• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of integration mechanisms of sex pheromone information in the higher center of insect brains

Research Project

Project/Area Number 16H05053
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Insect science
Research InstitutionKanazawa University

Principal Investigator

Kiya Taketoshi  金沢大学, 生命理工学系, 准教授 (90532309)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2018: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywordsショウジョウバエ / カイコ / Hr38 / 神経活動 / 交尾行動 / fruitless / doublesex / 初期応答遺伝子 / カイコガ / dsx / 性行動 / 性フェロモン / 光遺伝学 / CRISPR / フェロモン
Outline of Final Research Achievements

Responding appropriately to environmental information is essential for the survival and prosperity of animals. Sex pheromone-processing neural pathways of insects is a great model to investigate the relationship between input (sensory information) and corresponding output (sexual behavior). In the present study, I aimed to establish methods to manipulate the activity of neural circuits in an activity-dependent manner using vinegar flies and silkmoths, to elucidate neural pathways of sex pheromone information. I succeeded in labeling and re-activating neural circuits that had been activated by sexual behavior in vinegar flies. In silkmoths, I established strains that can express highly sensitive channel rhodopsin, enabling manipulation of moth behavior by optogenetics.

Academic Significance and Societal Importance of the Research Achievements

昆虫は多様な生得的行動(本能行動)を示しますが、これらの行動がどのような神経回路の働きによって生み出されているのか不明な点が多く残されています。本研究では、モデル昆虫であるショウジョウバエやカイコガを用い、神経活動依存的に発現する遺伝子を利用し、神経回路を可視化・操作する技術を確立しました。本技術を用いて、昆虫の交尾行動時に活動が起きた神経細胞を可視化したり、光照射によって行動を制御することに成功しました。これらの知見は将来、さまざまな昆虫の生得的行動の神経基盤の解明および行動の制御に活用されることが期待されます。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (34 results)

All 2019 2018 2017 2016 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (23 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results) Remarks (5 results) Funded Workshop (1 results)

  • [Journal Article] Activity-dependent visualization and control of neural circuits for courtship behavior in the fly Drosophila melanogaster.2019

    • Author(s)
      Seika Takayanagi-Kiya and Taketoshi Kiya
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 116 Issue: 12 Pages: 5715-5720

    • DOI

      10.1073/pnas.1814628116

    • NAID

      120006605143

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mapping of courtship behavior-induced neural activity in the thoracic ganglia of silkmoth Bombyx mori by an immediate early gene, Hr382018

    • Author(s)
      Koudai Morishita, Masafumi Iwami, Taketoshi Kiya
    • Journal Title

      Zoolog Sci.

      Volume: 印刷中

    • NAID

      120006605144

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Refinement of ectopic protein expression through the GAL4/UAS system in Bombyx mori: application to behavioral and developmental studies2017

    • Author(s)
      6.Hara C, Morishita K, Takayanagi-Kiya S, Mikami A, Uchino K, Sakurai T, KanzakiR, Sezutsu H, Iwami M, Kiya T
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 11796-11796

    • DOI

      10.1038/s41598-017-12102-2

    • NAID

      120006370943

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification and Characterization of Novel Genes Expressed Preferentially in the Corpora Allata or Corpora Cardiaca During the Juvenile Hormone Synthetic Period in the Silkworm, Bombyx mori.2017

    • Author(s)
      Taguchi S, Iwami M, Kiya T.
    • Journal Title

      Zoolog Sci.

      Volume: 34(5) Issue: 5 Pages: 398-405

    • DOI

      10.2108/zs170069

    • NAID

      120006370942

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mblk-1 Transcription Factor Family: Its Roles in Various Animals and Regulation by NOL4 Splice Variants in Mammals.2017

    • Author(s)
      Takayanagi-Kiya S, Kiya T, Kunieda T, Kubo T.
    • Journal Title

      Int J Mol Sci.

      Volume: 18(2) Issue: 2 Pages: 246-246

    • DOI

      10.3390/ijms18020246

    • NAID

      120006226797

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 昆虫の生得的行動を制御する神経機構2018

    • Author(s)
      木矢 剛智
    • Organizer
      第 11回北陸合同バイオシンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 神経活動依存的な遺伝子発現を簡易に定量化できる手法の構築と新規な因子の網羅的スクリーニング2018

    • Author(s)
      八代 百合子、三木 文佳、佐藤 優希、岩見 雅史、木矢 剛智
    • Organizer
      第41回日本分子生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 神経活動依存的に発現する遺伝子Hr38を用いたショウジョウバエ脳において性行動時に活動した神経回路の可視化と操作2018

    • Author(s)
      木矢 星歌、木矢 剛智
    • Organizer
      第41回日本分子生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 神経活動依存的に発現する遺伝子Hr38はCREBの下流で長期記憶の形成を促進する2018

    • Author(s)
      坂本 沙紀、箕口 昌杜、大村 知世、岩見 雅史、木矢 剛智
    • Organizer
      第41回日本分子生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 昆虫の生得的行動を制御する神経機構2017

    • Author(s)
      木矢 剛智
    • Organizer
      第3回ユニークな少数派実験動物を扱う若手が最先端アプローチを勉強する会~The 3rd Animal Geeks~
    • Related Report
      2017 Annual Research Report
  • [Presentation] 神経活動依存的に発現する遺伝子Hr38を用いたショウジョウバエ脳において性行動時に活動した神経回路の可視化と操作2017

    • Author(s)
      木矢 星歌,坂本 沙紀,岩見 雅史,木矢 剛智
    • Organizer
      日本動物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 神経活動依存的に発現する遺伝子Hr38 はCREB の下流で長期記憶の形成を促進する2017

    • Author(s)
      坂本 沙紀,箕口 昌杜,大村 知世,岩見 雅史,木矢 剛智
    • Organizer
      日本動物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 神経活動依存的な遺伝子発現を簡易に定量化できる手法の構築と新規な因子の網羅的スクリーニング2017

    • Author(s)
      八代 百合子,三木 文佳,佐藤 優希,岩見 雅史,木矢 剛智
    • Organizer
      日本動物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] カイコガの脳高次領域においてdoublesex は性特異的に発現する2017

    • Author(s)
      中田 匡美,岩見 雅史,木矢 剛智
    • Organizer
      日本動物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] PTTH 細胞の神経活動がカイコガ幼虫の脱皮タイミングを制御する機構の解析2017

    • Author(s)
      伊藤 瑶子,岩見 雅史,木矢 剛智
    • Organizer
      日本動物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 神経活動依存的な遺伝子発現を簡易に定量化できる手法の構築と新規な因子の網羅的スクリーニング2017

    • Author(s)
      八代 百合子,三木 文佳,佐藤 優希,岩見 雅史,木矢 剛智
    • Organizer
      北陸エピジェネティクス研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 新規な神経活動マーカー遺伝子stripeを利用したショウジョウバエの神経回路の可視化と操作による社会的文脈依存的行動の神経基盤の解析2017

    • Author(s)
      木矢 星歌、塩谷 捺美、西内 巧、岩見 雅史、木矢 剛智
    • Organizer
      日本分子生物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 神経活動依存的な遺伝子発現を簡易に定量化できる手法の構築と新規な因子の網羅的スクリーニング2017

    • Author(s)
      八代 百合子,三木 文佳,佐藤 優希,岩見 雅史,木矢 剛智
    • Organizer
      日本分子生物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 神経活動依存的に発現する遺伝子Hr38 はCREB の下流で長期記憶の形成を促進する2017

    • Author(s)
      坂本 沙紀,箕口 昌杜,大村 知世,岩見 雅史,木矢 剛智
    • Organizer
      日本分子生物学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Visualization and manipulation of neural circuit activated by courtship behavior in the brain of Drosophila melanogaster, using a neural activity marker gene, Hr382016

    • Author(s)
      Seika Takayanagi-Kiya, Masafumi Iwami, Taketoshi Kiya
    • Organizer
      The Joint meeting of the 22nd International Congress of Zoology (ICZ) & the 87th meeting of the ZSJ
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      2016-11-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification of stripe, the insect homolog of egr-1, as a novel immediate early gene in the brain of vinegar fly, Drosophila melanogaster2016

    • Author(s)
      Natsumi Shioya, Seika Takayanagi-Kiya, Takumi Nishiuchi, Masafumi Iwami, Taketoshi Kiya
    • Organizer
      The Joint meeting of the 22nd International Congress of Zoology (ICZ) & the 87th meeting of the ZSJ
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      2016-11-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Characterization of the sexually dimorphic expression of doublesex2016

    • Author(s)
      Masami Nakata, Masafumi Iwami, Taketoshi Kiya
    • Organizer
      The Joint meeting of the 22nd International Congress of Zoology (ICZ) & the 87th meeting of the ZSJ
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      2016-11-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Neural activity-dependent regulation of insect development by PTTH neurons2016

    • Author(s)
      Youko Ito, Masafumi Iwami, Taketoshi Kiya
    • Organizer
      The Joint meeting of the 22nd International Congress of Zoology (ICZ) & the 87th meeting of the ZSJ
    • Place of Presentation
      沖縄コンベンションセンター
    • Year and Date
      2016-11-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Visualization and manipulation of neural circuit activated by courtship behavior in the brain of Drosophila melanogaster, using a neural activity marker gene, Hr382016

    • Author(s)
      木矢剛智, 岩見雅史
    • Organizer
      The 12th Japanese Drosophila Research Conference (JDRC12)
    • Place of Presentation
      立教大学
    • Related Report
      2016 Annual Research Report
  • [Presentation] 活動依存的な神経回路の可視化と操作2016

    • Author(s)
      木矢剛智
    • Organizer
      昆虫ワークショップ2016 (第10回)
    • Place of Presentation
      富士箱根ランド
    • Related Report
      2016 Annual Research Report
  • [Presentation] 神経活動依存的に発現する遺伝子Hr38を用いたショウジョウバエ脳において性行動時に活動した神経回路の可視化と操作2016

    • Author(s)
      木矢剛智, 岩見雅史
    • Organizer
      第39回神経科学大会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2016 Annual Research Report
  • [Presentation] Visualization and manipulation of neural circuit of insect brain using conserved immediate early genes.2016

    • Author(s)
      Taketoshi Kiya
    • Organizer
      The Joint meeting of the 22nd International Congress of Zoology (ICZ) & the 87th meeting of the ZSJ
    • Place of Presentation
      沖縄科学技術大学院大学 OIST
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 神経活動依存的に発現する遺伝子Hr38を用いたショウジョウバエ脳において性行動時に活動した神経回路の可視化と操作2016

    • Author(s)
      木矢剛智, 岩見雅史
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2016 Annual Research Report
  • [Remarks] 昆虫の生得的行動を生み出す神経回路の新しい解析技術を確立!

    • URL

      https://www.kanazawa-u.ac.jp/rd/65392

    • Related Report
      2018 Annual Research Report
  • [Remarks] eurekalert

    • URL

      https://www.eurekalert.org/pub_releases/2019-03/ku-nmf031319.php

    • Related Report
      2018 Annual Research Report
  • [Remarks] 研究室ホームページ

    • URL

      http://kiya.w3.kanazawa-u.ac.jp/

    • Related Report
      2017 Annual Research Report
  • [Remarks]

    • URL

      http://kiya.w3.kanazawa-u.ac.jp/

    • Related Report
      2016 Annual Research Report
  • [Remarks]

    • URL

      http://ridb.kanazawa-u.ac.jp/public/detail.php?id=2397

    • Related Report
      2016 Annual Research Report
  • [Funded Workshop] 第22回国際動物学会シンポジウムOf flies and bees: comparative neuroethology from sensory perception to social behavior in two model specie2016

    • Place of Presentation
      沖縄科学技術大学院大学 OIST
    • Related Report
      2016 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi