Molecular understanding of self/non-self recognition mechanism on plant reproduction.
Project/Area Number |
16H05066
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied molecular and cellular biology
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Research Institution | Mie University |
Principal Investigator |
Suwabe Keita 三重大学, 生物資源学研究科, 准教授 (50451612)
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Co-Investigator(Kenkyū-buntansha) |
加賀谷 安章 三重大学, 地域イノベーション推進機構, 准教授 (20335152)
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Project Period (FY) |
2016-04-01 – 2020-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
|
Keywords | 遺伝学 / 植物生殖 / 花粉認識機構 / 受粉 / シロイヌナズナ / 自家不和合性 / トランスクリプトーム |
Outline of Final Research Achievements |
How do plant species accomplish a successful reproduction to produce next generations? This is a long-term issue for plant biologists to be solved since T. Knight and C. Darwin found a basis of this attractive plant reproduction system. In this research project, we focus on a molecular mechanism of the self-incompatibility in Arabidopsis thaliana, as a model of self/non-self recognition mechanism on plant reproduction. By a combination of genetic and reverse genetic analyses, we found that a downstream system, after self/non-self pollen recognition, is controlled by multiple and hierarchical mechanisms and pollen rejection is synergistically regulated by pollen acceptance mechanisms, such as water transport and pollen tube germination, on pollination.
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Academic Significance and Societal Importance of the Research Achievements |
植物が種の存続と繁栄を両立させるために進化の過程で獲得した生殖戦略メカニズムである自家不和合性は、トーマス・ナイトやチャールズ・ダーウィンによる提唱から140年を経た現在においてもその分子メカニズムはほとんど解明されていない。このような歴史的背景に対し、本研究はその本質を司る因子と分子メカニズムの一端を明らかにした。本成果は、受粉人為コントロールや自家不和合性安定化によるアブラナ科野菜の新採種技術確立のための情報基盤として波及できる。
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Report
(5 results)
Research Products
(26 results)
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[Journal Article] Genetic and tissue-specific RNA-sequencing analysis of self-compatible mutant TSC28 in <i>Brassica rapa</i> L. toward identification of a novel self-incompatibility factor2019
Author(s)
Osaka, M., Nabemoto, M., Maeda, S., Sakazono, S., Masuko-Suzuki, H., Ito, K., Takada, Y., Kobayashi, I., Lim, Y. P., Nakazono, M., Fujii, S., Murase, K., Takayama, S., Suzuki, G., Suwabe, K., and Watanabe, M.
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Journal Title
Genes & Genetic Systems
Volume: 94
Issue: 4
Pages: 167-176
DOI
NAID
ISSN
1341-7568, 1880-5779
Year and Date
2019-08-01
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Abscisic acid-mediated developmental flexibility of stigmatic papillae in response to ambient humidity in <i>Arabidopsis thaliana</i>2018
Author(s)
Takeda, S., Ochiai, K., Egusa, W., Morimoto, H., Sakazono, S., Osaka, M., Nabemoto, M., Kagaya, Y., Suzuki, G., Watanabe, M., and Suwabe, K.
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Journal Title
Genes & Genetic Systems
Volume: 93
Issue: 5
Pages: 209-220
DOI
NAID
ISSN
1341-7568, 1880-5779
Year and Date
2018-10-01
Related Report
Peer Reviewed / Open Access
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[Journal Article] Comparative analysis of microRNA profiles of rice anthers between cool-sensitive and cool-tolerant cultivars under cool-temperature stress2016
Author(s)
Shunsuke Maeda, Satomi Sakazono, Hiromi Masuko-Suzuki, Midori Taguchi, Kaori Yamamura, Kuniaki Nagano, Takashi Endo, Kenichi Saeki, Masaaki Osaka, Moe Nabemoto, Kana Ito, Toru Kudo, Masaaki Kobayashi, Mizuho Kawagishi, Kotomi Fujita, Hikaru Nanjo, Tomoe Shindo, Kentaro Yano, Go Suzuki, Keita Suwabe, and Masao Watanabe
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Journal Title
Genes & Genetic Systems
Volume: 91
Issue: 2
Pages: 97-109
DOI
NAID
ISSN
1341-7568, 1880-5779
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Identification of reference genes for quantitative expression analysis using large-scale RNA-seq data of <i>Arabidopsis thaliana</i> and model crop plants2016
Author(s)
Toru Kudo, Yohei Sasaki, Shin Terashima, Noriko Matsuda-Imai, Tomoyuki Takano, Misa Saito, Maasa Kanno, Soichi Ozaki, Keita Suwabe, Go Suzuki, Masao Watanabe, Makoto Matsuoka, Seiji Takayama, Kentaro Yano
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Journal Title
Genes & Genetic Systems
Volume: 91
Issue: 2
Pages: 111-125
DOI
NAID
ISSN
1341-7568, 1880-5779
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Morphological aspect and molecular mechanism of Pollen hydration on pollination in Arabidopsis thaliana.2019
Author(s)
Takada, M., Ota, T., Mizoguchi, Y., Ando, M., Kagaya, Y., Suzuki, G., Watanabe, M., and Suwabe, K.
Organizer
4th Edition of Global Conference on Plant Science and Molecular Biology
Related Report
Int'l Joint Research / Invited
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[Presentation] Developmental flexibility of stigmatic papilla cell in response to abiotic stress in Arabidopsis thaliana.2019
Author(s)
Egusa, W., Takada, M., Takeda, S., Ochiai, K., Sakazono, S., Osaka, M., Kagaya, Y., Suzuki, G., Watanabe, M. and Suwabe, K.
Organizer
Plant and Animal Genome XXVII
Related Report
Int'l Joint Research
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[Presentation] Pollen hydration mechanism on pollination in Arabidopsis thaliana.2019
Author(s)
Ota, T., Mizoguchi, Y., Ando, M., Kagaya, Y., Suzuki, G., Watanabe, M. and Suwabe, K.
Organizer
Plant and Animal Genome XXVII
Related Report
Int'l Joint Research
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[Presentation] Morphological aspect and molecular mechanism of pollen hydration on pollination in Arabidopsis thaliana.2018
Author(s)
Ota, T., Mizoguchi, Y., Ando, M., Kagaya, Y., Suzuki, G., Watanabe, M. and Suwabe, K.
Organizer
International Plant Molecular Biology 2018
Related Report
Int'l Joint Research
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[Presentation] Novel unilateral incompatibility in Brassica rapa is regulated by duplicated self-incompatibility genes, PUI1 and SUI1.2018
Author(s)
Takada, Y., Murase, K., Shimosato-Adano, H., Sato, T., Nakanishi, H., Suwabe, K., Shimizu, K. K., Lim, Y. P., Takayama, S., Suzuki, G., and Watanabe, M.
Organizer
Plant & Animal Genome XXVI
Related Report
Int'l Joint Research / Invited
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[Presentation] ABA-mediated flexibility of female stigmatic papillae development in Arabidopsis thaliana.2017
Author(s)
W. Egusa, S. Takeda, K. Ochiai, H. Morimoto, S. Sakazono, M. Osaka, M. Nabemoto, Y. Kagaya, G. Suzuki, M. Watanabe and K. Suwabe
Organizer
International Plant & Animal Genome XXV
Place of Presentation
San Diego, CA, USA
Year and Date
2017-01-14
Related Report
Int'l Joint Research
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[Presentation] ABA-mediated stress response mechanism modulates stigmatic papillae development in Arabidopsis thaliana.2017
Author(s)
Egusa, W., Takeda, S., Ochiai, K., Morimoto, H., Kagaya, Y., Suzuki, G., Watanabe, M., and Suwabe, K.
Organizer
Global Conference on Plant Science and Molecular Biology
Related Report
Int'l Joint Research
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[Presentation] Identification and characterization of the novel pollen-stigma recognition factors for unilateral incompatibility in Brassica rapa.2017
Author(s)
Takada, Y., Murase, K., Shimosato-Adano, H., Sato, T., Nakanishi, H., Suwabe, K., Shimizu, K. K., Lim, Y. P., Takayama, S., Suzuki, G., and Watanabe, M.
Organizer
Taiwan-Japan Plant Biology 2017
Related Report
Int'l Joint Research / Invited
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[Presentation] Brassica rapaの種内一側性不和合性を制御するSUI1-PUI1遺伝子2017
Author(s)
高田美信, 鈴木剛, 村瀬浩司, 浅野(下里)裕子, 佐藤陽洋, 中西ほのか, 諏訪部圭太, Lim Yong Pyo, 清水健太郎, 高山誠司, 渡辺正夫
Organizer
日本植物細胞分子生物学会
Related Report
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[Presentation] 自己花粉を認識するリガンド・レセプター遺伝子セットの重複が非自己花粉の拒絶を引き起こす2017
Author(s)
高田美信, 鈴木剛, 中西ほのか, 村瀬浩司, 浅野(下里)裕子, 佐藤陽洋, 諏訪部圭太, Lim Yong Pyo, 清水健太郎, 高山誠司, 渡辺正夫
Organizer
日本遺伝学会
Related Report
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[Presentation] Brassica rapaのA04染色体には新規生殖障壁遺伝子が存在する2017
Author(s)
高田美信, 中西ほのか, 村瀬浩司, 浅野(下里)裕子, 佐藤陽洋, Yong Pyo Lim, 清水健太郎, 高山誠司, 諏訪部圭太, 鈴木剛, 渡辺正夫
Organizer
日本染色体学会
Related Report
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[Presentation] Brassica rapaの種内一側性不和合性を支配する花粉・柱頭認識因子の決定2017
Author(s)
高田美信, 村瀬浩二, 浅野(下里)裕子, 佐藤陽洋, 中西ほのか, 諏訪部圭太, Lim Yong Pyo, 清水健太郎, 高山誠司, 鈴木剛, 渡辺正夫
Organizer
日本育種学会第132回講演会
Related Report
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