Shinoda K, Watanabe A, Hemmi Ｈ, Asada Y, Yoshimura T.
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Journal Title
J Agric Food Chem.
Volume: 65
Issue: 30
Pages: 6131-6139
DOI
Related Report
Peer Reviewed
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