Control and anomaly detection algorithm for methane fermentation by using information of reaction intermediate and microbial fuel cell

• Project/Area Number: 16H05003
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Agricultural environmental engineering/Agricultural information engineering
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: TOJO SEISHU 東京農工大学, (連合)農学研究科(研究院), 教授 (40155495)
• Co-Investigator(Kenkyū-buntansha): 帖佐 直 東京農工大学, (連合)農学研究科(研究院), 准教授 (10355597)
• Research Collaborator: KOBAYASHI Kaoru ; UEDA Megumi ; KURIBAYASHI Mayu ; CHOIRON Miftahul ; LIU Yitec
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2016: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
• Keywords: メタン発酵 / バイオガス生産 / 中間生成物 / pH / 微生物電池 / バイオフィルム / 有機酸 / pH緩衝能 / 水素－メタン2段発酵 / 発酵液pH / pHバッファ / アノード電極 / 発電 / 再生可能エネルギー / 異常検知 / メタン発酵消化液 / プロピオン酸 / クーロン効率 / 励起蛍光マトリックス / 発酵制御

Outline of Final Research Achievements

Three dimensional fluorescence analysis was employed to extract the information of intermediate product in methane fermentation process for recognizing fermentation state serially. Tyrosine, tryptophan and humic acid oriented component obtained from the information of wave length and size of the peak fluorescence are reasonable indicators for the above mentioned purpose. Microbial fuel cell demonstrated to perform consuming propionate in fermentation solution and generating electricity. An appropriate culturing method for anode biofilm showed high Coulomb efficiency of microbial fuel cell. Reduction of pH of fermentation solution caused with the accumulation of organic acids was controlled by using high buffer capacity of combined raw materials.

Academic Significance and Societal Importance of the Research Achievements

従来のメタン発酵の状態把握は、発酵液のpHや生成ガスの成分割合等で行われてきた。小型のプラントでは、有機酸の蓄積が進んでpHが低下し、発酵が酸敗に至ることがあった。このような変化をより事前に把握するため、メタン発酵過程の中間生成物に着目した。3次元蛍光分光分析を用いることで、実際にpHが低下するよりも早い時期に、pHの推移に影響を及ぼす物質の動態を観測できることが示された。
また、微生物電池は発酵過程で蓄積するプロピオン酸等を消費して発電することが実証され、メタン発酵の制御に微生物電池を組み入れた新たな発酵制御法の可能性が見いだされた。

Research Products

• [Presentation] Natural Buffer Effect on Biohydrogen Production Using Hot Compressed Water Pretreatment (2019)
  • Author(s): Miftahul Choiron, Seishu Tojo, Tadashi Chosa
  • Organizer: XXXVIII CIOSTA & CIGR V International Conference
  • Int'l Joint Research
• [Presentation] Consumption characteristics of propionate in anaerobic digestate using microbial fuel cells (2018)
  • Author(s): Megumi Ueda, Tadashi Chosa, Seishu Tojo
  • Organizer: XIX. World Congress of the International Commission of Agriculture and Biosystems Engineering (CIGR)
  • Int'l Joint Research
• [Presentation] 光合成細菌を用いた水素生産における基質濃度の影響 (2017)
  • Author(s): 野村環、東城清秀、帖佐直
  • Organizer: 第76回農業食料工学会年次大会
• [Presentation] Developing a New Technology for the Two Phase Methane Fermentation Sludge Recirculation Process (2017)
  • Author(s): Mayu Kuribayashi, Seishu Tojo, Tadashi Chosa, Tetsuo Murayama, Kouji Sasaki, Hiroshi Kotaka
  • Organizer: XXXVII CIOSTA, Research and Innovation for the Sustainable and Safe Management of Agricultural and Forestry Systems (CIOSTA2017), 13-15 June, 2018, Palermo, Italy
  • Int'l Joint Research
• [Presentation] Culturing Nitrifying Bacteria in Hydroponic Culture Solution to Enable Nitrification of Anaerobic Digestate (2016)
  • Author(s): Mayu Kuribayashi, Tadashi Chosa, Seishu Tojo
  • Organizer: 8th International Symposium on Machinery and Mechatronics for Agricultural and Biosystems Engineering (ISMAB 2016), Toki Messe, Niigata, Japan. 23-25 May, 2016.
  • Int'l Joint Research
• [Presentation] Electricity Generation Characteristics of Microbial Fuel Cell Using Substrate of Volatile Fatty Acids from Hydrogen Fermentation (2016)
  • Author(s): Megumi Ueda, Takayuki Ichioka, Tadashi Chosa, Seishu Tojo
  • Organizer: 8th International Symposium on Machinery and Mechatronics for Agricultural and Biosystems Engineering (ISMAB 2016), Toki Messe, Niigata, Japan. 23-25 May, 2016.
  • Int'l Joint Research
• [Patent(Industrial Property Rights)] 水素生成複合微生物群の優占化方法、水素発酵方法 (2017)
  • Inventor(s): (株)トッププランニングJAPAN・（国）東京農工大学
  • Industrial Property Rights Holder: (株)トッププランニングJAPAN・（国）東京農工大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2017