Study on DNA binding PPR protein
| Project/Area Number |
16H05067
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2018: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
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| Keywords | PPR / オルガネラ / 遺伝子発現 / pentatricopeptide repeat / 葉緑体 / ミトコンドリア / ゲノム編集 / pentatricopeptide |
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| Outline of Final Research Achievements |
This study has been conducted to understand the principle of the molecular function of DNA binding PPR protein (dPPRP), in contrast to the major class of PPR protein contains RNA binding capacity (RNA binding PPR protein; rPPRP). Dozens of dPPRP gene were selected in this study. The sub cellular localization was found to be in mitochondria or chloroplast as similar to rPPRP. The dPPRP exhibited sequence specific DNA binding activity as expected. The physiological phenotypes were severe in several dPPRP deficient Arabidopsis. The sequence analysis using public database identified several significant amino acids that cloud involved in the evolution of dPPRP genes and/or functional divergence between dPPRP and rPPRP.
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| Academic Significance and Societal Importance of the Research Achievements |
多くのPPRタンパク質がRNA結合に働くのに対して、一部のPPRタンパク質がDNA結合に働くことを見出しており、本研究は当該知見に立脚して、植物にのみ豊富に存在するPPRタンパク質遺伝子に関する理解を深めるものである。本研究の知見は、PPRタンパク質のDNAまたはRNA結合を支える分子基盤、PPRタンパク質機能多様化の理解、ひいてはオルガネラゲノム構造・発現の新知見を与えるものである。さらに本知見は、ゲノム編集技術開発のための基礎知的基盤としても有用である。
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Report
(4 results)
Research Products
(9 results)
