Production of useful aromatic compounds from lignin-derived aromatic compounds using biphenyl/PCB-degrading bacteria
Project/Area Number |
16H04953
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Wood science
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Research Institution | Kyoto University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
藤原 秀彦 別府大学, 公私立大学の部局等, 准教授 (10435167)
|
Project Period (FY) |
2016-04-01 – 2019-03-31
|
Project Status |
Completed (Fiscal Year 2018)
|
Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
|
Keywords | リグニン / ビフェニル / PCB / プロテオミクス / ゲノミクス |
Outline of Final Research Achievements |
A variety of microorganisms play a significant role in the mineralization of plant lignin. We have isolated a number of biphenyl/PCB-degrading bacteria from various environmental samples and studied the degradation modes from the biochemical and genetic bases. In this study, we found that some biphenyl/PCB-degrading bacteria can grow well on lignin-derived aromatic compounds as the sole sources of carbon and energy. Using gel-based proteomics, we found that many proteins responsible for the metabolism of aromatic compounds are inducibly expressed in a Pseudomonas strain. We also cloned the lignin peroxidase genes from a Rhodococcus strain and expressed them. The results show that using proteomic and genomic approaches, we can efficiently screen and identify candidate proteins and genes involved in the metabolism of lignin-derived aromatic compounds from biphenyl/PCB-degrading bacteria. These approaches can also lead to the production of useful aromatic compounds from wood biomass.
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Academic Significance and Societal Importance of the Research Achievements |
本研究では,難分解性芳香族化合物の分解系を有する環境汚染物質分解細菌(ビフェニル/PCB 分解細菌)を用いてリグニンやその分解生成物から有用な芳香族化合物生産の基盤構築を目指した.今回は,ビフェニル/PCB 分解細菌のゲノム情報等を最大限に活用しつつ,プロテオミクスの手法も駆使し,有用な芳香族化合物生産のための酵素や遺伝子をスクリーニングすることができた.これらの成果は,「分解菌を生産菌に変える」という菌の育種を通じて新たな芳香族化合物の分解代謝系の構築に繋がる.さらには,リグニン由来の芳香族化合物の機能性バイオマテリアルの開発や新しいリグニンビジネスの創出への一助となる.
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Report
(4 results)
Research Products
(21 results)
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[Journal Article] Pseudomonas furukawaii sp. nov., a polychlorinated biphenyl-degrading bacterium isolated from biphenyl-contaminated soil in Japan2018
Author(s)
Kimura, N., Watanabe, T., Suenaga, H., Fujihara, H., Futagami, T., Goto, M., Hanada, S., Hirose, J.
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Journal Title
Int. J. Syst. Evol. Microbiol.
Volume: 68
Issue: 5
Pages: 1429-1435
DOI
Related Report
Peer Reviewed
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[Journal Article] Preparation of intracellular proteins from a white-rot fungus surrounded by polysaccharide sheath and optimization of their two-dimensional electrophoresis for proteomic studies2017
Author(s)
Watanabe, T., Yoshioka, K., Kido, A., Lee, J., Akiyoshi, H., and Watanabe, T.
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Journal Title
J. Microbiol. Methods
Volume: 142
Pages: 63-70
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Insights into the genomic plasticity of Pseudomonas putida KF715, a strain with unique biphenyl-utilizing activity and genome instability properties2017
Author(s)
Suenaga, H., Fujihara H., Kimura, N., Hirose, J., Watanabe, T., Futagami, T., Goto, M., Shimoda, J., and Furukawa K.
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Journal Title
Environ. Microbiol. Rep.
Volume: 9
Issue: 5
Pages: 589-598
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Complete genome sequence of the polychlorinated biphenyl-degrading bacterium Pseudomonas putida KF715 (NBRC 110667) isolated from biphenyl-contaminated soil2017
Author(s)
Suenaga, H., Yamazoe, A., Hosoyama, A., Kimura, N., Hirose, J., Watanabe, T., Fujihara, H., Futagami, T., Goto, M., Furukawa, K.
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Journal Title
Genome announcements
Volume: 5
Issue: 7
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Insight into genome plasticity of Pseudomonas putida KF715 which has unique properties in biphenyl-utilizing activity and genome instability2018
Author(s)
Suenaga, H., Fujihara, H., Kimura, N., Hirose, J., Watanabe, T., Futagami, T., Goto, M., Furukawa, K.
Organizer
17th International Symposium on Microbial Ecology
Related Report
Int'l Joint Research
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