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Effects of fluctuation of redox potentials on microbial dynamics and gas metabolisms of wetland soils

Research Project

Project/Area Number 16H05056
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental agriculture(including landscape science)
Research InstitutionNagoya University

Principal Investigator

Murase Jun  名古屋大学, 生命農学研究科, 教授 (30285241)

Co-Investigator(Kenkyū-buntansha) 浅川 晋  名古屋大学, 生命農学研究科, 教授 (50335014)
渡邉 健史  名古屋大学, 生命農学研究科, 講師 (60547016)
Research Collaborator HAYASHI Kentaro  
TOKIDA Takeshi  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2016: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
Keywords水田 / 酸化還元境界層 / 微生物 / メタン / 鉄酸化 / 原生生物 / 微生物群集構造 / 微生物間相互作用 / 酸素 / 微生物相互作用
Outline of Final Research Achievements

Focusing on paddy soil, we analyzed the fluctuation of the redox boundary layer and the response of the microbial community. In the paddy soil surface layer, the dissolved oxygen concentration showed extremely large fluctuation from supersaturation to complete anoxia. The oxidation-reduction potential also changed dramatically in the paddy rice rhizosphere according to the growth of rice. Protozoa living in paddy soils are considered to play an important role as a predator in the redox boundary layer, as there are groups with suitable oxygen concentration or redox potential and a group adaptable to a wide range of oxygen concentrations. Although methane oxidation bacteria require oxygen for methane oxidation, they were able to maintain their activity for a long time even under anaerobic conditions. Microaerobic iron-oxidizing bacteria act in the surface.

Academic Significance and Societal Importance of the Research Achievements

酸化還元境界層は、多くの重要な微生物代謝が進行するホットスポットであるが、厚さ数ミリの酸化還元境界層の中での劇的な化学的濃度勾配に応じた微生物の群集や活性の空間分布の詳細はほとんど分かっていなかった。本研究では、酸化還元境界層が大きなゆらぎを有していることを実証するとともに、境界層で微生物がサブミリスケールの住み分けを行なっている実態を明らかにした。また物質循環の鍵を担う微生物群集の境界層のゆらぎに対する応答を検証した。本研究の成果から、酸化還元境界層における環境変動が微生物の生態や温室効果ガスであるメタンの動態に及ぼす影響を微生物サイズの視点に立ったスケールで解析することの重要性が示された。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (17 results)

All 2019 2018 2017 2016

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (12 results) (of which Int'l Joint Research: 5 results,  Invited: 3 results) Book (1 results)

  • [Journal Article] Ferrigenium kumadai gen. nov., sp. nov., a microaerophilic iron-oxidizing bacterium isolated from a paddy field soi2018

    • Author(s)
      Khalifa, A., Nakasuji, Y., Saka, N., Honjo, H., Asakawa, S., and Watanabe, T.
    • Journal Title

      Int. J. Syst. Evol. Microbiol.

      Volume: 68 Issue: 8 Pages: 2587-2592

    • DOI

      10.1099/ijsem.0.002882

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Quest of Soil Protists in a New Era2017

    • Author(s)
      Murase, Jun
    • Journal Title

      Microbes and Environments

      Volume: 32 Issue: 2 Pages: 99-102

    • DOI

      10.1264/jsme2.ME3202rh

    • NAID

      130006789904

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Microhabitat segregation of heterotrophic protists in the rice (Oryza sativa L.) rhizosphere2017

    • Author(s)
      Asiloglu, Rasit Murase, Jun
    • Journal Title

      Rhizosphere

      Volume: 4 Pages: 82-88

    • DOI

      10.1016/j.rhisph.2017.08.001

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Response of the protistan community of a rice field soil to different oxygen tensions2016

    • Author(s)
      Takenouchi, Y. Iwasaki, K. Murase, J.
    • Journal Title

      FEMS Microbiology Ecology

      Volume: 92 Issue: 7 Pages: fiw104-fiw104

    • DOI

      10.1093/femsec/fiw104

    • NAID

      120005895264

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] 水田生態系から分離されたメタン酸化細菌の亜酸化窒素生成能2019

    • Author(s)
      Mohammad Saiful Alam、Joseph Benewinde Sawadogo、浅川 晋
    • Organizer
      日本土壌微生物学会2019年度札幌大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 湛水水田土壌表層における溶存酸素鉛直プロファイルの昼夜変動2018

    • Author(s)
      村瀬 潤、後藤佳介、杉浦大輔
    • Organizer
      日本土壌肥料学会中部支部第97回例会
    • Related Report
      2018 Annual Research Report
  • [Presentation] メタン生成古細菌 Methanosarcina mazei のメタノコンドロイチン分解酵素の解析2018

    • Author(s)
      楠 悠李, 難波竜士, 和田隆太郎, 吉田稜, 江見晃一, 小川 拓哉, 邊見 久, 浅川 晋
    • Organizer
      日本Archaea研究会第31回講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Ecology of protists in a rice rhizosphere2018

    • Author(s)
      Jun Murase
    • Organizer
      International Symposium in Mollisols Ecology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Microbial food chain driven by methane in a rice field soil2018

    • Author(s)
      Jun Murase
    • Organizer
      The 8th East Asian Federation of Ecological Societies
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Methane-driven microbial food web in the rice rhizosphere2017

    • Author(s)
      Yuko Hibino, Takeshi Tokida, Takashi Okubo, Miwa Arai, Kentaro Hayashi, Hidemitsu Sakai, Toshihiro Hasegawa, Jun Murase
    • Organizer
      13th International Conference of East and Southeast Asia Federation of Soil Science Society
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 水田土壌のメタン酸化微生物食物連鎖の構造と機能2017

    • Author(s)
      村瀬潤, 日々野優子, 常田岳志, 大久保卓, 荒井美和, 林健太郎, 酒井英光, 長谷川利拡
    • Organizer
      日本地球惑星科学連合2017年大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 水稲根圏におけるメタン酸化食物連鎖の安定同位体プロービング法による解析2017

    • Author(s)
      日比野優子, 常田岳志, 大久保卓, 荒井美和, 林健太郎, 酒井英光, 長谷川利拡, 村瀬潤
    • Organizer
      日本土壌肥料学会2017年度大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 水稲根圏における原生生物の生態と機能2017

    • Author(s)
      村瀬潤
    • Organizer
      大気-森林-土壌循環ワークショップ
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] メタン酸化が駆動する水田の微生物食物連鎖の構造は根圏と非根圏で 異なる2016

    • Author(s)
      日比野優子, 常田岳志, 大久保卓, 荒井見和, 林健太郎, 酒井英光, 長谷川利拡, 村瀬潤
    • Organizer
      日本微生物生態学会第 31 回横須賀大会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Response of the protistan community of a rice field soil to different oxygen tensions2016

    • Author(s)
      Jun Murase, Kazufumi Iwasaki, Yuriko Takenouchi
    • Organizer
      16th International Symposium on Microbial Ecology (ISME 16)
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Diversity and ecology of microeukaryotes in rice field soil2016

    • Author(s)
      Jun Murase
    • Organizer
      The 8th Asian Symposium on Microbial Ecology
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] 実践土壌学 1 土壌微生物学2018

    • Author(s)
      村瀬 潤(分担執筆)豊田剛己(編)
    • Total Pages
      194
    • Publisher
      朝倉書店
    • ISBN
      9784254435719
    • Related Report
      2018 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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