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Studies on structure and properties of xylan which is a bottleneck on enzymatic saccharification of woody biomass

Research Project

Project/Area Number 16H04949
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Wood science
Research InstitutionThe University of Tokyo

Principal Investigator

Samejima Masahiro  東京大学, 大学院農学生命科学研究科(農学部), 教授 (30162530)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2018: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Keywords木質バイオマス / セルロース / ヘミセルロース / キシラン / リグニン / 酵素糖化
Outline of Final Research Achievements

When enzymatic saccharification was performed on ammonia-treated biomass derived from six species of hardwood, the amount of easily enzymatic degradable xylan, which might exists on the surface of cellulose, is large in birch wood biomass. On the contrary, it revealed that the amount of easily enzymatic degradable xylan was quit low in wood biomasses of Acacia and Eucalyptus. Consequently, in the later case, a large part of xylan might exist as an recalcitrant form against enzymatic saccharification in the complex with cellulose。 In addition, it was shown that the degree of substitution of the glucuronic acid residue attached on the xylan main chain is lower in the easily degradable xylan in birch wood biomass compared to the case of other species. Furthermore, in the preparation of holocellulose from hardwood, it was suggested that some part of xylan are incorporated into cellulose and it makes another bottleneck for enzymatic saccharification.

Academic Significance and Societal Importance of the Research Achievements

広葉樹由来の木質バイオマスにおいては、セルロースの表面に存在すると思われるキシランとセルロース構造の内部に取り込まれたと考えられるキシランが存在し、その比率は樹種間で大きな差があることが明らかとなった。また、キシラン主鎖に対するグルクロン酸残基の置換度とその分布が樹種によって大きく異なり、これらの相違が酵素糖化の効率に大きな影響を与えていることが示唆された。さらに、ホロセルロースの調製過程でグルクロン酸残基の脱離が起こることが明らかとなり、その結果によりキシランの一部が酵素糖化に対して難分解性となることが示された。以上は、新規の発見であり、その学術的ならびに応用的価値は高いと思われる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (16 results)

All 2019 2018 2017 2016

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (14 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results)

  • [Journal Article] Secretome analysis of the basidiomycete Phanerochaete chrysosporium grown on ammonia-treated lignocellulosic biomass from birch wood.2018

    • Author(s)
      Sakuragi S, Hori C, Igarashi K, Samejima M.
    • Journal Title

      J. Wood Sci.

      Volume: 64 Issue: 6 Pages: 845-853

    • DOI

      10.1007/s10086-018-1770-4

    • NAID

      40021752969

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Application of ammonia pretreatment to enable enzymatic hydrolysis of hardwood biomass2018

    • Author(s)
      Kiyoshi Sakuragi, Kiyohiko Igarashi, Masahiro Samejima
    • Journal Title

      Polymer Degradation and Stability

      Volume: 148 Pages: 19-25

    • DOI

      10.1016/j.polymdegradstab.2017.12.008

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 二種の糸状菌由来β-キシロシダーゼにおける構造と機能の比較2019

    • Author(s)
      小島圭輔、砂川直樹、五十嵐圭日子、鮫島正浩
    • Organizer
      第69回日本木材学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 糸状菌由来GH family3 β-キシロシダーゼの構造と反応特性の比較解析2018

    • Author(s)
      小島圭輔、砂川直樹、五十嵐圭日子、鮫島正浩
    • Organizer
      セルラーゼ研究会第32回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Xylan as another Recalcitrant Factor on Enzymatic Saccharification of Lignocellulosic Biomass2018

    • Author(s)
      Kiyoshi Sakuragi, Miki Kokubo, Kiyohiko Igrarashi, Masahiro Samejima
    • Organizer
      The 6th Asian Conference on Biomass Science
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Substrate Specificity and Kinetic Analysis of beta-Xylosidase from the basidiomycete Phanerochaete chrysosporium2018

    • Author(s)
      Keisuke Kojima, Naoki Sunagawa, Kiyohiko Igarashi, Masahiro Samejima
    • Organizer
      The 6th Asian Conference on Biomass Science
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 酵素の目を通してみたバイオマスとセルロースの姿2018

    • Author(s)
      鮫島正浩
    • Organizer
      セルロース学会関東支部ミニシンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Application of Ammonia Pretreatment towards Enzymatic Saccharification of Hardwood Biomass2018

    • Author(s)
      Kiyoshi Sakuragi, Kiyohiko Igarashi, Masahiro Samejima
    • Organizer
      The 5th Asian Conference on Biomass Science
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 担子菌Phanerochaete chrysosporiumが生産するα-L-アラビノフラノシダーゼの機能について2018

    • Author(s)
      木根啓太、砂川直樹、五十嵐圭日子、鮫島正浩
    • Organizer
      第68回日本木材学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 担子菌Phanerochaete chrysosporium由来β-xylosidaseの機能解析2017

    • Author(s)
      小島圭輔、砂川直樹、五十嵐圭日子、鮫島正浩
    • Organizer
      セルラーゼ研究会第31回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 脱リグニン処理が木質バイオマスの酵素糖化に与える影響2017

    • Author(s)
      櫻木潔, 五十嵐圭日子, 鮫島正浩
    • Organizer
      第12回バイオマス科学会議
    • Related Report
      2016 Annual Research Report
  • [Presentation] 木質バイオマスの酵素糖化に向けた木材腐朽菌Phanerochaete chrysosporiumの全分泌タンパク質解析2016

    • Author(s)
      櫻木潔, 古久保美樹, 堀千明, 石田卓也, 五十嵐圭日子, 鮫島正浩
    • Organizer
      セルラーゼ研究会第30回大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 担子菌由来α-L-アラビノフラノシダーゼの機能解析2016

    • Author(s)
      木根啓太, 砂川直樹, 石田卓也, 五十嵐圭日子, 鮫島正浩
    • Organizer
      セルラーゼ研究会第30回大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 木材腐朽菌Phanerochaete chrysosporiumの全分泌タンパク質解析に基づく木質バイオマスの酵素糖化2016

    • Author(s)
      櫻木潔, 古久保美樹, 堀千明, 石田卓也, 五十嵐圭日子, 鮫島正浩
    • Organizer
      第25回日本エネルギー学会大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Expression and characterization of beta-xylosidase from Phanerochaete chrysosporium2016

    • Author(s)
      Takuya Ishida, Michiko Maruyama, Kiyohiko Igrarashi, Masahiro Samejima
    • Organizer
      4th Asian Conference on Biomass Science
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ・Secrotome analysis of the extracellular proteins from Phanerochaete chrysosporium for enzymatic saccharification2016

    • Author(s)
      Kiyoshi Sakuragi, Miki Kokubo, Takuya Ishida, Kiyohiko Igarashi, Masahiro Samejima
    • Organizer
      4th Asian Conference on Biomass Science
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2021-01-27  

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