Enhanced mucin secretory by dietary fibers and its application to maintain gut barrier functions

• Project/Area Number: 16H04920
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Shizuoka University
• Principal Investigator: Morita Tatsuya 静岡大学, 農学部, 教授 (90332692)
• Co-Investigator(Kenkyū-buntansha): 日野 真吾 静岡大学, 農学部, 准教授 (70547025)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2018: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
• Keywords: 食物繊維 / ムチン / 栄養学 / 食品

Outline of Final Research Achievements

The purpose of this study was to clarify the effects of enhanced mucin secretion by dietary fiber intake on intestinal homeostasis, especially the barrier function. The following results were obtained. (1) The intestinal permeability in rats fed indigestible oligosaccharides revealed that short term ingestion of indigestible oligosaccharides reduced gut permeability and destroyed the cecal mucin layer. But the changes was canceled by long-term ingestion. These temporal changes in rats fed indigestible oligosaccharides seems to be related to the fermentation pattern by intestinal bacteria. (2) We found that mucin secreted in the digestive tract may promote fecal excretion by functioning as a lubricant in rats fed phyllium. (3) Pectin hydrolyzates were newly prepared as a material to promote mucin secretion in the small intestine. In vitro studies revealed that pectin hydrolyzates have 10-100 times greater mucin secretory effects compared to pectin.

Academic Significance and Societal Importance of the Research Achievements

近年，代謝疾患を含む様々な疾患の発症およびその促進因子として消化管バリア機能の低下や腸内細菌叢との関連が挙げられている。本研究では，食物繊維摂取によって変動するムチンが消化管でどのような影響を及ぼすのかについて多面的に解析を行うことで公衆栄養的な食物繊維摂取の意義について再考するとともに，消化管ムチンの機能性について明らかにしようとした。食物繊維の摂取は，それ自身が腸内細菌の発酵基質となり腸管機能に影響を与えることに加え，ムチン分泌を修飾することで間接的にも影響を与える可能性を示した点に学術的意義があると考えている。

Research Products

• [Journal Article] The Impact of Fructo-Oligosaccharides on Gut Permeability and Inflammatory Responses in the Cecal Mucosa Quite Differs between Rats Fed Semi-Purified and Non-Purified Diets (2018)
  • Author(s): GENDA Tomomi、KONDO Takashi、HINO Shingo、SUGIURA Shunsaku、NISHIMURA Naomichi、MORITA Tatsuya
  • Journal Title: Journal of Nutritional Science and Vitaminology Volume: 64 Issue: 5 Pages: 357-366
  • DOI: 10.3177/jnsv.64.357
  • NAID: 130007501996
  • ISSN: 0301-4800, 1881-7742
  • Year and Date: 2018-10-31
  • Peer Reviewed
• [Journal Article] Bacterial Fermentation of Water-Soluble Cellulose Acetate Raises Large-Bowel Acetate and Propionate and Decreases Plasma Cholesterol Concentrations in Rats (2018)
  • Author(s): Genda Tomomi、Kondo Takashi、Sugiura Shunsaku、Hino Shingo、Shimamoto Shu、Nakamura Toshikazu、Ukita Shizuka、Morita Tatsuya
  • Journal Title: Journal of Agricultural and Food Chemistry Volume: 66 Issue: 45 Pages: 11909-11916
  • DOI: 10.1021/acs.jafc.8b04093
• [Journal Article] Fructo-oligosaccharide-Induced Transient Increases in Cecal Immunoglobulin A Concentrations in Rats Are Associated with Mucosal Inflammation in Response to Increased Gut Permeability (2017)
  • Author(s): Genda Tomomi、Sasaki Yuta、Kondo Takashi、Hino Shingo、Nishimura Naomichi、Tsukahara Takamitsu、Sonoyama Kei、Morita Tatsuya
  • Journal Title: The Journal of Nutrition Volume: 147 Issue: 10 Pages: 1900-1908
  • DOI: 10.3945/jn.117.253955
  • Peer Reviewed
• [Journal Article] Digestion-Resistant Dextrin Derivatives Are Moderately Digested in the Small Intestine and Contribute More to Energy Production Than Predicted from Large-Bowel Fermentation in Rats (2017)
  • Author(s): Kondo Takashi、Handa Kei、Genda Tomomi、Hino Shingo、Hamaguchi Norihisa、Morita Tatsuya
  • Journal Title: The Journal of Nutrition Volume: 147 Issue: 3 Pages: 330-336
  • DOI: 10.3945/jn.116.239855
  • Peer Reviewed
• [Journal Article] Fructans in the first 1000 days of life and beyond, and for pregnancy. (2016)
  • Author(s): Firmansyah A, Chongviriyaphan N, Dillon DH, Khan NC, Morita T, Tontisirin K, Tuyen LD, Wang W, Bindels J, Deurenberg P, Ong S, Hautvast J, Meyer D, Vaughan EE.
  • Journal Title: Asia Pac J Clin Nutr. Volume: 25(4) Pages: 652-675
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] High wholegrain barley β-glucan lowers food intake but does not alter small intestinal macronutrient digestibility in ileorectostomised rats. (2016)
  • Author(s): Belobrajdic DP, Hino S, Kondo T, Jobling SA, Morell MK, Topping DL, Morita T, Bird AR.
  • Journal Title: Int J Food Sci Nutr. Volume: 67(6) Pages: 678-85
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] イヌリン型フルクタンの免疫応答と大腸生理 (2016)
  • Author(s): 源田知美,森田達也
  • Journal Title: 日本応用糖質科学会誌 Volume: 6(4) Pages: 212-218
• [Presentation] ムチン型糖タンパク質の摂取が大腸短鎖脂肪酸量および小腸ムチン糖鎖に及ぼす影響の解析 (2018)
  • Author(s): 石川愛梨,宮田高明, 日野真吾, 森田達也
  • Organizer: 日本栄養・食糧学会 中部支部大会