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Physiological function of the receptor expressing intestinal tuft cells and screening of its inhibitors

Research Project

Project/Area Number 16H04919
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Food science
Research InstitutionMeiji University

Principal Investigator

Ishimaru Yoshiro  明治大学, 農学部, 専任准教授 (10451840)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2016: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Keywords受容体 / 刷子細胞 / 代謝 / 免疫 / 遺伝子破壊マウス / 消化管
Outline of Final Research Achievements

To elucidate the function of the orphan receptor Gprc5c, which is expressed in intestinal tuft cells, in vivo, we generated its knockout mice by new genome editing methods, TALEN and CRISPER/Cas9 and analyzed its phenotypes. KO mice have reduced body weight. Infection experiments showed that Gprc5c may not be involved in type 2 innate immune response against nematode, Nippostrongylus brasiliensis, infection.

Academic Significance and Societal Importance of the Research Achievements

近年、アレルギー性疾患やメタボリックシンドロームは解決すべき重要な社会問題になっている。小腸刷子細胞の頂端部に局在するオーファン受容体の生理機能が解明され、この受容体を阻害する食品由来因子などの化合物を見つけることができれば、アレルギー性疾患や肥満・糖尿病の予防や治療に繋げることが期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (24 results)

All 2019 2018 2017 2016 Other

All Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 9 results,  Open Access: 7 results,  Acknowledgement Compliant: 3 results) Presentation (9 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Book (5 results) Remarks (1 results)

  • [Journal Article] Positive/Negative Allosteric Modulation Switching in an Umami Taste Receptor (T1R1/T1R3) by a Natural Flavor Compound, Methional.2018

    • Author(s)
      Toda Y, Nakagita T, Hirokawa T, Yamashita Y, Nakajima A, Narukawa M, Ishimaru Y, Uchida R, Misaka T.
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 11796-11796

    • DOI

      10.1038/s41598-018-30315-x

    • NAID

      120006546000

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Gene Expression Analysis of the Effect of Ischemic Infarction in Whole Blood2017

    • Author(s)
      Takuma Ayako、Abe Arata、Saito Yoshikazu、Nito Chikako、Ueda Masayuki、Ishimaru Yoshiro、Harada Hideki、Abe Keiko、Kimura Kazumi、Asakura Tomiko
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 18 Issue: 11 Pages: 2335-2335

    • DOI

      10.3390/ijms18112335

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A large increase of sour taste receptor cells in Skn-1-deficient mice does not alter the number of their sour taste signal-transmitting gustatory neurons2017

    • Author(s)
      Maeda N, Narukawa M, Ishimaru Y, Yamamoto K, Misaka T and Abe K
    • Journal Title

      Neuroscience Letters

      Volume: 印刷中 Pages: 53-58

    • DOI

      10.1016/j.neulet.2017.03.046

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Expanding frontiers in weight-control research explored by young investigators.2017

    • Author(s)
      Ishimaru Y, Kozuka C, Nakajima K, and Sasaki T
    • Journal Title

      J. Physiol. Sci.

      Volume: 67 Issue: 1 Pages: 83-95

    • DOI

      10.1007/s12576-016-0495-7

    • NAID

      40021064558

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Olfactory receptor accessory proteins play crucial roles in receptor function and gene choice2017

    • Author(s)
      R. Sharma, Y. Ishimaru, I. Davison, K. Ikegami, M. S. Chien, H. You, Q. Chi, M. Kubota, M. Yohda, M. Ehlers, H. Matsunami
    • Journal Title

      Elife

      Volume: 印刷中

    • DOI

      10.7554/elife.21895

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Catecholamines facilitate fuel expenditure and protect against obesity via a novel network of the gut-brain axis in transcription factor Skn-1-deficient mice.2016

    • Author(s)
      Ushiama, S., Ishimaru, Y., Narukawa, M., Yoshioka, M., Kozuka, C., Watanabe, N., Tsunoda, M., Osakabe, N., Asakura, T., Masuzaki, H., and Abe, K.
    • Journal Title

      EBioMedicine

      Volume: 8 Pages: 60-71

    • DOI

      10.1016/j.ebiom.2016.04.031

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Variation in ligand responses of the bitter taste receptors TAS2R1 and TAS2R4 among New World monkeys.2016

    • Author(s)
      K. Tsutsui, M. Otoh, K. Sakurai, N. Suzuki-Hashido, T. Hayakawa, T. Misaka, Y. Ishimaru, F. Aureli, A. D. Melin, *S. Kawamura, *H. Imai.
    • Journal Title

      BMC Evolutionary Biology

      Volume: 16 Issue: 1 Pages: 208-208

    • DOI

      10.1186/s12862-016-0783-0

    • NAID

      120006223634

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Spontaneous Bilateral Cervical Internal Carotid and Vertebral Artery Dissection in a Japanese Patient without Collagen Vascular Disease with Special Reference to Single-Nucleotide Polymorphisms2016

    • Author(s)
      Abe Arata、Nito Chikako、Sakamoto Yuki、Nogami Akane、Hokama Hiroyuki、Takahashi Shiro、Kirita Kumiko、Ueda Masayuki、Ishimaru Yoshiro、Kimura Kazumi
    • Journal Title

      Journal of Stroke and Cerebrovascular Diseases

      Volume: 25 Issue: 8 Pages: e114-e117

    • DOI

      10.1016/j.jstrokecerebrovasdis.2016.04.027

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Amino acid residues of bitter taste receptor TAS2R16 that determine sensitivity in primates to β-glycosides2016

    • Author(s)
      Imai Hiroo, Suzuki-Hashido Nami, Ishimaru Yoshiro, Takanobu Sakurai, Lijie Yin, Wenshi Pan, Masaji Ishiguro, Masuda Katsuyoshi, Abe Keiko, Misaka Takumi, Hirai Hirohisa
    • Journal Title

      Biophysics and Physicobiology

      Volume: 13 Issue: 0 Pages: 165-171

    • DOI

      10.2142/biophysico.13.0_165

    • NAID

      130005165371

    • ISSN
      2189-4779
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] コイ味覚受容体の発現解析2019

    • Author(s)
      久保園 峻、戸田 安香、石丸 喜朗
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 霊長類T1R1/T1R3におけるグルタミン酸受容能獲得の分子基盤:クモザルに注目した検討2019

    • Author(s)
      戸田 安香、早川 卓志、中北 智哉、河村 正二、今井 啓雄、石丸 喜朗、三坂 巧
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 小腸刷子細胞頂端部に局在する受容体の生体内機能の検証2018

    • Author(s)
      石丸 喜朗
    • Organizer
      日本味と匂学会第52回大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 脊椎動物における味覚受容体T1Rファミリーの進化解析2018

    • Author(s)
      西原 秀典、戸田 安香、工楽 樹洋、蔵本 多恵、岡部 正隆、石丸 喜朗
    • Organizer
      日本進化学会第20回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 小腸刷子細胞頂端部に局在する受容体の生体内機能の検証2017

    • Author(s)
      谷下道大, 成川真隆, 戸田安香, 石渡賢治, 吉岡美紗子, 朝倉富子, 嘉糠洋陸, 益崎裕章, 阿部啓子, 石丸喜朗
    • Organizer
      第71回日本栄養・食糧学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 霊長類における旨味受容体の機能解析2017

    • Author(s)
      戸田安香, 石丸喜朗
    • Organizer
      日本味と匂学会第51回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Intestinal tuft cells: immunity and metabolism2017

    • Author(s)
      Yoshiro Ishimaru
    • Organizer
      The 16th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 小腸刷子細胞に発現する遺伝子の生体内機能の検証2017

    • Author(s)
      谷下 道大、成川 真隆、石渡 賢治、吉岡 美紗子、朝倉 富子、嘉糠 洋陸、阿部 啓子、石丸 喜朗
    • Organizer
      日本農芸化学会2017年度京都大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Catecholamines Facilitate Fuel Expenditure and Protect Against Obesity via a Novel Network of the Gut-Brain Axis in Transcription Factor Skn-1 -deficient Mice2016

    • Author(s)
      Ishimaru Yoshiro、Ushiama Shota、Narukawa Masataka、Yoshioka Misako、Kozuka Chisayo、Watanabe Naoki、Tsunoda Makoto、Osakabe Naomi、Asakura Tomiko、Masuzaki Hiroaki、Abe Keiko
    • Organizer
      ISOT2016
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Book] アレルギー性疾患や寄生虫感染と刷子細胞2019

    • Author(s)
      石丸 喜朗
    • Total Pages
      5
    • Publisher
      医歯薬出版
    • Related Report
      2018 Annual Research Report
  • [Book] 味覚と味覚受容体2018

    • Author(s)
      石丸 喜朗
    • Total Pages
      5
    • Publisher
      医歯薬出版
    • Related Report
      2018 Annual Research Report
  • [Book] さまざまな脊椎動物における味覚の進化2018

    • Author(s)
      戸田 安香、石丸 喜朗
    • Total Pages
      5
    • Publisher
      医歯薬出版
    • Related Report
      2018 Annual Research Report
  • [Book] アグリバイオ 2017年8月臨時増刊号2017

    • Author(s)
      阿部 啓子・中嶋 康博
    • Total Pages
      100
    • Publisher
      北隆館
    • Related Report
      2017 Annual Research Report
  • [Book] おいしさの科学的評価・測定法と応用展開2016

    • Author(s)
      阿部啓子、石丸喜朗
    • Total Pages
      212
    • Publisher
      シーエムシー出版
    • ISBN
      9784781311777
    • Related Report
      2016 Annual Research Report
  • [Remarks] 腸脳軸を介した新しいエネルギー代謝調節機構を解明

    • URL

      http://www.a.u-tokyo.ac.jp/topics/2016/20160531-1.html

    • Related Report
      2016 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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