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Creation of Molecular Biotechnology Basis for Marine Vibrios Toward Innovation of Marine Biohydrogen Production

Research Project

Project/Area Number 16H04976
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Aquatic life science
Research InstitutionHokkaido University

Principal Investigator

Sawabe Tomoo  北海道大学, 水産科学研究院, 教授 (30241376)

Co-Investigator(Kenkyū-buntansha) 美野 さやか  北海道大学, 水産科学研究院, 助教 (00755663)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2016: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Keywords海洋微生物 / マリンバイオマス / 水素 / 再生可能エネルギー / 水産学 / 微生物 / バイオマス / ゲノム / バイオエネルギー
Outline of Final Research Achievements

Aims of the study are to understand physiology and genomics of hydrogen production marine vibrios. We did achieve 1) optimized synthetic medium, 2) physiological and genome comparison among hydrogen producing marine vibrios, 3) a RNA-Seq, and 4) effects of nitrogen fixation on hydrogen production. We also discovered a new marine vibrio which is capable of producing hydrogen from alginate.

Academic Significance and Societal Importance of the Research Achievements

水素社会の形成に向けて水素生成技術基盤の強固化が求められている。研究代表者のグループで見いだした新規マリンビブリオは,海藻に含まれるユニークな炭水化物群を効率よく水素化する。海洋バイオマスは地政学的制限の少ない資源であり,日本沿岸の推定現存量は極めて多い。これら資源をバイオ水素の生産に向けることができれば,日本の工業的水素生産に貢献できる可能性がある。本研究では,マリンバイオマスを原料にバイオ水素を生成する微生物が有する水素生成分子マシナリーの細胞生理学的特性と遺伝子発現制御の解明を行った。

Report

(4 results)
  • 2019 Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (12 results)

All 2019 2018 2017 2016

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (8 results) Book (1 results)

  • [Journal Article] Understanding the NaCl-dependent behavior of hydrogen production of a marine bacterium, Vibrio tritonius2019

    • Author(s)
      N. Al-saari, E. Amada, Y. Matsumura, M. Tanaka, S. Mino, T. Sawabe
    • Journal Title

      PeerJ

      Volume: 7 Pages: e6769-e6769

    • DOI

      10.7717/peerj.6769

    • NAID

      120006633302

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Availability of Nanopore sequences in the genome taxonomy for Vibrionaceae systematics: Rumoiensis clade species as a test case2018

    • Author(s)
      T. Mami, S. Mino, Y. Ogura, T. Hayashi, T. Sawabe
    • Journal Title

      PeerJ

      Volume: 6 Pages: e5018-e5018

    • DOI

      10.7717/peerj.5018

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Vibrio aphrogenes sp. nov., in the Rumoiensis clade isolated from a seaweed2017

    • Author(s)
      M. Tanaka, S. Mino, T. Sawabe et al.
    • Journal Title

      PLoS ONE

      Volume: 12 Issue: 6 Pages: e0180053-e0180053

    • DOI

      10.1371/journal.pone.0180053

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] マリンビブリオを生物触媒とした連続的水素生産2018

    • Author(s)
      宮坂信城・美野さやか・澤辺智雄
    • Organizer
      マリンバイオテクノロジー学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Comparative Genomics of Ecophysiologically Unique Rumoiensis Clade Species, Vibrionaceae2018

    • Author(s)
      M. Tanaka, S. Mino, T. Sawabe et al.
    • Organizer
      日本微生物生態学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 水素生成ビブリオVibrio aphrogenesのゲノムの特徴2018

    • Author(s)
      田仲真実, 美野さやか, 澤辺智雄
    • Organizer
      第52回ビブリオシンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] マリンビブリオを生物触媒とした連続的水素生産システムの開発2017

    • Author(s)
      宮坂信城・美野さやか・澤辺智雄
    • Organizer
      日本生物工学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] アルギン酸から水素を生成可能な新規ビブリオ属細菌の分類学的検討2017

    • Author(s)
      田仲真実・美野さやか・澤辺智雄ら
    • Organizer
      マリンバイオテクノロジー学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Vibrio tritonius AM2株のギ酸水素リアーゼ遺伝子群のトランスクリプトーム解析2016

    • Author(s)
      水越草太・松村祐太・N. Al-saari・美野さやか・澤辺智雄・澤辺桃子
    • Organizer
      平成28年度日本水産学会秋季大会
    • Place of Presentation
      近畿大学農学部(奈良県、奈良市)
    • Year and Date
      2016-09-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] マリンビブリオを生物触媒としたアルギン酸の水素変換2016

    • Author(s)
      天田愛梨・松村祐太・N. Al-saari・美野さやか・澤辺智雄・澤辺桃子
    • Organizer
      平成28年度日本水産学会秋季大会
    • Place of Presentation
      近畿大学農学部(奈良県、奈良市)
    • Year and Date
      2016-09-08
    • Related Report
      2016 Annual Research Report
  • [Presentation] A strategic approach for optimizing the fermentative hydrogen production of Vibrio tritonius AM2T using Design of Experiment2016

    • Author(s)
      N. Al-saari, S. Mino, T. Sawabe
    • Organizer
      第18回マリンバイオテクノロジー学会大会
    • Place of Presentation
      北海道大学函館キャンパス(北海道、函館市)
    • Year and Date
      2016-05-28
    • Related Report
      2016 Annual Research Report
  • [Book] Molecular Diversity of Environmental Prokaryotes2016

    • Author(s)
      N. Al-saari, Tomoo Sawabe, Y. Marsumura, K. Sato, S. Mino, Toko Sawabe
    • Total Pages
      379
    • Publisher
      CRC Press
    • Related Report
      2016 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2021-02-19  

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