| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2018: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2016: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
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| Outline of Final Research Achievements |
The present study aimed to elucidate the neural mechanisms controlling estrus behavior in ruminants using genetically modified goats that can conditionally knock out estrogen receptor (ER) expression, which plays a primary role in inducing estrus behavior. First, we constructed a model that induced estrus behavior by hormone treatment and found that the increase in the behavioral activity associated with estrus was a quantitative parameter to evaluate the estrus behavior in goats. Second, we identified the nucleotide sequence and deduced amino acid sequence of the goat ER gene mRNA, and optimized the goat genetic engineering technology to implement genome editing technology by “gene editing by electroporation of Cas9 protein (GEEP)” method in 1-cell stage embryo. Third, we developed a method to implant a microinjection cannula stereotaxically in the brain region where goat ER is highly expressed.
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