Study on DNA binding PPR protein

• Project/Area Number: 16H05067
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Kyushu University
• Principal Investigator: Nakamura Takahiro 九州大学, 農学研究院, 准教授 (10464398)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000); Fiscal Year 2018: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2017: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2016: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
• Keywords: PPR / オルガネラ / 遺伝子発現 / pentatricopeptide repeat / 葉緑体 / ミトコンドリア / ゲノム編集 / pentatricopeptide

Outline of Final Research Achievements

This study has been conducted to understand the principle of the molecular function of DNA binding PPR protein (dPPRP), in contrast to the major class of PPR protein contains RNA binding capacity (RNA binding PPR protein; rPPRP). Dozens of dPPRP gene were selected in this study. The sub cellular localization was found to be in mitochondria or chloroplast as similar to rPPRP. The dPPRP exhibited sequence specific DNA binding activity as expected. The physiological phenotypes were severe in several dPPRP deficient Arabidopsis. The sequence analysis using public database identified several significant amino acids that cloud involved in the evolution of dPPRP genes and/or functional divergence between dPPRP and rPPRP.

Academic Significance and Societal Importance of the Research Achievements

多くのPPRタンパク質がRNA結合に働くのに対して、一部のPPRタンパク質がDNA結合に働くことを見出しており、本研究は当該知見に立脚して、植物にのみ豊富に存在するPPRタンパク質遺伝子に関する理解を深めるものである。本研究の知見は、PPRタンパク質のDNAまたはRNA結合を支える分子基盤、PPRタンパク質機能多様化の理解、ひいてはオルガネラゲノム構造・発現の新知見を与えるものである。さらに本知見は、ゲノム編集技術開発のための基礎知的基盤としても有用である。

Research Products

• [Journal Article] Comprehensive prediction of target RNA editing sites for PLS-class PPR proteins in Arabidopsis thaliana. (2019)
  • Author(s): Kobayashi T, Yagi Y, Nakamura T
  • Journal Title: Plant Cell Physiol Volume: in press Issue: 4 Pages: 862-874
  • DOI: 10.1093/pcp/pcy251
  • Peer Reviewed
• [Journal Article] 新規ゲノム編集 (2018)
  • Author(s): 田村泰造、中村崇裕
  • Journal Title: DOJIN BIOSCIENCE SERIES ゲノム編集とその医療応用 Volume: 印刷中
• [Journal Article] PPR技術を利用した新しいDNA/RNA操作ツールの開発 (2016)
  • Author(s): 中村崇裕
  • Journal Title: 実験医学増刊・All aboutゲノム編集 Volume: 34 Pages: 180-184
• [Presentation] PPR蛋白質を利用したDNA/RNA編集技術の開発 (2019)
  • Author(s): 中村崇裕
  • Organizer: 産学官連携秋季シンポジウム
  • Invited
• [Presentation] PPR技術で創るDNA/RNA編集技術 (2019)
  • Author(s): 中村崇裕
  • Organizer: 国際医薬品開発展
  • Invited
• [Presentation] Development of New DNA/RNA manipulation Tools Based on PPR Proteins (2018)
  • Author(s): 中村崇裕
  • Organizer: JAACT2018
  • Int'l Joint Research / Invited
• [Presentation] 国産ゲノム・RNA編集技術の医療での展開 (2018)
  • Author(s): 中村崇裕
  • Organizer: 革新的医薬・核酸医薬の開発
  • Invited
• [Presentation] PPR motif as a New DNA/RNA Binding Module for Genome/transcriptome Editing (2017)
  • Author(s): 中村崇裕
  • Organizer: AFELiSA
  • Int'l Joint Research / Invited
• [Patent(Industrial Property Rights)] PPR蛋白質を利用したDNA結合性タンパク質およびその利用 (2017)
  • Inventor(s): 中村崇裕、八木祐介、山根昌之
  • Industrial Property Rights Holder: 中村崇裕、八木祐介、山根昌之
  • Industrial Property Rights Type: 特許
  • Filing Date: 2017
  • Overseas