| Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2018: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2017: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2016: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
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| Outline of Final Research Achievements |
We had found that nitrous oxide is emitted from not only soil but also unharvested crop residues on a cabbage field, and hypothesized that denitrification occurs in the crop residue. As a result, some plant indigenous bacteria were isolated as denitrifiers from the crop residue, and the isolates showed denitrification activity under an aerobic (static) condition. Genomic features were not identical among the isolates: some of the isolates encoded nitrous oxide reductase gene on their genomes while the others didn't. A high amount of nitrate was detected in the residue. Culturing experiments with the residue showed cell growth of the isolate was observed with a reduction of nitrate concomitant with a release of nitrous oxide. Moreover, some sugars and organic acids contained in the residue were shown to be electron donors for the denitrifiers. These results suggest that some plant indigenous bacteria can utilize components of the crop residue to denitrify and produce nitrous oxide.
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