Physiological function of the receptor expressing intestinal tuft cells and screening of its inhibitors
Project/Area Number |
16H04919
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Food science
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Research Institution | Meiji University |
Principal Investigator |
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Project Period (FY) |
2016-04-01 – 2019-03-31
|
Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2016: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
|
Keywords | 受容体 / 刷子細胞 / 代謝 / 免疫 / 遺伝子破壊マウス / 消化管 |
Outline of Final Research Achievements |
To elucidate the function of the orphan receptor Gprc5c, which is expressed in intestinal tuft cells, in vivo, we generated its knockout mice by new genome editing methods, TALEN and CRISPER/Cas9 and analyzed its phenotypes. KO mice have reduced body weight. Infection experiments showed that Gprc5c may not be involved in type 2 innate immune response against nematode, Nippostrongylus brasiliensis, infection.
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Academic Significance and Societal Importance of the Research Achievements |
近年、アレルギー性疾患やメタボリックシンドロームは解決すべき重要な社会問題になっている。小腸刷子細胞の頂端部に局在するオーファン受容体の生理機能が解明され、この受容体を阻害する食品由来因子などの化合物を見つけることができれば、アレルギー性疾患や肥満・糖尿病の予防や治療に繋げることが期待される。
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Olfactory receptor accessory proteins play crucial roles in receptor function and gene choice2017
Author(s)
R. Sharma, Y. Ishimaru, I. Davison, K. Ikegami, M. S. Chien, H. You, Q. Chi, M. Kubota, M. Yohda, M. Ehlers, H. Matsunami
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Journal Title
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Catecholamines facilitate fuel expenditure and protect against obesity via a novel network of the gut-brain axis in transcription factor Skn-1-deficient mice.2016
Author(s)
Ushiama, S., Ishimaru, Y., Narukawa, M., Yoshioka, M., Kozuka, C., Watanabe, N., Tsunoda, M., Osakabe, N., Asakura, T., Masuzaki, H., and Abe, K.
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Journal Title
EBioMedicine
Volume: 8
Pages: 60-71
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Variation in ligand responses of the bitter taste receptors TAS2R1 and TAS2R4 among New World monkeys.2016
Author(s)
K. Tsutsui, M. Otoh, K. Sakurai, N. Suzuki-Hashido, T. Hayakawa, T. Misaka, Y. Ishimaru, F. Aureli, A. D. Melin, *S. Kawamura, *H. Imai.
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Journal Title
BMC Evolutionary Biology
Volume: 16
Issue: 1
Pages: 208-208
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Amino acid residues of bitter taste receptor TAS2R16 that determine sensitivity in primates to β-glycosides2016
Author(s)
Imai Hiroo, Suzuki-Hashido Nami, Ishimaru Yoshiro, Takanobu Sakurai, Lijie Yin, Wenshi Pan, Masaji Ishiguro, Masuda Katsuyoshi, Abe Keiko, Misaka Takumi, Hirai Hirohisa
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Journal Title
Biophysics and Physicobiology
Volume: 13
Issue: 0
Pages: 165-171
DOI
NAID
ISSN
2189-4779
Related Report
Peer Reviewed
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