Molecular understanding of self/non-self recognition mechanism on plant reproduction.

• Project/Area Number: 16H05066
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Mie University
• Principal Investigator: Suwabe Keita 三重大学, 生物資源学研究科, 准教授 (50451612)
• Co-Investigator(Kenkyū-buntansha): 加賀谷 安章 三重大学, 地域イノベーション推進機構, 准教授 (20335152)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000); Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: 遺伝学 / 植物生殖 / 花粉認識機構 / 受粉 / シロイヌナズナ / 自家不和合性 / トランスクリプトーム

Outline of Final Research Achievements

How do plant species accomplish a successful reproduction to produce next generations? This is a long-term issue for plant biologists to be solved since T. Knight and C. Darwin found a basis of this attractive plant reproduction system. In this research project, we focus on a molecular mechanism of the self-incompatibility in Arabidopsis thaliana, as a model of self/non-self recognition mechanism on plant reproduction. By a combination of genetic and reverse genetic analyses, we found that a downstream system, after self/non-self pollen recognition, is controlled by multiple and hierarchical mechanisms and pollen rejection is synergistically regulated by pollen acceptance mechanisms, such as water transport and pollen tube germination, on pollination.

Academic Significance and Societal Importance of the Research Achievements

植物が種の存続と繁栄を両立させるために進化の過程で獲得した生殖戦略メカニズムである自家不和合性は、トーマス・ナイトやチャールズ・ダーウィンによる提唱から140年を経た現在においてもその分子メカニズムはほとんど解明されていない。このような歴史的背景に対し、本研究はその本質を司る因子と分子メカニズムの一端を明らかにした。本成果は、受粉人為コントロールや自家不和合性安定化によるアブラナ科野菜の新採種技術確立のための情報基盤として波及できる。

Research Products

• [Int'l Joint Research] Chungnam National University/Sunchon National University(韓国)
• [Int'l Joint Research] Sunchon National University/Chungnum National University(韓国)
• [Int'l Joint Research] Sunchon National University/Chungnum National Uuniversity(韓国)
• [Journal Article] Genetic and tissue-specific RNA-sequencing analysis of self-compatible mutant TSC28 in <i>Brassica rapa</i> L. toward identification of a novel self-incompatibility factor (2019)
  • Author(s): Osaka, M., Nabemoto, M., Maeda, S., Sakazono, S., Masuko-Suzuki, H., Ito, K., Takada, Y., Kobayashi, I., Lim, Y. P., Nakazono, M., Fujii, S., Murase, K., Takayama, S., Suzuki, G., Suwabe, K., and Watanabe, M.
  • Journal Title: Genes & Genetic Systems Volume: 94 Issue: 4 Pages: 167-176
  • DOI: 10.1266/ggs.19-00010
  • NAID: 130007738050
  • ISSN: 1341-7568, 1880-5779
  • Year and Date: 2019-08-01
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Abscisic acid-mediated developmental flexibility of stigmatic papillae in response to ambient humidity in <i>Arabidopsis thaliana</i> (2018)
  • Author(s): Takeda, S., Ochiai, K., Egusa, W., Morimoto, H., Sakazono, S., Osaka, M., Nabemoto, M., Kagaya, Y., Suzuki, G., Watanabe, M., and Suwabe, K.
  • Journal Title: Genes & Genetic Systems Volume: 93 Issue: 5 Pages: 209-220
  • DOI: 10.1266/ggs.18-00025
  • NAID: 130007547247
  • ISSN: 1341-7568, 1880-5779
  • Year and Date: 2018-10-01
  • Peer Reviewed / Open Access
• [Journal Article] Duplicated pollen-pistil recognition loci control intraspecific unilateral incompatibility in Brassica rapa (2017)
  • Author(s): Takada Yoshinobu、Murase Kohji、Shimosato-Asano Hiroko、Sato Takahiro、Nakanishi Honoka、Suwabe Keita、Shimizu Kentaro K.、Lim Yong Pyo、Takayama Seiji、Suzuki Go、Watanabe Masao
  • Journal Title: Nature Plants Volume: 3 Issue: 7 Pages: 17096-17096
  • DOI: 10.1038/nplants.2017.96
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comparative analysis of microRNA profiles of rice anthers between cool-sensitive and cool-tolerant cultivars under cool-temperature stress (2016)
  • Author(s): Shunsuke Maeda, Satomi Sakazono, Hiromi Masuko-Suzuki, Midori Taguchi, Kaori Yamamura, Kuniaki Nagano, Takashi Endo, Kenichi Saeki, Masaaki Osaka, Moe Nabemoto, Kana Ito, Toru Kudo, Masaaki Kobayashi, Mizuho Kawagishi, Kotomi Fujita, Hikaru Nanjo, Tomoe Shindo, Kentaro Yano, Go Suzuki, Keita Suwabe, and Masao Watanabe
  • Journal Title: Genes & Genetic Systems Volume: 91 Issue: 2 Pages: 97-109
  • DOI: 10.1266/ggs.15-00056
  • NAID: 130005421208
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of reference genes for quantitative expression analysis using large-scale RNA-seq data of <i>Arabidopsis thaliana</i> and model crop plants (2016)
  • Author(s): Toru Kudo, Yohei Sasaki, Shin Terashima, Noriko Matsuda-Imai, Tomoyuki Takano, Misa Saito, Maasa Kanno, Soichi Ozaki, Keita Suwabe, Go Suzuki, Masao Watanabe, Makoto Matsuoka, Seiji Takayama, Kentaro Yano
  • Journal Title: Genes & Genetic Systems Volume: 91 Issue: 2 Pages: 111-125
  • DOI: 10.1266/ggs.15-00065
  • NAID: 130005421205
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Genomic structural variation in the self-incompatibility locus causes a transition from outcrossing to selfing in amphidiploid Arabidopsis kamchatica. (2019)
  • Author(s): Ota, T., Kagaya, Y., Suzuki, G., Watanabe, M., and Suwabe, K.
  • Organizer: 4th Edition of Global Conference on Plant Science and Molecular Biology
  • Int'l Joint Research / Invited
• [Presentation] Morphological aspect and molecular mechanism of Pollen hydration on pollination in Arabidopsis thaliana. (2019)
  • Author(s): Takada, M., Ota, T., Mizoguchi, Y., Ando, M., Kagaya, Y., Suzuki, G., Watanabe, M., and Suwabe, K.
  • Organizer: 4th Edition of Global Conference on Plant Science and Molecular Biology
  • Int'l Joint Research / Invited
• [Presentation] Developmental flexibility of stigmatic papilla cell in response to abiotic stress in Arabidopsis thaliana. (2019)
  • Author(s): Egusa, W., Takada, M., Takeda, S., Ochiai, K., Sakazono, S., Osaka, M., Kagaya, Y., Suzuki, G., Watanabe, M. and Suwabe, K.
  • Organizer: Plant and Animal Genome XXVII
  • Int'l Joint Research
• [Presentation] Pollen hydration mechanism on pollination in Arabidopsis thaliana. (2019)
  • Author(s): Ota, T., Mizoguchi, Y., Ando, M., Kagaya, Y., Suzuki, G., Watanabe, M. and Suwabe, K.
  • Organizer: Plant and Animal Genome XXVII
  • Int'l Joint Research
• [Presentation] Morphological aspect and molecular mechanism of pollen hydration on pollination in Arabidopsis thaliana. (2018)
  • Author(s): Ota, T., Mizoguchi, Y., Ando, M., Kagaya, Y., Suzuki, G., Watanabe, M. and Suwabe, K.
  • Organizer: International Plant Molecular Biology 2018
  • Int'l Joint Research
• [Presentation] Novel unilateral incompatibility in Brassica rapa is regulated by duplicated self-incompatibility genes, PUI1 and SUI1. (2018)
  • Author(s): Takada, Y., Murase, K., Shimosato-Adano, H., Sato, T., Nakanishi, H., Suwabe, K., Shimizu, K. K., Lim, Y. P., Takayama, S., Suzuki, G., and Watanabe, M.
  • Organizer: Plant & Animal Genome XXVI
  • Int'l Joint Research / Invited
• [Presentation] ABA-mediated flexibility of female stigmatic papillae development in Arabidopsis thaliana. (2017)
  • Author(s): W. Egusa, S. Takeda, K. Ochiai, H. Morimoto, S. Sakazono, M. Osaka, M. Nabemoto, Y. Kagaya, G. Suzuki, M. Watanabe and K. Suwabe
  • Organizer: International Plant & Animal Genome XXV
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2017-01-14
  • Int'l Joint Research
• [Presentation] Pollen-cysteine rich peptide CR3 regulates pollen germination in Arabidopsis thaliana. (2017)
  • Author(s): H. Morimoto, W. Egusa, C. Arakawa, H. Masuko-Suzuki, G. Suzuki, M. Watanabe and K. Suwabe
  • Organizer: International Plant & Animal Genome XXV
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2017-01-14
  • Int'l Joint Research
• [Presentation] ABA-mediated stress response mechanism modulates stigmatic papillae development in Arabidopsis thaliana. (2017)
  • Author(s): Egusa, W., Takeda, S., Ochiai, K., Morimoto, H., Kagaya, Y., Suzuki, G., Watanabe, M., and Suwabe, K.
  • Organizer: Global Conference on Plant Science and Molecular Biology
  • Int'l Joint Research
• [Presentation] Identification and characterization of the novel pollen-stigma recognition factors for unilateral incompatibility in Brassica rapa. (2017)
  • Author(s): Takada, Y., Murase, K., Shimosato-Adano, H., Sato, T., Nakanishi, H., Suwabe, K., Shimizu, K. K., Lim, Y. P., Takayama, S., Suzuki, G., and Watanabe, M.
  • Organizer: Taiwan-Japan Plant Biology 2017
  • Int'l Joint Research / Invited
• [Presentation] Brassica rapaの種内一側性不和合性を制御するSUI1-PUI1遺伝子 (2017)
  • Author(s): 高田美信, 鈴木剛, 村瀬浩司, 浅野(下里)裕子, 佐藤陽洋, 中西ほのか, 諏訪部圭太, Lim Yong Pyo, 清水健太郎, 高山誠司, 渡辺正夫
  • Organizer: 日本植物細胞分子生物学会
• [Presentation] 自己花粉を認識するリガンド・レセプター遺伝子セットの重複が非自己花粉の拒絶を引き起こす (2017)
  • Author(s): 高田美信, 鈴木剛, 中西ほのか, 村瀬浩司, 浅野(下里)裕子, 佐藤陽洋, 諏訪部圭太, Lim Yong Pyo, 清水健太郎, 高山誠司, 渡辺正夫
  • Organizer: 日本遺伝学会
• [Presentation] Brassica rapaのA04染色体には新規生殖障壁遺伝子が存在する (2017)
  • Author(s): 高田美信, 中西ほのか, 村瀬浩司, 浅野(下里)裕子, 佐藤陽洋, Yong Pyo Lim, 清水健太郎, 高山誠司, 諏訪部圭太, 鈴木剛, 渡辺正夫
  • Organizer: 日本染色体学会
• [Presentation] Brassica rapaの種内一側性不和合性を支配する花粉・柱頭認識因子の決定 (2017)
  • Author(s): 高田美信, 村瀬浩二, 浅野(下里)裕子, 佐藤陽洋, 中西ほのか, 諏訪部圭太, Lim Yong Pyo, 清水健太郎, 高山誠司, 鈴木剛, 渡辺正夫
  • Organizer: 日本育種学会第132回講演会
• [Presentation] レーザーマイクロダイセクション-マイクロアレイ法によるトマト高温肥大不良果発生に関連する遺伝子の網羅的探索 (2017)
  • Author(s): 長菅香織, 諏訪部圭太, 高橋宏和, 矢野加奈子, 上野広樹, 松尾哲, 今西俊介
  • Organizer: 園芸学会平成29年度秋季大会
• [Presentation] アブラナ科植物の穂発芽抑制メカニズムの解明 (2017)
  • Author(s): 山口裕子、笹岡万優子、行本尭史、加賀谷安章
  • Organizer: 第7回地域イノベーション学会大会
• [Presentation] 抽苔特性に影響せず早く発芽する種子の開発 (2017)
  • Author(s): 塩崎智裕、藤田明香里、加賀谷安章
  • Organizer: 第7回地域イノベーション学会大会
• [Presentation] CR3, a pollen cysteine-rich peptide, induces pollen germination. (2016)
  • Author(s): H. Morimoto, W. Egusa, C. Arakawa, H. Masuko-Suzuki, G. Suzuki, M. Watanabe and K. Suwabe
  • Organizer: The 27th International Conference on Arabidopsis Research
  • Place of Presentation: Gyeongju, Korea
  • Year and Date: 2016-06-29
  • Int'l Joint Research