| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2018: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2016: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
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| Outline of Final Research Achievements |
A functional screening of fungal cytochromes P450 for combinatorial biosynthesis of triterpenoids was conducted. To elucidate catalytic potentials of fungal P450s against plant triterpenoids, we generated recombinant yeasts that allow co-expression of fungal P450 and plant triterpene synthase. Based upon metabolic studies, we identified fungal P450s showing catalytic activities for plant triterpenoids lupeol and beta-amyrin, indicating a series of plant-like triterpenoids could be produced using fungal P450s. In addition, some basidiomycetous P450s were suggested to be capable of decorating steroid compounds appeared from steroid metabolism by yeast. These results suggested that basidiomycetes possess P450-dependent metabolic systems to generate various ergostane-type steroids.
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