Reactions specific to active selenium species in selenoprotein biosynthesis
Project/Area Number |
16H04913
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied biochemistry
|
Research Institution | Ritsumeikan University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
戸部 隆太 立命館大学, 生命科学部, 助教 (00758823)
|
Project Period (FY) |
2016-04-01 – 2019-03-31
|
Project Status |
Completed (Fiscal Year 2018)
|
Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2018: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
|
Keywords | セレン / セレンタンパク質 / セレノシステイン / チオレドキシン / 亜セレン酸還元酵素 / 活性型セレン / 微量元素 / セレノプロテイン |
Outline of Final Research Achievements |
We identified the chemical structure of active selenium that was formed on the active site of thioredoxin as an intermediate in the process prior to the supply of selenium substrate to selenophosphate synthetase, which is required for selenoprotein biosynthesis. We also found a novel selenite reductase that has a selenocysteine residue and five c-type heme in a dissimilatory metal-reducing bacterium Geobacter sulfurreducens. The enzymatic properties of the selenoprotein was investigated. In addition, we showed that a gene encoding the novel selenite reductase is involved in the formation of elemental selenium on the surface of the bacterial cells.
|
Academic Significance and Societal Importance of the Research Achievements |
必須微量元素の作用は、微量であるがゆえに解析が難しく未開拓な部分が多い。セレンの研究は、他の微量元素(鉄、銅、亜鉛など)に比べると、栄養学的な面と細胞、分子、原子のレベルとの橋渡しが未だ十分になされていない。本研究により、微量元素の体内動態を特徴づける一般則およびセレン代謝制御の特殊性の双方の一端が明らかになったと考えられる。また、セレンの栄養学、病理学の発展への波及効果が期待される。
|
Report
(4 results)
Research Products
(26 results)
-
-
-
-
-
-
-
-
[Journal Article] A non-radioactive assay for selenophosphate synthetase activity using recombinant pyruvate pyrophosphate dikinase from Thermus thermophilus HB82016
Author(s)
Kamada, S., Okugochi, T., Asano, K., Tobe, R., Mihara, H., Nemoto, M., Inagaki, K., and Tamura, T.
-
Journal Title
Biosci. Biotechnol. Biochem.
Volume: 80
Issue: 10
Pages: 1970-1972
DOI
Related Report
Peer Reviewed
-
-
-
[Presentation] Selenium metabolism in Geobacter sulfurreducens2018
Author(s)
Mihara, H., Jinno, M., Shimamoto, N., Jahan, M. I., Yamane, Y., Kawakami, M., Ishido, Y., and Tobe, R.
Organizer
The 7th International Selenium Conference (Se2018)
Related Report
Int'l Joint Research
-
[Presentation] Selenite reduction in the dissimilatory metal-reducing bacterium Geobacter sulfurreducens2018
Author(s)
Mihara, H., Jahan, M. I., Jinno, M., Shimamoto, N., Matsuzaki, Y., Izu, Y., and Tobe, R
Organizer
12th International Congress of Extremophiles (Extremophiles 2018)
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
[Presentation] A novel operon involved in selenite reduction in Geobacter sulfurreducens2017
Author(s)
Mihara, H., Jinno, M., Shimamoto, N., Matsuzaki, Y., Jahan, M. I., Yamane, Y., Tobe, R., and Kadiri, O. H.
Organizer
Se2017 (The 11th International Symposium on Selenium in Biology and Medicine and The 5th International Conference on Selenium in the Environment and Human Health)
Related Report
Int'l Joint Research
-
[Presentation] Selenium delivery system for selenophosphate synthetase in bacteria2016
Author(s)
Tobe, R., Shimizu, A., Hagita, S., Prakash, N. T., and Mihara, H.
Organizer
The 6th International Selenium Conference (2016)
Place of Presentation
Shenzhen, China
Year and Date
2016-10-22
Related Report
Int'l Joint Research
-
-
-
-
-
-