• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of genes responsible for mutants caused by chromosome aberration

Research Project

Project/Area Number 16H05050
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Insect science
Research InstitutionIwate University

Principal Investigator

Sahara Ken  岩手大学, 農学部, 教授 (30241368)

Co-Investigator(Kenkyū-buntansha) 安河内 祐二  国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 上級研究員 (50355723)
伊藤 克彦  東京農工大学, (連合)農学研究科(研究院), 准教授 (80725812)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2018: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
KeywordsFISH / カイコ / 染色体変異 / BAC / 工業暗化 / 性フェロモン / 染色体 / 逆位 / 相互転座 / 突然変異 / 突然変異体
Outline of Final Research Achievements

By means of gene/sequence visualizing FISH technique, we addressed to identify the genes/sequences that is responsible for mutant phenotypes and/or aberrant chromosomes in the silkworm. The black moth mutant (strain u41) has been considered to corresponding to the phenotype of industrial melanism (carbonaria) in peppered moth. We found the corresponding chromosome carries inversion and mapped the region into bridged BAC clones. A homeotic mutant silkworm, r52, has reciprocal translocation. We detected 214 bp deletion and other inversions and duplication in the target chromosome sites. Two races, Z and E, has been known in European corn borer which are attracted to different sex pheromones. Linkage analyses have strongly suggested suppression of crossing over within the sex chromosome. Our FISH results no large inversion occurred in the respective region of the Z chromosome.

Academic Significance and Societal Importance of the Research Achievements

カイコをはじめとするチョウ目昆虫の染色体は、切断が起きても分裂サイクルから排除されないため、我々ヒトに比べて放射線に対して格段に強い。その要因の詳細を知ることには重要性がある。ところが、チョウ目昆虫の染色体に起こった変異について分子レベルでの研究は乏しい。そこで我々は、見た目と染色体に突然変異を起こしたカイコ系統の染色体部位特定を行い、変異部分の配列特定を試みた。その結果、工業暗化の原因と目される遺伝子をカイコで研究する方向性を得るとともに2つの染色体が相互に入れ替わった部位に関する知見が得られた。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (37 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (13 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 13 results,  Open Access: 4 results) Presentation (22 results) (of which Int'l Joint Research: 9 results,  Invited: 1 results) Book (1 results)

  • [Int'l Joint Research] Institute of Entomology/Biology Centre CAS(チェコ)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Construction of a BAC library of Endoclita excrescens as a tool for comparative gene mapping in Lepidoptera.2019

    • Author(s)
      Okumura A, Kobayashi M, Kitajima H, Yasukochi Y, Suzuki G, Sahara K
    • Journal Title

      Entomological Science

      Volume: 22 Issue: 2 Pages: 167-172

    • DOI

      10.1111/ens.12350

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Construction of a BAC library and selection of BACs containing orthologs of <i>Bombyx mori</i> genes in <i>Stenopsyche marmorata</i> (Trichoptera: Stenopsychidae)2018

    • Author(s)
      Fujimoto T, Okumura A, Yoshido A, Yasukochi Y, Suzuki G, Sahara K
    • Journal Title

      Journal of Insect Biotechnology and Sericology

      Volume: 87 Issue: 2 Pages: 2_061-2_069

    • DOI

      10.11416/jibs.87.2_061

    • NAID

      130007431343

    • ISSN
      1346-8073, 1884-7978
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Decrease in the expression level of the gene encoding the putative Bombyx mori bidensovirus receptor during virus infection2018

    • Author(s)
      Ito K, Fujii T, Yokoyama T, Kadono-Okuda K.
    • Journal Title

      Arch. Virol.

      Volume: 166 Issue: 12 Pages: 3327-3338

    • DOI

      10.1007/s00705-018-4017-1

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A single amino acid substitution in the Bombyx-specific mucin-like membrane protein causes resistance to Bombyx mori densovirus2018

    • Author(s)
      Ito K, Kidokoro K, Katsuma S, Sezutu H, Uchino K, Kobayashi I, Tamura T, Yamamoto K, Mita K, Shimada T, Kadono-Okuda K
    • Journal Title

      Sci. Rep.

      Volume: 8 Issue: 1 Pages: 7430-7430

    • DOI

      10.1038/s41598-018-25388-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Conservation and lineage-specific rearrangements in the GOBP/PBP gene complex of distantly related ditrysian Lepidoptera2018

    • Author(s)
      Yasukochi Yuji、Yang Bin、Fujimoto Toshiaki、Sahara Ken、Matsuo Takashi、Ishikawa Yukio
    • Journal Title

      PLoSONE

      Volume: 13 Issue: 2 Pages: e0192762-e0192762

    • DOI

      10.1371/journal.pone.0192762

    • NAID

      120006628886

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Linkage analysis and mapping of a gene responsible for the lethal 19 mutation in the silkworm, Bombyx mori.2018

    • Author(s)
      Ito K, Fujii T, Murakami M, Yokoyama T
    • Journal Title

      Journal of Insect Biotechnology and Sericology

      Volume: 87 Pages: 9-16

    • NAID

      130006685530

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Relative humidity and maintenance of p50 silkworms reared on artificial diet.2017

    • Author(s)
      Yuri Kodai, Sahara Ken
    • Journal Title

      Journal of Insect Biotechnology and Sericology

      Volume: 86 Pages: 43-47

    • NAID

      130005865228

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inheritance of cocoon color mutation and heterosis in Antheraea yamamai.2017

    • Author(s)
      Yamada Yasuhiro, Asano Sin-ichiro, Bando Hisanori, Sahara Ken
    • Journal Title

      Journal of Wild Silkworm & Silk

      Volume: 20 Pages: 57-61

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Feminizing Wolbachia endosymbiont disrupts maternal sex chromosome inheritance in a butterfly species2017

    • Author(s)
      Kageyama D, Ohno M, Sasaki T, Yoshido A, Konagaya T, Jouraku A, Kuwazaki S, Kanamori H, Katayose Y, Narita S, Miyata M, Riegler M, Sahara K
    • Journal Title

      Evolution Letters

      Volume: 1 Issue: 5 Pages: 232-244

    • DOI

      10.1002/evl3.28

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Melanin pigmentation gives rise to black spots on the wings of the silkworm Bombyx mori.2016

    • Author(s)
      Ito K, Yoshikawa M, Fujii T, Tabunoki H, Yokoyama T.
    • Journal Title

      Journal of Insect Physiology

      Volume: 91-92 Pages: 100-106

    • DOI

      10.1016/j.jinsphys.2016.07.004

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Variation in nesting behavior of eight species of spider mites, Stigmaeopsis having sociality.2016

    • Author(s)
      Saito Y, Zhang YX, Mori K, Ito K, Sato, Chittenden AR, Lin JZ, Chae Y, Sakagami T, Sahara K
    • Journal Title

      The Science of Nature

      Volume: 103 Issue: 9-10 Pages: 87-87

    • DOI

      10.1007/s00114-016-1408-6

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A histroy of chromosome identification in Bombyx mori2016

    • Author(s)
      Sahara K, Yoshido A, Yasukochi Y
    • Journal Title

      Chromosome Science

      Volume: 19 Pages: 3-9

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The fate of W chromosomes in hybrids between wild silkmoths, Samia cynthia ssp.: no role in sex determination and reproduction.2016

    • Author(s)
      Yoshido A, Marec F, Sahara K
    • Journal Title

      Heredity

      Volume: 116 Issue: 5 Pages: 424-433

    • DOI

      10.1038/hdy.2015.110

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 死蟻蚕の原因遺伝子の単離2019

    • Author(s)
      伊藤 克彦・横山 岳
    • Organizer
      日本蚕糸学会第89回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] カイコ19致死のポジショナルクローニング.2019

    • Author(s)
      松本 祐希名・横山 岳・伊藤 克彦
    • Organizer
      日本蚕糸学会第89回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] カイコの成虫寿命を決定する遺伝子sliの機能解析2019

    • Author(s)
      津田 萌・横山 岳・伊藤 克彦
    • Organizer
      日本蚕糸学会第89回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] カイコ薄紙繭突然変異系統における繭形質の調査と原因遺伝子の探索2019

    • Author(s)
      高橋 優作・横山 岳・伊藤 克彦
    • Organizer
      日本蚕糸学会第89回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Decrease in the expression level of nsd-2 gene encoding the putative Bombyx mori bidensovirus receptor by virus infection.2019

    • Author(s)
      Ito K, Fujii T, Yokoyama T, and Kadono-Okuda K
    • Organizer
      The 6th Asia-Pacific Congress of Sericulture and Insect Biotechnology (APSERI-2019)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] RNA-seqデータからカイコ単一遺伝子オルソログを検出するツールの開発2019

    • Author(s)
      1大野瑞紀・川本宗孝・安河内祐二・佐原健
    • Organizer
      日本蚕糸学会第89回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 毛翅目昆虫ホタルトビケラのBACライブラリー構築2019

    • Author(s)
      藤本章晃・大野瑞紀・倉西良一・安河内祐二・佐原 健
    • Organizer
      日本蚕糸学会第89回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] モノソミーカイコの発見2018

    • Author(s)
      佐原健、松本祐希名、吉戸敦生、川本宗孝、安河内祐二、金児雄
    • Organizer
      東北昆虫学会第1回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] The Z chromosome comparison between Z- and E-races of Ostrinia nubilalis2018

    • Author(s)
      Ohno M, Yamauchi Y, Yasukouchi Y, Yoshido A, Sahara K
    • Organizer
      22nd International chromosome conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] RNA-seq analysis facilitates BAC-FISH mapping2018

    • Author(s)
      Ohno M, Yasukochi Y, Suzuki G, Yoshido A, Sahara K
    • Organizer
      The 10th International Workshop on MOLECULAR BIOLOGY AND GENETICS OF THE LEPIDOPTERA
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A fate of sex chromosomes and its partners in sex-limited yellow cocoon (Sy) strain of Bombyx mori2018

    • Author(s)
      Sahara K, Matsumoto Y, Yoshido A, Kawamoto M, Yasukochi Y, Kaneko Y
    • Organizer
      6th Asia-Pacific Chromosome Colloquium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] BAC-FISH identifies the caddisfly chromosomes2018

    • Author(s)
      Fujimoto T, Kuranishi RB, Yasukochi Y, Suzuki G, Sahara K
    • Organizer
      16th International Symposium on Trichoptera
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Construction of a BAC library and selection of single genes in Colias erate2018

    • Author(s)
      Ohno M, Yasukochi Y, Suzuki G, Sahara K
    • Organizer
      International Symposium on Innovative Agriculture and Fishery (ISIAF2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] First BAC-FISH mapping of the caddisfly (Stenopsyche marmorata)2018

    • Author(s)
      Fujimoto T, Yasukochi Y, Suzuki G, Sahara K
    • Organizer
      nternational Symposium on Innovative Agriculture and Fishery (ISIAF2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] カイコBm系統の原因遺伝子の探索2018

    • Author(s)
      嵯峨新樹・藤本章晃(岩大農)・伊藤克彦・伴野豊・安河内祐二・安藤俊哉・伊藤克彦・佐原健
    • Organizer
      日本蚕糸学会第88回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] カイコ薄紙繭突然変異の繭形質の性状解析とポジショナルクローニング2018

    • Author(s)
      高橋 優作・藤井 毅・横山 岳・伊藤 克彦
    • Organizer
      日本蚕糸学会第88回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] カイコの成虫寿命を短くする遺伝子sliの解析2018

    • Author(s)
      津田 萌・藤井 毅・横山 岳・伊藤 克彦
    • Organizer
      日本蚕糸学会第88回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Genome sequence, characterization and quantification of a novel iflavirus infecting tropical tasar silkworm, Antheraea mylitta2018

    • Author(s)
      7.Kangayam M. Ponnuvel, Katsuhiko Ito, Olle Terenius, Joachim de Miranda, P. Jayaprakash, Kalidas Mandal
    • Organizer
      The 4th Wild silkmoth Conference
    • Related Report
      2017 Annual Research Report
  • [Presentation] コナガのBAC-FISHマッピングとddRAD-seq法による連鎖地図との統合2017

    • Author(s)
      安河内祐二・上樂明也・桑崎誠剛・宮本和久・山本公子・加藤孟宏・佐原健
    • Organizer
      日本蚕糸学会第87回大会
    • Place of Presentation
      筑波産学連携支援センター(茨城県つくば市)
    • Year and Date
      2017-03-21
    • Related Report
      2016 Annual Research Report
  • [Presentation] Expression profile of nsd-2 gene encoding the putative Bombyx mori bidensovirus receptor after virus infection.2017

    • Author(s)
      Katsuhiko Ito, Takeshi Fujii, Takeshi Yokoyama,Keiko Kadono-Okuda
    • Organizer
      Society for Invertebrate Pathology Annual Meeting
    • Related Report
      2017 Annual Research Report
  • [Presentation] Characterization of the moth color mutation, Wild wing spot, in the silkworm Bombyx mori.2016

    • Author(s)
      Ito K, Yoshikawa M, Fujii T, Tabunoki H, Yokoyama T
    • Organizer
      XXV International Congress of Entomology.
    • Place of Presentation
      Orlando, USA
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Sex chromosome evolution in Lepidoptera.2016

    • Author(s)
      Sahara K.
    • Organizer
      Satellite symposium in XXV International Congress of Entomology. Rosen Plaza Hotel, Orlando, USA. Recent Advances in Entomology in Japan--Ecology and Reproductive Biology
    • Place of Presentation
      Orlando. USA
    • Year and Date
      2016-09-25
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] カイコの精巣を用いた染色体と細胞分裂の観察(INカイコの実験単 監修:日本蚕糸学会)2019

    • Author(s)
      吉戸敦生、佐原健
    • Total Pages
      303
    • Publisher
      NTS
    • ISBN
      9784860435981
    • Related Report
      2018 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2022-02-01  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi