Development of an evaluation system for anti-disease immunobiotics of livestock and its applied strategy
Project/Area Number |
16H05019
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Animal production science
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Research Institution | Tohoku University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
大坪 和香子 東北大学, 農学研究科, 助教 (00598203)
麻生 久 東北大学, 農学研究科, 教授 (50241625)
齋藤 忠夫 東北大学, 農学研究科, 教授 (00118358)
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Project Period (FY) |
2016-04-01 – 2020-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2018: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2017: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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Keywords | 畜産学 / イムノバイオティクス / 免疫学 / 微生物 / 評価系 / 動物 / 食品 / 健全育成 / 飼料 / ウイルス / プロバイオティクス |
Outline of Final Research Achievements |
An in vitro unique anti-disease selection and evaluation system for the livestock-responsive immunobiotics (mucosal immunomodulatory functional probiotics) was developmentally established, and the basic technology for the livestock healthy growth strategy was established via the development and use of this system. Specifically, an infection system for bacterial, viral, and other enteric pathogens was established using intestinal epithelial cell lines derived from livestock, and an evaluation system was constructed using the ability to protect against infection as an parameter. In addition, the evaluation system selected anti-disease immunobiotics and validated the usefulness of this system by in vivo confirmation study.
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Academic Significance and Societal Importance of the Research Achievements |
本研究成果により、家畜に応用可能な抗病性イムノバイオティクスの的確な選抜・評価が可能となり、家畜健全育成技術の向上と抗病性機構解明が飛躍的に進む。それにより、薬剤のみに頼らない安全な畜産食品生産の拡大と、関連学問・産業への波及効果が大いに期待できる。
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Report
(4 results)
Research Products
(57 results)
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[Journal Article] Draft Genome Sequence of Weissella viridescens UCO-SMC3, Isolated from the Slime of Helix aspersa Muller Snails2019
Author(s)
Garcia-Cancino, A., L. Albarracin, M. Espinoza-Monje, J. Campos-Martin, V. Garcia-Castillo, Y. Nakano, W. Ikeda-Ohtsubo, C. Guitierrez-Zamorano, H. Morita, H. Kitazawa, J. Villena
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Journal Title
Microbiol. Res. Announc.
Volume: 8:11(e01654-18)
Issue: 11
Pages: 1-2
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Draft Genome Sequence of probiotic Lactobacillus brevis TUCO-5E isolated from porcine milk2018
Author(s)
Quilodran-Vega, S., L., Albarracin, E., Hebert, L. Saavedra, A. Fonseca, A. S. Burgos, H, Kitazawa, J. Villena
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Journal Title
Microbiol. Res. Announc,
Volume: 7:19(e01239-18)
Issue: 19
Pages: 1-2
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Genomic characterization of Lactobacillus delbrueckii TUA4408L and evaluation of the antiviral activities of its extracellular polysaccharides in porcine intestinal epithelial cells2018
Author(s)
Kanmani, P., L. Albarracin, H. Kobayashi, E. M. Hebert, L. Saavedra, R. Komatsu, B. Gatica, A. S. Burgos, A. Miyazaki, W. Ikeda-Ohtsubo, Y. Suda, H. Aso, S. Egusa, T. Mishima, H. Takahashi, J. Villena, H. Kitazawa
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Journal Title
Front. Immunol.
Volume: 9:2178
Pages: 1-16
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Exopolysaccharides from Lactobacillus delbrueckii OLL1073R-1 modulate innate antiviral immune response in porcine intestinal epithelial cells2017
Author(s)
Paulraj Kanmani, Leonardo Albarra2018.00326in, Hisakazu Kobayashi, Hikaru Iida, Ryoya Komatsu, A.K.M. Humayun Kober, Wakako Ikeda-Ohtsubo, Yoshihito Suda, Hisashi Aso, Seiya Makino, Hiroshi Kano, Tadao Saito, JulioVillen, Haruki Kitazawa
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Journal Title
Molecular Immunology
Volume: 93
Pages: 253-265
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Development of an in vitro immunobiotic evaluation system against rotavirus infection in bovine intestinal epitheliocytes2017
Author(s)
Kobayashi, H., P. Kanmani, T. Ishizuka, A. Miyazaki, J. Soma, L. Albarracin, Y. Suda, T. Nochi, H. Aso, N. Iwabuchi, J-z. Xiao, T. Saito, J. Villena, H. Kitazawa
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Journal Title
Beneficial Microbes
Volume: 8 (2)
Issue: 2
Pages: 309-321
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Transcriptomic analysis of the innate antiviral immune response in porcine intestinal epithelial cells: influence of immunobiotic lactobacilli.2017
Author(s)
Albarracin, L., H. Kobayashi, H. Iida, N. Sato, T. Nochi, H. Aso, S. Salva, S. Alvarez, H. Kitazawa, J. Villena
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Journal Title
Front. Immunol.
Volume: 8(57)
Pages: 1-15
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Draft genome sequence of the immunobiotic strain Lactobacillus jensenii TL2937.2017
Author(s)
Villena, J.,Y. Masumizu, H. Ida, W. Ikeda-Ohtsubo, L. Albarracin, S. Makino, S. Ohkawara, K.i Kimura, L. Saavedra, E. Hebert, H. Kitazawa
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Journal Title
Genome Announc.
Volume: 5(9): e00005-17
Issue: 9
Pages: 1-2
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937: finding new biomarkers for the selection of antiinflammatory immunobiotics.2016
Author(s)
Kobayashi, H., L. Albarracin, N. Sato, P. Kanmani, AKM H. Kober, W. Ikeda-Ohtsubo, Y. Suda, T. Nochi, H. Aso, S. Makino, H. Kano, S. Ohkawara, T. Saito, J. Villena, H. Kitazawa
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Journal Title
Beneficial Microbes, Benef Microbes
Volume: 7
Issue: 5
Pages: 769-782
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Draft Genome Sequence of Lactobacillus plantarum TL2766, a strain with the ability to ferment wakame.2016
Author(s)
Villena, J., L. Saavedra, E. Hebert, Y. Masumizu, N. Sato, AKM H. Kober, L. Albarracin, W. Ikeda-Ohtsubo, S. Makino, K. Kimura, S. Ohkawara, H. Kitazawa
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Journal Title
Genome Announc.
Volume: 4(6): e01328-16
Issue: 6
Pages: 1-2
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Exopolysaacharides of PS in Streptococcus. thermophilus has potentials to against virus infection2019
Author(s)
Hiroya Mizuno, Yasuko Sasaki, Kae Tomotsune, Yuhki Indo, Julio Villena, Leonardo Albarracin, Md Aminul Islam, Wakako Ikeda-Ohtsubo, Katsunori Kimura , Hisashi Aso, Julio Villena, Haruki Kitazawa
Organizer
日本畜産学会第125回大会
Related Report
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[Presentation] Selection of wakame utilizing lactobacilli adhering to porcine intestinal mucin and epitheliocytes2019
Author(s)
Binghui Zhou, Yuki Masumizu, Yuhka Nakano, Yuhki Indo, Ryoya Komatsu, Md Aminul Islam, Wakako Ohtsubo, Yoshihito Suda, Keiichi Suzuki, Hisashi Aso, Haruki Kitazawa
Organizer
日本畜産学会第125回大会
Related Report
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[Presentation] Advanced in vitro evaluation system for selecting anti-diarrheal immunobiotics2019
Author(s)
Ryoya Komatsu, Hikaru Iida, Leonardo Albarracin, Md Aminul Islam, Wakako Ikeda-Ohtsubo, Hisashi Aso, Noriyuki Iwabuchi, Jin-zhong Xiao, Tadao Saito, Julio Villena, Haruki Kitazawa
Organizer
日本畜産学会第125回大会
Related Report
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[Presentation] Immunobiotics research in Japan2019
Author(s)
Haruki Kitazawa
Organizer
SATELLITE SYMPOSIUM "Probiotics: From the laboratory to the market, a national and international vision", University of Concepcion, Chili
Related Report
Int'l Joint Research / Invited
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[Presentation] Comparative study of the immunomodulatory activities of lactobacilli strains in porcine intestinal epithelial cells: effect on the innate antiviral immune response2018
Author(s)
Albarracin, L., V. Garcia-Castillo, H. Iida, N. Sato, P. Kanmani, A. Ilabaca, H. Aso, A. Garcia-Cancino, H. Kitazawa, J. Villena
Organizer
XII Congress of the Latin American Association of Immunology (ALAI) and XXIII Congress of the Mexican Society of Immunology (SMI)
Related Report
Int'l Joint Research
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[Presentation] Development of advanced evaluation system of anti-disease immunobiotics2017
Author(s)
Komatsu, R., H. Iida, AKM. H. Kober, L. Albarracin, Y. Suda, W. Ikeda-Ohtsubo, H. Aso, N. Iwabuchi, J.-z. Xiao, T. Saito, J. Villena, H. Kitazawa
Organizer
日本畜産学会第123回大会 (英語口頭発表)
Related Report
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[Presentation] Effect of immunobiotic Bifidobacteria on the innate antiviral immune response in bovine intestinal epithelial cells: immunotranscriptomic analysis2017
Author(s)
Kober, AKM H., P. Clua, A. Tada, G. Vizoso-Pinto, P. Kanmani, C. Rodriguez, H. Iida, F. Raya-Tonetti, S. Salva, S. Alvarez, V. Melnikov, H. Kitazawa, J. Villena
Organizer
FEMS2017
Related Report
Int'l Joint Research
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[Presentation] Development of immunobiotic evaluation system on porcine intestinal epitheliocytes stimulated by dextran sodium sulfate.2016
Author(s)
Sato, N., AKM H. Kober, H. Kobayashi, A. Tada, H. Iida, Y. Masumizu, W. Ikeda-Ohtsubo, T. Nochi, H. Aso, T. Saito, J. Villena, H. Kitazawa
Organizer
V International Symposium on Lactic Acid Bacteria - Benefitting from lactic acid bacteria, Progress in health and food
Place of Presentation
Tucuman, Argentina
Year and Date
2016-10-19
Related Report
Int'l Joint Research
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[Presentation] Comparative Immunotranscriptomic analysis of two immunobiotic strains with antiviral capabilities in porcine intestinal epithelial cells.2016
Author(s)
Albarracin, L., H. Kobayashi, N. Sato, Y. Masumizu, T. Nochi, H. Aso, S. Salva, S. Alvarez, H. Kitazawa, J. Villena
Organizer
V International Symposium on Lactic Acid Bacteria - Benefitting from lactic acid bacteria, Progress in health and food
Place of Presentation
Tucuman, Argentina
Year and Date
2016-10-19
Related Report
Int'l Joint Research
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[Book] 食と微生物の事典2017
Author(s)
北澤春樹(北本勝ひこ 他編)
Total Pages
512
Publisher
朝倉書店
ISBN
9784254431216
Related Report
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