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Development of the selection method using the virus disease resistance gene in natural population.

Research Project

Project/Area Number 16H04970
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Aquatic bioproduction science
Research InstitutionTokyo University of Marine Science and Technology

Principal Investigator

Sakamoto Takashi  東京海洋大学, 学術研究院, 教授 (40313390)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Fiscal Year 2019: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2017: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywordsヒラメ / リンホシスチス / 耐病性 / ゲノム / 育種 / 遺伝子 / 養殖 / 選抜 / ウイルス病 / 責任遺伝子 / 遺伝子選抜
Outline of Final Research Achievements

Japanese flounder is an economically important aquaculture species in Asian countries such as Japan, Korea and China. Lymphocystis disease (LD) is one of important disease and causes serious damage to Japanese flounder aquaculture industry. In this study, we investigated the virus disease resistant gene for LD and tried to develop the selection method using the sequences of the gene in natural population.
This research mainly accomplished three things as follows, 1) the development of the genotyping method with short-term rearing in wild juvenile fish, 2) performing the genome wide association study of the disease resistant trait on wild fish, 3) the finding of SNPs related with the disease resistant trait. These results would be very useful information to have the evidence of Lymphocystis disease resistant gene in Japanese flounder.

Academic Significance and Societal Importance of the Research Achievements

本研究では、リンホシスチス病に対する耐病性責任遺伝子の同定を試みると共に、その責任遺伝子を用いた天然魚からの選抜技術の開発を行った。天然集団の中にリンホシスチス耐病性形質を保持する個体が少ないため、まだ統計的に有意な結果にはならなかったが、相関解析などの成果からこれまでの耐病性責任遺伝子候補がやはり有力な遺伝子候補であることが明らかになった。本研究成果により、天然魚において耐病性責任遺伝子を用いて直接的に耐病性個体を選抜し、新規耐病性系統を作れる可能性を示したと考えている。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (5 results)

All 2018 2017 2016

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results,  Invited: 3 results)

  • [Journal Article] The genome and transcriptome of Japanese flounder provide insights into flatfish asymmetry.2017

    • Author(s)
      C. Shao et al.,
    • Journal Title

      Nature Genetics.

      Volume: 49 (1) Issue: 1 Pages: 119-127

    • DOI

      10.1038/ng.3732

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 養殖魚類におけるゲノム情報を活用した育種研究2018

    • Author(s)
      坂本 崇
    • Organizer
      日本分析化学会 受託分析研究懇談会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] SNP detection within the region related to lymphocystis disease resistance in Japanese flounder Paralichthys olivaceus2017

    • Author(s)
      Saki Kabayama, Ayana Nakajima, Masatoshi Nakamoto, Tsubasa Uchino and Takashi Sakamoto
    • Organizer
      The JSFS 85th Anniversary-Commemorative International Symposium "Fisheries Science for Future Generations"
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 国内外の水産養殖における 育種の現状と将来展望について2017

    • Author(s)
      坂本 崇
    • Organizer
      第42回 全国養鱒技術協議会大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 養殖魚類における遺伝情報を活用した育種研究の現状と展望2016

    • Author(s)
      坂本 崇
    • Organizer
      第14回 種苗生産技術交流会
    • Place of Presentation
      広島県尾道市
    • Year and Date
      2016-08-09
    • Related Report
      2016 Annual Research Report
    • Invited

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Published: 2016-04-21   Modified: 2021-02-19  

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