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Tracking the route of atmospheric nitrogen to diazotrophs colonizing buried mangrove roots

Research Project

Project/Area Number 16H04941
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Forest science
Research InstitutionNational Institute for Environmental Studies

Principal Investigator

Inoue Tomomi  国立研究開発法人国立環境研究所, 生物・生態系環境研究センター, 主任研究員 (80435578)

Co-Investigator(Kenkyū-buntansha) 下野 綾子  東邦大学, 理学部, 講師 (30401194)
高津 文人  国立研究開発法人国立環境研究所, 地域環境研究センター, 室長 (30514327)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2019: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Keywordsマングローブ / 地上根 / 窒素固定 / 安定同位体窒素 / メタゲノム / 通気組織 / 呼吸根 / マングローブ植物 / 通気システム
Outline of Final Research Achievements

Nitrogen-fixing activity has been observed in the rhizosphere of mangrove ecosystems, suggesting a close mangrove–diazotroph relationship. In regularly flooded soil, however, the pathway by which atmospheric nitrogen reaches the diazotrophs in the rhizosphere is unknown. This study provides evidence that mangrove aerial roots serve as pathways that supply nitrogen gas to the diazotrophs colonizing buried roots. A plastic chamber was attached on the exposed part of a Rhizophora stylosa prop root and 15N2 tracer gas was injected into it. The entire root, including the below-ground part, was collected for analysis of 15N labelling and nitrogenase activity. We detected 15N labelling in buried root materials 2 h after gas injection. Compared with the δ15N contents in root material from an untreated tree, the increment was >10‰ in lateral roots.

Academic Significance and Societal Importance of the Research Achievements

マングローブ植物に特徴的にみられる地上根は、多くの植物学者の興味の対象となってきた。地上根内部には、地下に繋がる空隙(通気組織)が発達しているため、冠水を繰り返す干潟環境に対応した酸素供給経路であると考えれてきている。本研究結果は、ヤエヤマヒルギの地上根通気組織内を大気ガスが移動していることを示した初めての報告である。また、この経路が酸素だけではなく窒素の供給経路にもなっていることが明らかとなり、マングローブ植物の窒素獲得機構に関する新しい知見を得ることが出来た。
この知見は、気候変動緩和の観点において近年注目を浴びているマングローブ生態系の炭素貯留機能の理解にも貢献する。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (33 results)

All 2020 2019 2018 2017 2016

All Journal Article (15 results) (of which Int'l Joint Research: 9 results,  Peer Reviewed: 15 results,  Open Access: 4 results) Presentation (16 results) (of which Int'l Joint Research: 7 results,  Invited: 5 results) Book (2 results)

  • [Journal Article] Phenolic constituents and anticancer properties of Morus alba (white mulberry) leaves2020

    • Author(s)
      Chan E. W. C., Wong S.K., Tangah J., Inoue T., Chan H.T.
    • Journal Title

      Journal of Integrative Medicine

      Volume: 18 Pages: 189-195

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tracking the route of atmospheric nitrogen to diazotrophs colonizing buried mangrove roots2019

    • Author(s)
      Inoue Tomomi、Kohzu Ayato、Shimono Ayako
    • Journal Title

      Tree Physiology

      Volume: 39 Issue: 11 Pages: 1896-1906

    • DOI

      10.1093/treephys/tpz088

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mangrove - diazotroph relationships at the root, tree and forest scales: diazotrophic communities create high soil nitrogenase activities in Rhizophora stylosa rhizospheres2019

    • Author(s)
      Inoue Tomomi、Shimono Ayako、Akaji Yasuaki、Baba Shigeyuki、Takenaka Akio、Tuck Chan Hung
    • Journal Title

      Annals of Botany

      Volume: 125 Pages: 131-144

    • DOI

      10.1093/aob/mcz164

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adaptation to climate change through mangrove rehabilitation involving local community participation2019

    • Author(s)
      Baba, S., Chan, H.T., Kainuma, M., Oshiro, N., Kezuka, M., Kimura, N., Inoue, T.
    • Journal Title

      ISME/GLOMIS Electronic Journal

      Volume: 17 Pages: 4-14

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Holocene sea-level change and evolution of a mixed coral reef and mangrove system at Iriomote Island, southwest Japan2019

    • Author(s)
      Yamano H., Inoue T., Adachi H., Tsukaya K., Adachi R., Baba S.
    • Journal Title

      Estuarine, Coastal and Shelf Science

      Volume: 220 Pages: 166-175

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photosynthesis, respiration, and growth patterns of Rhizophora stylosa seedlings in relation to growth temperature2019

    • Author(s)
      Akaji Y, Inoue T, Tomimatsu H, Kawanishi A
    • Journal Title

      Trees

      Volume: 未定 Issue: 4 Pages: 1041-1049

    • DOI

      10.1007/s00468-019-01840-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phenolic constituents from the root bark of Morus alba with emphasis on morusin and its anti-cancer properties2019

    • Author(s)
      Chan E. W. C., Wong S.K., Inoue T., Chan H.T.
    • Journal Title

      Journal of Chinese Pharmaceutical Sciences

      Volume: 28 Pages: 75-87

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Caesalpinia crista: A coastal woody climber with promising therapeutic values2018

    • Author(s)
      Chan E. W. C., Tangah J., Baba S., Chan H. T., Kainuma M., Inoue T.
    • Journal Title

      Journal of Applied Pharmaceutical Science

      Volume: 8 Pages: 133-140

    • DOI

      10.7324/japs.2018.8319

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Botany, Uses, Chemistry and Pharmacology of Ficus microcarpa: A Short Review2017

    • Author(s)
      Chan E.W.C., Tangah J., Inoue T., Kainuma M., Baba K., Oshiro N., Kezuka M., Kimura N.
    • Journal Title

      Systematic Reviews in Pharmacy

      Volume: 8 Issue: 1 Pages: 103-111

    • DOI

      10.5530/srp.2017.1.18

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Botany, uses, chemistry and bioactivities of mangrove plants V: Acrostichum aureum and A. speciosum.2017

    • Author(s)
      Kimura N., Kainuma M., Inoue T., Chan E.W.C., Tangah J., Baba K., Oshiro N., Okamoto C.
    • Journal Title

      ISME/GLOMIS

      Volume: 15 Pages: 1-6

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Garcinia subelliptica (Fukugi): A Multi-purpose Coastal Tree with Promising Medicinal Properties2017

    • Author(s)
      Inoue T., Kainuma M., Baba K., Oshiro N., Kimura N., Chan E. W. C.
    • Journal Title

      Journal of Intercultural Ethnopharmacology

      Volume: 6 Pages: 121-127

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Ulam herbs: A review on the medicinal properties of Anacardium occidentale and Barringtonia racemose2017

    • Author(s)
      Chan E. W. C., Baba S., Chan H. T., Kainuma M., Inoue T., Wong S. K
    • Journal Title

      Journal of Applied Pharmaceutical Science

      Volume: 7 Pages: 241-247

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Real-time in-situ Simultaneous Monitoring of Dissolved Oxygen and Materials Movements at a Vicinity of Micrometers from an Aquatic Plant by Combining Deflection of a Probe Beam and Fluorescence Quenching2017

    • Author(s)
      WU X Z., WU X., Inoue T.
    • Journal Title

      Analytical Sciences

      Volume: 33 Pages: 351-355

    • NAID

      130005439482

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Botany, uses, chemistry and bioactivities of mangrove plants IV: Avicennia marina.2016

    • Author(s)
      Baba S., Chan H.T., Oshiro N., Maxwell G. S., Inoue T., Chan E. W. C.
    • Journal Title

      ISME/GLOMIS

      Volume: 14 Pages: 5-10

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Artocarpus altilis and Pandanus tectorius: Two important fruits of Oceania with medicinal values.2016

    • Author(s)
      Baba S., Chan H.T., Kezuka M., Inoue T., Chan E.W.C.
    • Journal Title

      Journal of Food and Agriculture

      Volume: 28 Pages: 531-539

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] マングローブ植物の葉と根の呼吸温度依存特性2020

    • Author(s)
      井上智美、野口航、赤路康朗
    • Organizer
      日本生態学会大会第67回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Rhizophore diazotrophic communities developing with mangrove forest formation2020

    • Author(s)
      Shimono A., Inoue T.
    • Organizer
      The 67th Annual Meeting of the Ecological Society of Japan
    • Related Report
      2019 Annual Research Report
  • [Presentation] Arbuscular mycorrhizal fungi associated with the roots and soils of two Rhizophoraceae species2020

    • Author(s)
      Akaji Y., Inoue T., Taniguchi T., Baba S.
    • Organizer
      The 67th Annual Meeting of the Ecological Society of Japan
    • Related Report
      2019 Annual Research Report
  • [Presentation] 低酸素状態のマングローブ根圏で硝化は起きているのか?-植物と土壌微生物の相互作用2020

    • Author(s)
      森岡たまき、小山里奈、黒岩恵、井上智美、松尾奈緒子、大手信人
    • Organizer
      日本生態学会大会第67回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Capacity of two mangrove species, Bruguiera gymnorrhiza and Rhizophora stylosa, to use nitrate as a N source2020

    • Author(s)
      Koyama L.A., Inoue T., Matsuo N.
    • Organizer
      OrgN2020, International workshop on organic nitrogen and plant nutrition - from molecular mechanisms to ecosystems
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 日中および夜間の生育温度上昇に対するヤエヤマヒルギ実生の形態的・生理的変化2019

    • Author(s)
      赤路康朗、井上智美
    • Organizer
      日本生態学会第66回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] マングローブ植物への新たな窒素供給経路の検証―呼吸根通気システムと窒素固定菌2019

    • Author(s)
      井上智美、高津文人、下野綾子
    • Organizer
      日本生態学会第66回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] The Estimation of CO2 Flux in Subtropical Coastal Ecosystems Using a Numerical Model2018

    • Author(s)
      Moki H., Sohma A., Shibuki H., Toyoda K., Akhand A., Watanabe K., Tokoro T., Inoue T., Yamano H., Banno M., Nakagawa Y., Matsuda H., Kuwae T.
    • Organizer
      JpGU-AGU Joint Meeting 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 生育温度がヤエヤマヒルギ実生の生残・成長・光合成に与える影響2018

    • Author(s)
      赤路康朗、井上智美、冨松元、川西あゆみ
    • Organizer
      中国四国地区生物系三学会合同大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] マングローブ根圏に形成される窒素固定細菌群集2018

    • Author(s)
      下野綾子、井上智美
    • Organizer
      第一回環境DNA学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Conservation and evaluation of the functions of coastal ecosystems for ecosystem-based management of tropical and subtropical islands2017

    • Author(s)
      Yamano H., Inoue T
    • Organizer
      Environmental Scientists Network for Asia-Pacific Islands
    • Place of Presentation
      沖縄市町村自治会館(那覇)
    • Year and Date
      2017-03-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] マングローブ植物(メヒルギ)の呼吸特性2017

    • Author(s)
      井上智美, 川西あゆみ,冨松元
    • Organizer
      日本生態学会第64回大会
    • Place of Presentation
      早稲田大学(東京)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Carbon storage of mangrove ecosystems: estimated models and global maps applied their models2017

    • Author(s)
      Inoue T., Baba S., Ishihama F., Kawanishi A., Hayashi F., Ohshiro N., Kezuka M., Mochida Y., Chan H.T., Kainuma M., Kimura N., Yamano H., Kuwae T., Matsuda H.
    • Organizer
      International Conference on Sustainable Mangrove Ecosystems, Bali, Indonesia
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Function of mangrove plants-Roots and soil chemicals2017

    • Author(s)
      Inoue T.
    • Organizer
      JpGU-AGU Joint Meeting 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] More collaboration needed to protect Mangrove ecosystems.2016

    • Author(s)
      Inoue T., Baba S.
    • Organizer
      The International Symposium on Mangroves
    • Place of Presentation
      Seacology Sudeesa Mangrove Museum (Sri Lanka)
    • Year and Date
      2016-07-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Adaptation to climate change through mangrove rehabilitation involving local community participation.2016

    • Author(s)
      Baba S., Chan H.T., Kainuma M., Oshiro N., Kezuka M., Kimura N., Inoue T.
    • Organizer
      International Conference on Climate Change, Biodiversity and Ecosystem Services for the Sustainable Development Goals (SDGs)
    • Place of Presentation
      The Sirindhorn International Environmental Park (Thailand)
    • Year and Date
      2016-06-28
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] Blue carbon in Shallow Coastal Ecosystems: Carbon Dynamics, Policy, and Implementation2018

    • Author(s)
      Inoue T.
    • Total Pages
      373
    • Publisher
      Springer
    • Related Report
      2018 Annual Research Report
  • [Book] 熱帯・亜熱帯の森マングローブ,島谷幸宏他編,図説日本の湿地2017

    • Author(s)
      井上智美
    • Total Pages
      212
    • Publisher
      朝倉書店
    • Related Report
      2017 Annual Research Report

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Published: 2016-04-21   Modified: 2021-02-19  

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