Investigation of the mechanism of equine herpesvirus-1 latency using mice expressing a viral receptor
| Project/Area Number |
16H05022
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | Hokkaido University |
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Principal Investigator |
Kimura Takashi 北海道大学, 獣医学研究院, 教授 (90261338)
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| Co-Investigator(Kenkyū-buntansha) |
青島 圭佑 北海道大学, 獣医学研究院, 助教 (90745069)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2019: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
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| Keywords | ヘルペスウイルス / ウマ / レセプター / 潜伏感染 |
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| Outline of Final Research Achievements |
Equine herpesvirus-1 (EHV-1) causes respiratory diseases, abortion and myeloencephalopathy in horses. Little is known about the mechanisms underlying latent EHV-1 infection, due to the lack of small animal models and in vitro infection models. In this study, we developed EHV-1-susceptible cell culture and murine systems by transduction of gene encoding EHV-1 entry receptor, and investigated their suitability as models of EHV-1 latent infection. A rat neuronal cell model showing differentiation dependent resistance to EHV-1 infection may be useful to unveiling the molecular mechanisms of EHV-1 neuropathogenicity and latency.
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| Academic Significance and Societal Importance of the Research Achievements |
EHV-1エントリーレセプターであるウマMHCクラスⅠの遺伝子発現により、in vivoにおいてマウスのEHV-1感受性を増強できることが示された。また、EHV-1感染神経細胞モデルを作製したことにより、これまで不明であったEHV-1潜伏感染細胞におけるウイルス遺伝子ならびに宿主遺伝子の発現動態を解析することが可能になった。本細胞モデルを使用した研究を進めることにより、ウイルス潜伏－再活性化とその抑制に関する分子機構がさらに明らかになると期待される。
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Report
(5 results)
Research Products
(26 results)
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[Journal Article] Detection of novel gammaherpesviruses from fruit bats in Indonesia.2018
Author(s)
Wada Y, Sasaki M, Setiyono A, Handharyani E, Rahmadani I, Taha S, Adiani S, Latief M, Kholilullah ZA, Subangkit M, Kobayashi S, Nakamura I, Kimura T, Orba Y, Sawa H
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Journal Title
Journal of Medical Microbiology
Volume: 67(3)
Issue: 3
Pages: 415-422
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Divergent bufavirus harboured in megabats represents a new lineage of parvoviruses2016
Author(s)
Sasaki M, Gonzalez G, Wada Y, Setiyono A, Handharyani E, Rahmadani I, Taha S, Adiani S, Latief M, Kholilullah ZA, Subangkit M, Kobayashi S, Nakamura I, Kimura T, Orba Y, Ito K, Sawa H
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Journal Title
Sci Rep
Volume: 6
Issue: 1
Pages: 24257-24257
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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