Comprehensive analysis for responsible genes associated with fatty acid composition in cattle

• Project/Area Number: 16H05015
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Animal production science
• Research Institution: Kobe University
• Principal Investigator: Mannen Hideyuki 神戸大学, 農学研究科, 教授 (20263395)
• Co-Investigator(Kenkyū-buntansha): 小林 栄治 国立研究開発法人農業・食品産業技術総合研究機構, 畜産研究部門, ユニット長 (00186727) ; 本多 健 神戸大学, 農学研究科, 助教 (10432551) ; 笹崎 晋史 神戸大学, 農学研究科, 准教授 (50457115) ; 大山 憲二 神戸大学, 農学研究科, 教授 (70322203)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000); Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2018: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2016: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
• Keywords: ウシ / 黒毛和種 / 脂肪酸組成 / 責任遺伝子 / 全ゲノムリシーケンス / 関連解析 / 育種

Outline of Final Research Achievements

In this study, we aimed to identify the responsible gene associated with fatty acid composition in Japanese Black cattle. We performed genome wide association analysis for Hyogo Japanese Black cattle, and then identified candidate chromosomal regions in BTA9 and 14. Whole genome resequencing and gene functional analysis detected 3 candidate genes associated with fatty acid composition, CYB5R4, MED23 and VNN1. By population association study, CYB5R4 c.*349G>T revealed highest significant value. Therefore, CYB5R4 c.*349G>T is most candidate variant as responsible for fatty acid composition in Hyogo Japanese Black cattle population.

Academic Significance and Societal Importance of the Research Achievements

牛肉の脂肪酸組成は消費者の健康や高品質化を考えた場合重要な形質である。本研究課題では、ゲノムワイド関連解析、全ゲノムリシーケンス解析、Pathway解析など、ゲノミクスの先端技術を適用することにより、新規の責任遺伝子候補を明らかにすることができた（CYB5R4 c.*349G>T）。この遺伝子多型をDNAマーカーとして利用することにより、脂肪酸組成のコントロールが可能となり、より美味しく・健康的な牛肉生産に貢献することができる。

Research Products

• [Journal Article] Effect of DNA markers on the fertility traits of Japanese Black cattle for improving beef quantity and quality. (2020)
  • Author(s): Kawaguchi F, Tsuchimura M, yama K, Matsuhashi T, Maruyama S, Mannen H, Sasazaki S.
  • Journal Title: Archives Animal Breeding Volume: 63 Issue: 1 Pages: 9-17
  • DOI: 10.5194/aab-63-9-2020
  • NAID: 120006784495
  • Peer Reviewed
• [Journal Article] The non-synonymous mutation in bovine SPP1 gene influences carcass weight. (2019)
  • Author(s): Matsumoto H, Kohara R, Sugi M, Usui A, Oyama K, Mannen H, Sasazaki S.
  • Journal Title: Heliyon Volume: 5 Issue: 12 Pages: e03006-e03006
  • DOI: 10.1016/j.heliyon.2019.e03006
  • Peer Reviewed
• [Journal Article] Effect of STARD3 gene polymorphism on carcass traits and fatty acid composition in Japanese Black cattle. (2019)
  • Author(s): Kigoshi H, Kawaguchi F, Oyama K, Mannen H, Shinji Sasazaki.
  • Journal Title: J. Anim. Genet. Volume: 47 Pages: 37-41
  • NAID: 130007701688
  • Peer Reviewed
• [Journal Article] The SNPs in bovine MMP14 promoter influence on fat-related traits. (2019)
  • Author(s): Matsumoto H, Kawaguchi F, Itoh S, Yotsu S, Fukuda K, Oyama K, Mannen H, Sasazaki S.
  • Journal Title: Meta Gene. Volume: 20 Pages: 100558-100558
  • DOI: 10.1016/j.mgene.2019.100558
  • Peer Reviewed
• [Journal Article] Whole-genome resequencing to identify candidate genes for the QTL for oleic acid percentage in Japanese Black cattle. (2019)
  • Author(s): Kawaguchi F, Kigoshi H, Fukushima M, Iwamoto E, Kobayashi E, Oyama K, Mannen H, Sasazaki S.
  • Journal Title: Anim Sci J. Volume: 90 Issue: 4 Pages: 467-472
  • DOI: 10.1111/asj.13179
  • NAID: 120007026340
  • Peer Reviewed
• [Journal Article] PLAG1 gene polymorphism on oleic acid percentage in various Japanese Black cattle populations (2018)
  • Author(s): Kigoshi H, Kawaguchi F, Yasuzumi R, Oyama K, Mannen H, Shinji Sasazaki
  • Journal Title: Genetics and Molecular Research Volume: 17 Issue: 3
  • DOI: 10.4238/gmr18056
  • Peer Reviewed
• [Journal Article] Pool-based genome-wide association study identified novel candidate regions on BTA9 and 14 for oleic acid percentage in Japanese Black cattle (2018)
  • Author(s): Kawaguchi F, Kigoshi H, Nakajima Ayaka, Matsumoto Y, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Kobayashi E, Honda T, Oyama K, Mannen H, Sasazaki S
  • Journal Title: Anim. Sci. J Volume: 89 Issue: 8 Pages: 1060-1066
  • DOI: 10.1111/asj.13035
  • Peer Reviewed
• [Journal Article] Pool-based genome-wide association study identified a VNN1 gene mutation T66M for oleic acid percentage in Japanese Black cattle (2018)
  • Author(s): Kawaguchi F, Kigoshi H, Nakajima Ayaka, Matsumoto Y, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Kobayashi E, Honda T, Oyama K, Mannen H, Sasazaki S
  • Journal Title: Anim. Sci. J. Volume: 印刷中
  • Peer Reviewed / Open Access
• [Journal Article] Identification of leptin gene polymorphisms associated with carcass traits and fatty acid composition in Japanese Black cattle (2017)
  • Author(s): Kawaguchi Fuki、Okura Kazuki、Oyama Kenji、Mannen Hideyuki、Sasazaki Shinji
  • Journal Title: Anim. Sci. J. Volume: 88 Issue: 3 Pages: 433-438
  • DOI: 10.1111/asj.12672
  • NAID: 120006382451
  • Peer Reviewed / Open Access
• [Journal Article] 黒毛和種集団におけるLPL遺伝子内多型の脂肪酸組成に対する効果 (2017)
  • Author(s): 川口芙岐・古賀聡・大山憲二・万年英之・笹崎晋史
  • Journal Title: 動物遺伝育種研究 Volume: 45 Pages: 3-8
  • NAID: 130005440020
  • Peer Reviewed
• [Presentation] Effect of the PLAG1 gene polymorphism on carcass weight and oleic acid percentage in Japanese Black cattle populations (2019)
  • Author(s): Kawaguchi F;Kigoshi H;Yasuzumi R;Oyama K;Mannen H;Sasazaki S
  • Organizer: 37th International Society for Animal Genetics Conference
  • Int'l Joint Research
• [Presentation] Validation of candidate SNPs for fat percentage in bovine rib-eye area using Integrated Fluidic Circuit (2019)
  • Author(s): Yamamoto R;Toyomoto S;Kohama N;Akiyama T;Yoshida E;Kobayashi E;Oyama K;Mannen H;Sasazaki S
  • Organizer: 37th International Society for Animal Genetics Conference
  • Int'l Joint Research
• [Presentation] ウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索 (2018)
  • Author(s): 川口芙岐、木越洸利、小浜菜美子、秋山敬孝、福島護之、吉田恵実、小林栄治、大山憲二、万年英之、笹崎晋史
  • Organizer: 第124回日本畜産学会大会
• [Presentation] Detection of candidate polymorphisms in the QTL for oleic acid percentage on BTA9 based on whole-genome resequencing data (2018)
  • Author(s): Kawaguchi F, Kigoshi H, Kohama N, Kohama N, Akiyama T, Fukushima M, Yoshida E, Kobayashi E, Oyama K, Mannen H, Sasazaki S
  • Organizer: PAG Asia 2018
  • Int'l Joint Research
• [Presentation] 兵庫県黒毛和種集団における全ゲノムリシーケンスデータを用いた枝肉形質に対する候補変異の抽出 (2018)
  • Author(s): 木越洸利、川口芙岐、大山憲二、万年英之、笹崎晋史
  • Organizer: 第18回日本動物遺伝育種学会大会
• [Presentation] ウシ全ゲノムリシーケンスデータを用いたオレイン酸含有率に対する責任変異の探索 (2018)
  • Author(s): 川口芙岐、木越洸利、小浜菜美子、秋山敬孝、福島護之、吉田恵実、小林栄治、大山憲二、万年英之、笹崎晋史
  • Organizer: 第124回日本畜産学会大会, 東京
• [Presentation] Association of VNN1 gene polymorphism with fatty acid composition in Japanese Black cattle (2017)
  • Author(s): Kigoshi H, MatsumotoY, Kawaguchi F, Uemoto Y, Kobayashi E, Fukushima M, Iwamoto E, Yoshida E, Akiyama T, Kohama N, Oyama K, Honda T, Mannen H, Sasazaki S
  • Organizer: 36th International Society for Animal Genetics Conference, Dublin, Ireland
  • Int'l Joint Research
• [Presentation] Effects of DNA markers associated with carcass traits and fatty acid composition on fertility traits in Japanese Black cows (2017)
  • Author(s): Tsuchimura M, Fukazawa K, Kawaguchi F, Matsuhashi T, Maruyama S, Oyama K, Sasazaki S, Mannen H
  • Organizer: 36th International Society for Animal Genetics Conference, Dublin, Ireland
  • Int'l Joint Research
• [Presentation] Genome-wide association study revealed a candidate region for beef marbling on BTA7 (2017)
  • Author(s): Sasazaki S, Nakajima T, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Kobayashi E, Oyama K, Mannen H
  • Organizer: 36th International Society for Animal Genetics Conference, Dublin, Ireland
  • Int'l Joint Research
• [Presentation] PLAG1 polymorphism (ss319607405) is associated with oleic acid percentage in Japanese Black cattle (2017)
  • Author(s): Kawaguchi F, Kigoshi H, Nakajima A, Matsumoto Y, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Kobayashi E, Honda T, Oyama K, Mannen H, Sasazaki S
  • Organizer: 36th International Society for Animal Genetics Conference, Dublin, Ireland
  • Int'l Joint Research
• [Presentation] 兵庫県黒毛和種におけるゲノムワイド関連解析により同定されたVNN1遺伝子T66M多型のオレイン酸含有率に対する効果 (2017)
  • Author(s): 木越洸利、川口芙岐、上本吉伸、福島護之、吉田恵実、岩本英治、秋山敬孝、小浜菜美子、小林栄治、大山憲二、万年英之、笹崎晋史
  • Organizer: 第18回日本動物遺伝育種学会大会, 厚木
• [Presentation] 複数の黒毛和種集団を用いたPLAG1遺伝子多型のオレイン酸含有率に対する効果の検討 (2017)
  • Author(s): 川口芙岐、木越洸利、安栖梨理、小浜菜美子、秋山敬孝、福島護之、小林栄治、大山憲二、万年英之、笹崎晋史
  • Organizer: 第18回日本動物遺伝育種学会大会, 厚木
• [Presentation] 黒毛和種繁殖雌牛集団におけるレプチン遺伝子内多型と繁殖形質との関連 (2016)
  • Author(s): 川口芙岐、深澤謙治、小浜菜美子、秋山敬孝、福島護之、小林栄治、大山憲二、万年英之、笹崎晋史
  • Organizer: 第17回日本動物遺伝育種学会大会
  • Place of Presentation: 名古屋大学（愛知県・名古屋市）
  • Year and Date: 2016-11-05
• [Presentation] The origin, immigration roots and genetic diversity of Japanese Wagyu (2016)
  • Author(s): Mannen, H.
  • Organizer: The 1st Symposium in Jeju Black cattle Research Center: Genetics & Breeding and Mass Production of Domestic Animal using Genome Analysis
  • Place of Presentation: Jeju Island, Korea
  • Year and Date: 2016-10-20
  • Int'l Joint Research / Invited
• [Presentation] The origin and genetic diversity of Japanese Wagyu (2016)
  • Author(s): Mannen, H.
  • Organizer: 17th AAAP Animal Science Congress
  • Place of Presentation: ホテル日航福岡（福岡県・福岡市）
  • Year and Date: 2016-08-22
  • Int'l Joint Research / Invited
• [Presentation] Identification of polymorphisms associated with oleic acid percentage by pool-based genome wide association study (GWAS) in Japanese Black (2016)
  • Author(s): Kawaguchi F, Nakajima A, Matsumoto Y, Uemoto Y, Fukushima M, Yoshida E, Iwamoto E, Akiyama T, Kohama N, Kobayashi E, Honda T, Oyama K, Mannen H, Sasazaki S
  • Organizer: 35th International Society for Animal Genetics Conference
  • Place of Presentation: Salt Lake City, UT、USA
  • Year and Date: 2016-07-23
  • Int'l Joint Research
• [Book] 動物遺伝育種学 (2017)
  • Author(s): 万年英之 他
  • Total Pages: 204
  • Publisher: 朝倉書店